Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process

Resting cysts play crucial roles in the ecology of dino?agellates, especially in wintering or surviving unfavorable conditions, seeding harmful algal blooms(HABs), and facilitating the geographic expansion. Encystment of dino?agellates is tightly coupled with sexual reproduction in most cases, which can occur either through homothallism(self-fertilization) or heterothallism(intercrossing of +/-strains). The types of sexual reproduction have important ecological implications. The toxic and HAB-forming dino?agellate, Pheopolykrikoides hartmannii has been previously reported to be heterothallic. Here, we provide visual con?rmation of homothally of P. hartmannii and the ?rst detailed visual recording of cyst germination based on the observations of a clonal isolate from Jiaozhou Bay, China. To document the homothallism, we ?rst observed cell pairs in sexual mating, planozygotes with two longitudinal ?agella, and cysts with typical morphology as described previously from the clonal culture. We then germinated a single cyst, established a new clonal culture from one of the two daughter cells after the ?rst cell division of the germling(i.e. from the diploid germling to two haploid cells), and produced cysts again from the newly established clonal culture. For the observation of the germination time-series, we took micrographs and videos to show all germination processes, particularly with an interesting observation of the short amoeboid stage of the germling releasing from the archeopyle(～15 s), which was a landmark of the germination process and has not been reported elsewhere. This de?nitive evidence of homothallic sexuality and cyst production in P. hartmannii provides a new insight into the biology and ecology of the species, particularly a mechanism that may partly account for the population dynamics and ubiquitous distribution of the species.     

西宁城市居民家内外活动时间分配及影响因素——基于结构方程模型的分析

为深入挖掘居民不同类型活动的出行需求,进而制定更加精细化的交通政策,从活动分析法的视角出发,基于结构方程模型分析西宁城市居民家内外活动时间分配的机理及影响因素。结果表明:工作日,居民的通勤出行仍然是城市交通需要重点解决的问题。休息日,工作和通勤时间长的居民更倾向于在家内进行维持活动;而休息日不工作的居民更容易产生进行家外维持活动的出行需求。不同社会经济属性的居民对于家内外时间分配具有差异化特征。单位大院职住接近的特征对于减少居民通勤出行时间仍然具有积极作用,但维持性活动时间分配与其他类型社区居民已经没有显著差异。     

太平洋几种海面要素的升降趋势及其对海平面上升的影响

对ＣＯＡＤＳ资料中１９５０－１９９２年太平洋４°×８°方区的海水表层温度及海面气温、气压、风速序列数据，进行了时间序列分析。分析结果表明：太平洋海表气温和水温年上升率分别为０．００６３℃·ａ（－１）和０．００２６℃·ａ（－１）。两者的变化趋势在赤道太平洋都有一个年上升率高值区；而在东海以北的西北太平洋广大海区，都有一个年下降率的低值区。西太平洋海面气压呈上升趋势，东太平洋则相反。气温和水温的升降区域分布特征与风速分量增强和减弱的变化趋势有关。文章还指出，中国和日本沿岸今后几十年海平面变化趋势的预测模式，应以太平洋或全球海平面上升趋势的预测为基础，加上本海区海平面的局地变化订正。     

The buckling of GRP hemi-ellipsoidal dome shells under external hydrostatic pressure

The paper reports on a theoretical and an experimental investigation into six GRP hemi-ellipsoidal dome shells, which were tested to destruction under external hydrostatic pressure. All six domes were of oblate shape and of three different aspect ratios. Each aspect ratio consisted of two dome shells, one made from two layers of glass fibre and one made from three layers of glass fibre. The theoretical analysis was via the finite element method where a non-linear theory was used which allowed for both geometrical and material non-linearity. Comparison between theory and experiment was good.     

Size-fractionated phytoplankton standing stock and primary production in the Bohai Sea during late spring

During June 1997 cruise by R/V Science No.l, observations on temporal and spatialvariations of the size-fractionated phytoplankton standing stock and primary production were carried out in the Bohai Sea. The size-fractionated chlorophyll a (Chl a) and primary production, photosynthet-ically available radiation (PAR), as well as the related physico-oceanographic and zooplanktonic parameters were measured at five time-series observation stations representing sub-areas of the sea. Results obtained show that there were the marked features of spatial zonation of Chl a and primary production in the Bohai Sea. The values in the Laizhou Bay, the Liaodong Gulf and the Bohai Gulf were high and showed close relation with tidal fluctuations, i.e. high Chl a concentration occurred during high tide in the Laizhou Bay, and during low tide in the Liaodong Gulf and the Bohai Gulf. In the strait and the central region of the Bohai Sea, the values were relatively low and no relationship with tidal fluctuation could be foun     

Brief Account of Breakwater Construction in China

-The construction of breakwaters in China in more than 40 years since the founding of the People's Republic of China is reviewed. The construction of two main types of breakwater, upright breakwaters and rubble mound breakwaters, and the way in which they are built are expounded. Recommendations to improve the technology for future breakwater construction are presented.     

Period components in the monthly mean sea level variations in the Pacific Ocean

ＰｅｒｉｏｄｃｏｍｐｏｎｅｎｔｓｉｎｔｈｅｍｏｎｔｈｌｙｍｅａｎｓｅａｌｅｖｅｌｖａｒｉａｔｉｏｎｓｉｎｔｈｅＰａｃｉｆｉｃＯｃｅａｎ￥ＴｉａｎＳｕｚｈｅｎ；ＭａＪｉｒｕｉ；ＺｈｅｎｇＷｅｎｚｈｅｎ；ＣｈａｉＸｉｎｍｉｎａｎｄＺｈａｎｇＱｉｎ（Ｒｅｃ...     

罗非鱼椭圆囊电生理学研究

罗非鱼球状囊微音器电位是一个双峰波。峰频与刺激声频成２：１关系；在２０－８０Ｈｚ电位波形不规则。在３００－９００Ｈｚ电位波呈矩形。实验表明，球状囊不同区域对一一的声频敏感性不一样；延脑－脊髓连结处的诱发电位与球状囊微音器电位相似，但在１３０Ｈｚ电位呈单峰波；罗非鱼可闻频域在２０－９００Ｈｚ，最大敏感声频在１３０Ｈｚ，其相对敏感性为２３．０１ｄＢ；延脑－脊髓连接处诱发电位最大值在６３Ｈｚ和１３０Ｈｚ     

基于Hoek-Brown准则的节理岩体能量参数估算

岩体变形和破坏可以看作能量耗散和释放的过程.由于节理岩体结构复杂且难以开展室内试验,因此无法通过试验直接求取其能量学参数.基于Hoek-Brown准则和岩石能量理论,提出了节理岩体在临界状态能量学参数的估算方法.针对含贯通节理(或层面)岩体,通过修正岩块单轴抗压强度以体现贯通节理的方向效应.采用PFC3D分别模拟小尺寸岩样(Φ50mm×100mm)和大尺寸岩体(Φ2m×2m)的三轴压缩试验,通过岩石三轴试验结果拟合岩石数值模拟的细观参数并应用于节理岩体的模拟.根据节理岩体模拟得到应力应变曲线和能量流,验证了Hoek-Brown准则对节理岩体能量参数估算的合理性.     

PVD and DCM columns combined ground improvement for embankment construction

Acta Geotechnica - Prefabricated vertical drains (PVDs) and deep cement mixing (DCM) columns combined ground improvement with a vacuum pressure for embankment construction on soft clay deposits has...