关于山东省地热尾水回灌试验的应用研究

为解决地热水开采利用过程中造成诸如水位下降、热流体排放引起的热污染和化学污染等问题,通过开展地热尾水回灌试验研究,认为在开采深层的地下热水资源的同时,必须进行人工的回灌补源,山东省通过在德州、东营、济南等地开展地热尾水回灌试验工作,效果显著。     

1961—1987年与1988—2014年辽宁省气候区划界线变化及可能气候成因

基于同一区划方法、指标体系,使用1961—2014年辽宁省52站气象观测资料,分析辽宁省气温、气候区划指标、范围及界线的变动特征。结果表明：辽宁省年均气温在1988年发生一次突变,突变后气温开始显著上升;≥10 ℃积温日数比较显著地响应气温突变,而干燥指数、7月平均气温变化不显著。在空间分布上区划指标值均存在不同程度的变化。① 全省≥10 ℃积温日数均出现增加,但在中西部地区显著增加;② 在盘锦-抚顺一线以北（南）,气候总体呈不显著变湿（干）趋势;③ 7月平均气温呈缓慢上升趋势。区划范围及界线位置出现更加显著地变化：① 暖温带范围主要向北向东扩展,中温带向东收缩;② 半湿润区范围主要向北向西扩展,半干旱区向西北方向收缩,湿润区范围基本不变;③ Tb范围显著向北向东扩展,Ta范围向北向东收缩。在此基础上分析了气候格局变化的可能气候成因,发现突变后≥10 ℃积温日数期间500 hPa高度场增加与4月和10月东亚冬季风减弱,4—10月东北冷涡持续天数增加和7月500 hPa高度场增加,可能分别是温度带,Tb区、Ta区和半湿润区、半干旱区变化的原因。     

Effect of carbon and nitrogen ratio control on Artemia growth,water quality,biofloc microbial diversity under high salinity and zero-water exchange culture condition

Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species.The growth of heterotrophic microorganisms can be stimulated through adding extra carbon,which reduces the nitrogen level in the water and provides microbial protein to the animals.However,most of the studies and practical applications have been conducted in freshwater and marine environment.This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms.The effect of carbon supplementation on Artemia growth,water quality,and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange.The salinity of the culture medium was 100.A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen(C/N)ratio of 5,15,and 30(Su5,Sul5,and Su30),respectively.The culture without adding sucrose was used as a control.Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level.The results showed that sucrose supplementation at higher C/N ratio(15 and 30)significantly improved the Artemia survival,growth and water quality(P0.05).Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume(BFV)(P0.05).The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness,and shaped the microbial composition at genera level.This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.     

Impacts of Coastal Reclamation on Natural Wetlands in Large River Deltas in China

Little information is available on the impacts of coastal reclamation on wetland loss in large-river deltas at a regional scale.Using remote sensing data of coastal wetland and reclamation in four deltas in China from 1978 to 2014, we tracked their continuous area changes in four periods: 1978–1990, 1990–2000, 2000–2008, and 2008–2014. The areal relation between wetland loss and reclamation was quantified and used to identify coastal reclamation mode intensity coupled with another three indicators: reclamation rate,accretion rate and land-use intensity of coastal reclamation. The results showed that coastal reclamation driven by economic development reduced, or even reverse the original growth of delta which was determined by the offset between wetland acceleration rate and wetland loss rate. Generally, the area of reclamation showed a positive linear correlation with the area of wetland loss. The findings imply that human activities should control reclamation rate and intensity to alleviate total wetland loss and maintain wetland ’net gain’.Inappropriate coastal reclamation modes can magnify total wetland loss;therefore, coastal reclamation with a slow increment rate and low impervious surface percent is of great importance for sustainable development in future coastal management.     

条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。     

海水人工湿地系统脱氮效果与基质酶活性的相关性

利用海水人工湿地系统处理海水养殖外排水,分析了人工湿地对不同形态氮的净化效果,探讨了人工湿地表层基质酶活性变化及其对系统脱氮效果的影响。选取互花米草作为人工湿地植物,煤渣、珊瑚石和细砂作为人工湿地基质,实验期间连续进水,系统运行稳定。研究结果表明:海水人工湿地系统对氨氮(NH4-N)、亚硝态氮(NO2-N)、硝态氮(NO3-N)、总氮(TN)和可溶性有机氮(DON)去除效果显著,去除率分别为(99.6±0.7)%、(99.9±0.0)%、(98.2±2.0)%、(92.6±1.5)%和(86.1±4.8)%。人工湿地表层基质下行池脱氢酶、硝酸还原酶和脲酶的酶活性均高于上行池,下行池对污染物的去除效果更好。脱氢酶活性与海水人工湿地系统氨氮的去除有关;硝酸还原酶活性影响着海水人工湿地硝态氮的去除;脲酶活性与人工湿地总氮和硝态氮的去除存在明显相关趋势。下行池硝酸还原酶和脲酶的酶活性间具有显著相关性(r=0.76, P0.05)。人工湿地微生物种类丰富,下行池微生物多样性高于上行池,植物根部微生物多样性最高,提高了系统脱氮的效率。上述研究结果将有助于阐明海水人工湿地系统中不同形态氮的迁移转化机理。     

养殖裙带菜机械损伤防御应答的初步研究

以裙带菜(Undariapinnatifida(Harv.)Suringar)幼孢子体为材料,测定藻体对机械损伤的防御应答。结果显示:与对照组相比,机械损伤会诱导藻体内活性氧含量上升,以及抗氧化酶活性和抗氧化物含量的变化。当裙带菜受到机械损伤后,体内O2-和H2O2以及MDA等物质迅速积累,藻体的总抗氧化能力上升、CAT和POD活性在短时间内迅速升高、GSH和ASA迅速合成,从而在较短时间内控制活性氧以及MDA含量;与对照组相比,SOD活性仅在3h内高于对照组。该实验结果表明裙带菜幼孢子遭受机械损伤后的抗氧化系统会参与调节机体活性氧平衡,这种抗氧化机制的存在可以更快速、更节能、更有效地修复藻体所受损伤。     

深圳市浴场用海定级研究

海域分等定级是制定海域使用金征收标准和开展海域资产价格评估的前提和依据。借鉴《海域定级技术指引(试行)》,遵循资源禀赋差异、收益差异、节约利用和生态用海的原则选取海域自然条件和海域区位条件2个定级因素及海水质量、海洋自然灾害等10个评价因子,构建深圳市浴场用海定级的评价指标体系。采用综合指数评价法计算出207个评价单元的得分情况,通过聚类分析方法和变异系数(CV)来校核级别,最终将浴场用海的海域划分为Ⅰ级(22.22%)、Ⅱ级(33.82%)和Ⅲ级(43.96%)3个级别。研究成果为海洋管理部门制定浴场用海不同级别的基准价格提供了依据。     

航空重力垂直梯度探测潜艇方法研究

航空重力梯度测量属于被动探测,抗干扰能力强,如果能和其他探潜手段相配合将极大地提高航空搜潜的效率。针对航空重力梯度测量是否能够用于探测潜艇的问题,依据俄亥俄级弹道导弹核潜艇的结构特点,建立了适用于重力梯度计算的潜艇模型,分别给出了潜艇外壳、内部质量亏损产生的重力垂直梯度的计算方法,并对不同精度重力梯度仪可探测的潜艇重力垂直梯度值进行了计算,从航空反潜的角度对探测潜艇效果进行了分析。经计算表明,如航空重力梯度仪精度达到10~(-2) E,将具备一定的实际探潜效能;如精度达到10~(-4) E,反潜机搜索宽度可与现有航空磁性探潜相当。