东太平洋赤道附近海域茎柔鱼(Dosidicus gigas) 渔业生物学的初步研究

根据2011年东太平洋赤道附近海域探捕期间采集的1178尾茎柔鱼样本,进行了茎柔鱼的生物学特性研究。结果表明:茎柔鱼胴长范围为201—421mm,平均胴长为290mm,优势胴长为260—320mm;体重范围为200—2650g,平均体重为1000g,优势体重为600—1000g;胴长与体重关系呈幂指数关系;雌、雄性比为2.59:1;性腺成熟度以I、Ⅱ期为主;缠卵腺长和缠卵腺重随着性腺的成熟而逐步增大;雌性初次性成熟胴长为397.2mm;摄食等级以0—1级为主;与其它海域相比,该海域茎柔鱼个体差异明显,主要为小型群体。     

基于GIS的莱州湾南岸土壤盐渍化特征分析研究

运用地统计学,结合GIS技术研究了莱州湾南岸地区土壤含盐量空间变异性.结果表明:土壤含盐量的变异系数除表层和底层属强变异性外,其它均属中等变异强度.整个研究区土壤含盐量较高,盐渍土所占面积较大;除10～20 cm和80～100 cm土层土壤含盐量分布符合球状模型外,其它各层均符合指数模型.研究区不同土层土壤盐分含量具有明显的空间变异性,除60～80 cm和80～100 cm土层土壤含盐量具有强空间相关外性,其它各层均具有中等空间相关性.该研究为莱州湾南岸地区区域规划、农业生产和生态环境维护提供理论依据.     

长江口外表层沉积物黏土矿物分布特征（英文）

依据对长江口外表层沉积物187个站位沉积物样品的黏土矿物含量分析,研究了长江口外表层沉积物黏土矿物的组合特征和分布规律。长江口外表层沉积物伊利石为优势矿物,平均含量为60.3%;次之为蒙脱石,平均为14.9%;高龄石平均含量13.5%,绿泥石平均含量为12.3%。黏土矿物的组合类型以伊利石-蒙脱石-高岭石-绿泥石型为主,伊利石-高岭石-绿泥石-蒙脱石型次之;长江口外表层沉积物黏土矿物主要为陆源成因,物质主要来源于黄河和长江的供给。Q型聚类分析显示,现代黄河物质及老黄河物质主要沉积于研究区东北部北纬29°30′以北地区,长江物质主要在研究区西部及中部区域沉积。     

南海与厦门湾海水中210Po和210Pb的粒级分布

对南海和厦门湾表层水中210Po和210Pb的粒级分布特征进行了研究.结果表明,南海海水中210Po和210Pb在不同粒级颗粒物中的体积比活度高低顺序为:(＜0.4μm)＞(0.4～2.0 μm)＞(2.0～10 μm)＞(＞10μm).210Po、210Pb主要存在于＜2.0 μm粒级颗粒物中.厦门湾210Po体积比活度高低顺序为:(2.0～10 μm)＞(＜1.2μm)＞(＞10μm)＞(1.2～2.0 μm),210Po主要存在于＞2.0μm粒级颗粒物中.南海不同粒级颗粒物中210Pb的质量比活度差异不大,0.4～2.0 μm和2.0～10 μm粒级210Po质量比活度约为10μm粒级210Po比活度的3倍.厦门湾210Po的质量比活度比南海相应粒级210Po低约1个数量级,且各粒级间差异不大,表明不同粒径颗粒物在210Po和210Pb清除过程中的作用不同.210Po和210Pb的固-液分配系数(Kd)及分馏因子(α)进一步证实南海0.4～2.0 μm粒级颗粒物对210Po的清除能力比其它粒级颗粒物更强,而＞10μm粒级颗粒物对210Pb的清除能力比其它粒级颗粒物更强.不同粒级颗粒物中210Po与210Pb之间的不平衡状况揭示了不同粒级颗粒物不同的来源,为210Po/210Pb不平衡示踪颗粒物来源提供了依据.     

文蛤抗癌多肽对N-乙酰-β-D氨基葡萄糖苷酶活力的影响

从文蛤体中分离纯化得到了抗癌多肽,研究该多肽对N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)活力的影响,结果显示文蛤抗癌多肽对该酶有明显的抑制作用.在本文中,我们将深入地研究其抑制作用机理和抑制效应类型,结果表明:随着文蛤多肽加入量的增大,该酶活力呈指数下降,测得导致酶活力下降50%的抑制剂浓度(IC50)为112.9 μg/cm3,该多肽对酶的抑制作用是一种可逆过程,抑制机理表现为混合型抑制类型,对游离酶(E)的抑制常数(KI)和酶-底物络合物的抑制常数(KIS)分别为77.03和697.44μg/cm3,说明文蛤抗癌多肽与游离酶的结合导致酶活力的丧失,明显的强于对酶-底物络合物的抑制效应,底物的存在对该酶被文蛤抗癌多肽抑制作用起了保护作用.     

对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

A modified analytical method for the shipboard determination of nanomolar concentrations of orthophosphate in seawater

A method for the determination of nanomolar concentrations of orthophosphate in oligotrophic seawater developed by Liang et al. (2007) has been modified to make it fully feasible for shipboard application and for faster sample throughput with minimized sample volume. The technique is based on the flow injection method with solid phase extraction on a Sep-Pak C18 cartridge and colorimetric detector. The Schlieren effect was minimized by rinsing the cartridge sequentially with 5 mL water and 2 mL 95% ethanol solution. With three micro pumps in parallel, savings of up to 80% in amount of reagents and 25% volume of seawater samples could be achieved in comparison to the previous method. Variation of stopped flow time and sample loading time gave 3 different standard curves, which corresponded to 3 linear ranges within 3.4 and 515 nM. The modified method permits the analysis of samples over a wide range of concentrations, and has been successfully applied to shipboard determination of trace orthophosphate in more than 200 seawater samples during a one-month cruise in the South China Sea. For seawater at concentrations of 20.6, 82.5, 206.2 nM orthophosphate, the relative standard deviations (RSD) (n = 6), determined daily for 6 days on board ship were 4.45%, 4.73% and 6.75%, respectively. Five seawater samples collected in the Station SEATS (South East Asia Time Series Station at 18°N, 116°E) were analyzed using the present method both on board and in a land-based laboratory, as well as with the magnesium hydroxide-induced coprecipitation (MAGIC) method, and showed no significant difference according to the statistical t-test.     

Dissolved inorganic carbon (DIC) and its carbon isotopic composition in sediment pore waters from the Shenhu area,northern South China Sea

The Shenhu area is one of the most favorable places for the occurrence of gas hydrates in the northern continental slope of the South China Sea. Pore water samples were collected in two piston cores (SH-A and SH-B) from this area, and the concentrations of sulfate and dissolved inorganic carbon (DIC) and its carbon isotopic composition were measured. The data revealed large DIC variations and very negative δ ¹³C-DIC values. Two reaction zones, 0–3 mbsf and below 3 mbsf, are identified in the sediment system. At site SH-A, the upper zone (0–3 mbsf) shows relatively constant sulfate and DIC concentrations and δ ¹³C-DIC values, possibly due to bioturbation and fluid advection. The lower zone (below 3 mbsf) displays good linear gradients for sulfate and DIC concentrations, and δ ¹³C-DIC values. At site SH-B, both zones show linear gradients, but the decreasing gradients for δ ¹³C-DIC and SO₄ ²⁻ in the lower zone below 3 mbsf are greater than those from the upper zone, 0–3 mbsf. The calculated sulfate-methane interface (SMI) depths of the two cores are 10.0 m and 11.1 m, respectively. The depth profiles of both DIC and δ ¹³C-DIC showed similar characteristics as those in other gas hydrate locations in the world oceans, such as the Blake Ridge. Overall, our results indicate an anaerobic methane oxidation (AMO) process in the sediments with large methane flux from depth in the studied area, which might be linked to the formation of gas hydrates in this area.     

近40年来珠江口的海平面变化

利用大万山附近1°×1°经纬度网格的卫星高度计资料(1993—2006),计算出珠江口绝对海平面的上升速率为0·30±0·05cm/a,与由卫星高度计得出的全球平均海平面的上升速率一致。珠江口各验潮站近40年的潮位变化趋势分析表明,珠江口海平面正加速上升,为全球气候变暖所致;珠江口海平面与全球温度变化和ENSO活动密切相关,一般在ENSO年海平面相对较低。以IPCC有关全球温度上升幅度的预报值和海平面与全球温度变化的关系为依据,预计到2030和2050年珠江口绝对海平面将分别上升6～14和9～21cm,若考虑地面沉降以及波动值,珠江口部分岸段相对海平面将可能分别上升30和50cm。     

天然气水合物的声学探测模拟实验

在人工岩心中进行天然气水合物的生成和分解实验,同时获取了体系的温度、压力、声学特性(Vp和Vs、幅度和频率)及含水量等参数。经研究发现,温压法、超声探测法和TDR探测法都能灵敏探测沉积物中天然气水合物的形成和分解过程。分析认为,本次实验中水合物形成速率过快,只能宏观研究水合物对沉积物声学特性的影响,建议采用长岩心进一步研究沉积物中水合物的声学特性。