Pseudo-cryptic species Arenicola defodiens and Arenicola marina (Polychaeta: Arenicolidae) in Wadden Sea,North Sea and Skagerrak: Morphological and molecular variation

The polychaete Arenicola defodiens, or black lug, was recently described as a morphologically highly similar species alongside the blow lug Arenicola marina. So far it was only known from the British Isles. A double spawning peak was observed earlier in lugworms of the western Wadden Sea. Here, we test the hypothesis that the two spawning peaks represent the two species in sympatry. This hypothesis is refuted on the basis of both morphological and mitochondrial DNA data; both spawning peaks are attributed to A. marina. In spite of this, we confirm, on the basis of new collections as well as re-examination of museum collections, the presence of A. defodiens in the western Wadden Sea, North Sea and also the Skagerrak; its distribution is restricted to habitats which are either submerged or have short emersion times, have relatively coarse sediments and high and stable salinities. Sympatry is common. The species differ strongly in the mitochondrial DNA fragment examined; the observed 14% uncorrected minimum difference amounts to an estimated 33–63 million years since sequence divergence. The amount of intraspecific molecular variation is larger for A. defodiens than for A. marina. This is evidence to suggest that A. marina may have undergone more recent and/or more severe population size bottlenecks.     

Open-system chemical behavior in deep Wilcox Group mudstones,Texas Gulf Coast,USA

Wilcox Group mudstones have been mechanically and geochemically transformed over a temperature range of 20–200 °C. Our research controlled for provenance and age by sampling from five wells, parallel to the paleodepositional axis, all within the Houston delta system. Across the sampled depths, mudstone porosity has been reduced from ∼25 to <10% and bulk mineralogical change as documented by quantitative X-ray diffraction includes decreases in quartz, K-feldspar and kaolinite content whereas illite + illite − smectite, chlorite, and plagioclase increase. These mineral transformations transfer elements at a scale of less than 1 mm from one mineralogical form to another, however, X-ray fluorescence data suggest that among major elements only Al₂O₃ and TiO₂ are fully conserved within the system (trace-element ZrO₂ is also conserved). K₂O has been added to and SiO₂ released from the Wilcox Group mudstones. Cathodoluminescence and secondary electron imaging did not find this SiO₂ locally precipitated. We, therefore, document an open-system geochemical behavior.     

Equatorial Pacific gravity lineaments: interpretations with basement topography along seismic reflection lines

The central equatorial Pacific is interesting for studying clues to upper mantle processes, as the region lacks complicating effects of continental remnants or major volcanic plateaus. In particular, the most recently produced maps of the free-air gravity field from satellite altimetry show in greater detail the previously reported lineaments west of the East Pacific Rise (EPR) that are aligned with plate motion over the mantle and originally suggested to have formed from mantle convection rolls. In contrast, the gravity field 600 km or farther west of the EPR reveals lineaments with varied orientations. Some are also parallel with plate motion over the mantle but others are sub-parallel with fracture zones or have other orientations. This region is covered by pelagic sediments reaching ~?500–600 m thickness so bathymetry is not so useful for seeking evidence for plate deformation across the lineaments. We instead use depth to basement from three seismic reflection cruises. In some segments of these seismic data crossing the lineaments, we find that the co-variation between gravity and basement depth is roughly compatible with typical densities of basement rocks (basalt, gabbro or mantle), as expected for some explanations for the lineaments (e.g., mantle convection rolls, viscous asthenospheric inter-fingering or extensional deformation). However, some other lineaments are associated with major changes in basement depth with only subtle changes in the gravity field, suggesting topography that is locally supported by varied crustal thickness. Overall, the multiple gravity lineament orientations suggest that they have multiple origins. In particular, we propose that a further asthenospheric inter-fingering instability mechanism could occur from pressure variations in the asthenosphere arising from regional topography and such a mechanism may explain some obliquely oriented gravity lineaments that have no other obvious origin.     

Evaluation of Inference Adequacy in Cumulative Logistic Regression Models: An Empirical Validation of ISWRidge Relationships

Internal solitary wave propagation over a submarine ridge results in energy dissipation, in which the hydrodynamic interaction between a wave and ridge affects marine environment. This study analyzes the effects of ridge height and potential energy during wave-ridge interaction with a binary and cumulative logistic regression model. In testing the Global Null Hypothesis, all values are p 〈0.001, with three statistical methods, such as Likelihood Ratio, Score, and Wald. While comparing with two kinds of models, tests values obtained by cumulative logistic regression models are better than those by binary logistic regression models. Although this study employed cumulative logistic regression model, three probability functions p^1, p^2 and p^3, are utilized for investigating the weighted influence of factors on wave reflection. Deviance and Pearson tests are applied to cheek the goodness-of-fit of the proposed model. The analytical results demonstrated that both ridge height （X1 ） and potential energy （X2 ） significantly impact （p 〈 0. 0001 ） the amplitude-based refleeted rate; the P-values for the deviance and Pearson are all 〉 0.05 （0.2839, 0.3438, respectively）. That is, the goodness-of-fit between ridge height （ X1 ） and potential energy （X2） can further predict parameters under the scenario of the best parsimonious model. Investigation of 6 predictive powers （ R2, Max-rescaled R^2, Sorners＇ D, Gamma, Tau-a, and c, respectively） indicate that these predictive estimates of the proposed model have better predictive ability than ridge height alone, and are very similar to the interaction of ridge height and potential energy. It can be concluded that the goodness-of-fit and prediction ability of the cumulative logistic regression model are better than that of the binary logistic regression model.     

Ex situ cultivation of Aplysinaaerophoba close to in situ conditions: ecological, biochemical and histological aspects

Sponges provide the largest number of biologically active natural products known from the marine environment and continue to be a very well studied phylum of marine fauna. The Mediterranean sponge Aplysina aerophoba accumulates brominated isoxazoline alkaloids such as Aplysinamisin-1, Aerophobin-2, Isofistularin-3 and the biotransformation product Aeroplysinin-1, which possesses, for example, antibiotic and cytotoxic properties. Until now, it is still being discussed which organisms – the sponge itself or associated microorganisms – are responsible for metabolite production. For cultivating Aplysina individuals under ex situ conditions, we surveyed relevant ecological factors in situ and controlled them in our aquarium system. We maintained A. aerophoba for more than 9 months and analysed changes of metabolite content and composition, microbial association as well as morphology in situ and ex situ under different light exposure. Although sponges showed slight reduction during maintenance, ex situ cultivation similar to in situ conditions provides a promising method to keep sponges and obtain their bioactive metabolites.     

Shallow Drilling of Seafloor Hydrothermal Systems Using the BGS Rockdrill: Conical Seamount (New Ireland Fore-Arc) and PACMANUS (Eastern Manus Basin), Papua New Guinea

ABSTRACT

From September to October 2002, shallow drilling, using the submersible (5 m) Rockdrill of the British Geological Survey and the German R/V Sonne revealed critical information on the subsurface nature of two distinct hydrothermal systems in the New Ireland fore-arc and the Manus Basin of Papua New Guinea. Drilling at Conical Seamount significantly extends the known surface extent of the previously discovered vein-style gold mineralization (up to 230 g/t Au) at this site. Drilling the conventional PACMANUS volcanic-hosted massive sulfide deposit recovered complexly textured massive sulfide with spectacular concentrations of gold in several core sections including 0.5 m @ 28 g/t Au, 0.35 m @ 30 g/t Au, and 0.20 m @ 57 g/t Au. Shallow drilling is a fast and cost efficient method that bridges the gap between surface sampling and deep (ODP) drilling and will become a standard practice in the future study of seafloor hydrothermal systems and massive sulfide deposits.     

Microsporidan parasite Thelohania contejeani Henneguy from New Zealand freshwater crayfish

The sporozoan Thelohania contejeani Henneguy (Microsporida: Nosematidae) is recorded from Paranephrops zealandicus (White) for the first time. It is an intracellular parasite invading musculature throughout the crayfish, causing a gradual decrease in locomotor activity, and it may eventually cause the death of the host. Infected crayfish were found in the northern part of Leith Stream, Dunedin, where the infection rate reached a maximum of 20%. This parasite has formerly been recorded only from Northern Europe.     

Reproductive cues in Panulirus ornatus

Two experiments were performed to assess the effect of photoperiod and temperature on spawning of Panulirus ornatus. In experiment 1, sexually mature lobsters taken from the wild during summer were held at one of two photoperiods, winter (13 Light: 11 Dark) and summer (14.5 Light:9.5 Dark). Additionally, lobsters were also exposed to either summer (29°C) or winter (24°C) average water temperatures. Spawning was significantly greater when animals were exposed to summer photoperiod than to winter photoperiod, irrespective of temperature. Although a higher percentage of lobsters spawned when placed under a higher temperature, this trend was not statistically significant. In experiment 2, sexually mature lobsters were taken from the wild during winter and exposed to the same two photoperiods as in experiment 1, at a summer equivalent temperature of 29°C. Breeding started earlier and was more successful at the summer photoperiod. Time to first breeding was 17 weeks after exposure to summer photoperiod, compared with less than 1 week in experiment 1, and did not occur until individuals had moulted. Moulting occurred in 81% of lobsters, primarily after an increase in temperature to 29°C. The time between moulting and mating was varied and there was no significant difference in moult frequency between the two experimental photoperiods. After the lobsters had moulted, breeding success was reached earlier if photoperiod was lengthened. Results suggest photoperiod is the primary cue for the onset of gonad maturity and mating activity, with temperature playing a less important role. Physiological rest and possibly a moult may be required between breeding seasons before spawning can occur. Furthermore, temperature may be an important cue for pre‐reproduction moulting.     

Uptake of plutonium from seawater and sediment by a marine polychaete worm

The results of the uptake of plutonium 239 from sediment by a marine polychaete wormNereis diversicolor is reported in the present paper. A comparison is made of the relative importance of two possible uptake pathways, sediment and seawater by which this worm may obtain its plutonium body-burden. Under the laboratory conditions used for the comparison of those two routes it would appear thatNereis obtains greater than 98% of its body activity from seawater.