Differential gene expression in mummichogs (Fundulus heteroclitus) following treatment with pyrene: comparison to a creosote contaminated site

Mummichogs, Fundulus heteroclitus, an estuarine fish with a relatively small home range found along the eastern coast of the United States are well-suited to monitoring contaminant effects, including those of polycyclic aromatic hydrocarbons (PAHs). One of the common PAHs in estuaries is pyrene. We report here on efforts to develop multiple biomarkers of pyrene exposure in this species. Adult male mummichogs were exposed in the laboratory to the weak aryl hydrocarbon receptor (AhR) agonist pyrene at 0, 30, or 50 microg/L in 7-day static renewal exposures. The RNA was extracted from livers and alterations in mRNA expression were assessed by subtractive hybridization and differential display in order to produce multiple biomarkers of pyrene exposure. Genes demonstrating differential expression were confirmed by quantitative-PCR (Q-PCR) and include cytochrome P-450 1A (CYP1A), a putative hepatocyte growth factor activator, a X-ray inducible retrotransposon, and several expressed sequenced tags (ESTs). Some of these genes represent new biomarkers of pyrene exposure and potential biomarkers of PAH exposure. Therefore, similar changes were investigated at a Superfund site in Charleston, SC. Mummichogs from a creosote contaminated site and from a reference site (North Inlet National Estuarine Research Reserve near Georgetown, SC) were trapped, RNA extracted from the livers, and Q-PCR performed. Many of the genes differentially expressed following pyrene exposure were not altered at the creosote contaminated site in comparison to the reference site. However, CYP1A and an EST were induced. CYP1A induction at Diesel Creek indicates that this population of fish does not demonstrate refractory CYP1A phenotypes observed at several sites with high levels of AhR agonists. Ultimately, we anticipate that the use of multiple biomarkers of PAH exposure will provide useful information on the potential effects of toxicants.     

The viviparous blenny (Zoarces viviparus) as a bioindicator of contaminant exposure: application of biomarkers of apoptosis and DNA damage

The viviparous blenny (Zoarces viviparus), also known as eelpout, is considered a suitable biomonitor for use in European estuarine and coastal waters. We have previously shown that Z. viviparus from the industrialised Tyne estuary (UK) display certain histopathologies (e.g., ovotestis and hepatic nuclear and cellular pleomorphism), which are associated with contaminant exposure [Mar. Environ. Res. 55 (2003) 137]. Furthermore, the prevalence of these pathologies was higher than in fish collected from a less contaminated reference site (the Alde estuary, UK). Here, tissue samples were collected from Z. viviparus from the Tyne and Alde estuaries (in the spring and autumn) and analysed for DNA adducts using the (32)P-postlabelling assay and for histopathology. Z. viviparus caught from the Tyne during the spring (56.4+/-18.5 adducts per 10(8) nucleotides) and autumn (63.1+/-7.5 adducts per 10(8) nucleotides) exhibited patterns of DNA adducts which indicated exposure to a complex mixture of genotoxins. In contrast, levels of DNA adducts in Z. viviparus from Alde estuary were significantly lower during both sampling periods. Histopathological changes to the gonads included intersex (ovotestis) and germ cell apoptosis (detected via the TUNEL assay) in male fish captured from the Tyne estuary. Further studies on the use of germ cell apoptotic rate at different estuarine sites are required if it is to be used as a reproductive biomarker of contaminant exposure in male fish.     

Ten new very low‐mass close binaries resolved in the visible

We present preliminary results from the first part of the LuckyCam late M‐dwarf binarity survey. We survey a sample of 48 nearby (< 40 pc) and red (M5–M9) stars with the novel high angular resolution visible light imaging technique Lucky Imaging, in only 8 hours of 2.5m telescope time. We discover 10 new binaries; although the survey is sensitive to brown dwarf companions none are detected. The orbital radius distribution of the newly discovered binaries broadly matches that of previous detections by other groups, although we do discover one wide binary at ∼40 AU. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

Photometric correction of Mercury's global color mosaic

During the third flyby of Mercury by the MESSENGER spacecraft, a dedicated disk-integrated photometric sequence was acquired with the wide-angle multispectral camera to observe Mercury's global photometric behavior in 11 spectral filters over as broad a range of phase angle as possible within the geometric constraints of the flyby. Extraction of disk-integrated measurements from images acquired during this sequence required careful accounting for scattered light and residual background effects. The photometric model fit to these measurements is shown to fit observed radiances at phase angles below 110°, possibly except where both solar incidence and emission angles are high (>70°). The complexity of the scattered light at wavelengths greater than 828 nm contributes to a less accurate photometric correction at these wavelengths. The model is used to correct the global imaging data set acquired at a variety of geometries to a common geometry of incidence angle=30°, emission angle=0°, and phase angle=30°, yielding a relatively seamless mosaic. The results here will be used to correct image mosaics of Mercury acquired in orbit.     

The global distribution of pyroclastic deposits on Mercury: The view from MESSENGER flybys 1–3

We present a global survey of candidate pyroclastic deposits on Mercury, derived from images obtained during MESSENGER flybys 1–3 that provided near-global coverage at resolutions between 5 and 0.5 km/pixel. Thirty-five deposits were identified and characterized and are located principally on the floors of craters, along rims of craters, and along the edge of the Caloris basin. Deposits are commonly centered on rimless, often irregularly shaped pits, mostly between 5 and 45 km in diameter. The deposits identified are generally similar in morphology and absolute reflectance to lunar pyroclastic deposits. Spectrally the deposits appear brighter and redder than background Mercury terrain. On the basis of the available coverage, the candidate pyroclastic deposits appear to be essentially globally distributed. The diameters of the deposits, when mapped to lunar gravity conditions, are larger than their lunar counterparts, implying that more abundant volatiles were present during the typical eruptive process than on the Moon. These observations indicate that if these deposits resulted from hawaiian-style eruptions, the volatile contents required would be between ～1600 and 16,000 ppm CO or an equivalent value of H₂O, CO₂, SO₂, or H₂S (for a more oxidizing interior), or N₂, S₂, CS₂, S₂Cl, Cl, Cl₂, or COS (for a more reducing interior). These abundances are much greater than those predicted by existing models for Mercury's formation. An apparent lack of small deposits, compared with the Moon, may be due to resolution effects, a topic that can be further assessed during the orbital phase of the MESSENGER mission. These results provide a framework within which orbital observations by MESSENGER and the future BepiColombo mission can be analyzed.     

Laboratory impacts into dry and wet sandstone with and without an overlying water layer: Implications for scaling laws and projectile survivability

Abstract— Scaling laws describing crater dimensions are defined in terms of projectile velocity and mass, densities of the materials involved, strength of the target, and the local gravity. Here, the additional importance of target porosity and saturation, and an overlying water layer, are considered through 15 laboratory impacts of 1 mm diameter stainless steel projectiles at 5 km s^?1 into a) an initially uncharacterized sandstone (porosity ?17%) and b) Coconino Sandstone (porosity ?23%). The higher‐porosity dry sandstone allows a crater to form with a larger diameter but smaller depth than in the lower‐porosity dry sandstone. Furthermore, for both porosities, a greater volume of material is excavated from a wet target than a dry target (by 27–30%). Comparison of our results with Pi‐scaling (dimensionless ratios of key parameters characterizing cratering data over a range of scales) suggests that porosity is important for scaling laws given that the new data lie significantly beneath the current fit for ice and rock targets on a π_v versus π₃ plot (π_v gives cratering efficiency and π₃ the influence of target strength). An overlying water layer results in a reduction of crater dimensions, with larger craters produced in the saturated targets compared to unsaturated targets. A water depth of approximately 12 times the projectile diameter is required before craters are no longer observed in the targets. Previous experimental studies have shown that this ratio varies between 10 and 20 (Gault and Sonett 1982). In our experiments ?25% of the original projectile mass survives the impact.     

Lessons from co-impacts assessment under the Mitigation Action Plans and Scenarios (MAPS) Programme

The MAPS Programme has been active in Brazil, Chile, Colombia and Peru in supporting the development of an evidence base to inform the processes involved in climate mitigation policy-making. The programme combines detailed quantitative analysis with an extensive stakeholder engagement process to provide policy and decision makers with the information required for long-term climate mitigation planning.

In recognition of the critical need to consider the developmental context and agenda in climate policy-making, the projects undertaken in these countries have experimented with various types of assessments of developmental impacts at both the macro and micro levels. These impacts have been collectively termed co-impacts to reflect the fact that these may be either positive or negative. In this article the value and challenges associated with co-impacts work for climate mitigation policy are considered. The value of including co-impacts analysis in the MAPS processes included increased stakeholder buy-in, making the case for mitigation action, informing INDCs and the prioritization of particular mitigation actions. Challenges include those associated with obtaining locally relevant data, the selection and operation of appropriate macro-economic models and analytical approaches and working with multidisciplinary teams.

The article concludes by making some suggestions to optimize co-impacts work, through reference to other bodies of literature. The authors highlight that this article is situated within a dominant approach to climate mitigation policy work in developing countries, which implicitly places the non-climate benefits of any action as secondary to the primary benefit of climate change mitigation and constructs an artificial separation of policy formulation and implementation. Practitioners are encouraged to reflect on the implications of these considerations in future efforts.

Policy relevance

One of the primary challenges to advancing climate mitigation policy in developing countries is that of perceived relevance: near-term development priorities are overwhelming. Identifying, understanding and engaging with the co-impacts of mitigation actions has emerged as one way of addressing this challenge. Although still at an experimental phase, experience with co-impacts analysis in the MAPS countries provides some useful lessons in how to develop this area of work.