鲨肝刺激物质类似物基因在大肠杆菌中的表达与产物活性分析

构建了鲨肝刺激物质类似物基因片段的原核表达载体质粒 (pET-sHSS) ,转化大肠杆菌后通过IPTG诱导获得原核表达产物 ,并利用凝胶层析方法对表达产物进行了分离纯化 ;进一步利用MTT比色法研究了该重组产物对肝癌细胞株SMMC -7721的增殖影响。结果表明 ,在1mmol/L的IPTG的诱导下 ,鲨肝刺激物质类似物基因片段在大肠杆菌BL21菌株中获得表达 ,表达产物的相对分子质量大小约为17500u,占菌体可溶性蛋白总量的38%左右 ;纯化后的产物在低浓度下 (<50ng/L)具有明显刺激SMMC7721细胞株增殖的活性 ,高浓度下 (>100ng/L)则明显抑制该肿瘤细胞株的生长 ,与天然鲨肝刺激物质的生物活性类似     

Study on water saturation computation models in complex pore volcanic reservoir

Determination of water saturation is important for reservoir evaluation.When complex pore structures such as fracture and cavity are present in reservoir,Archie equation is no longer suitable.According to different models of pore structure division,the authors studied water saturation computation models.The results show that dual porosity system is divided into four models.The first model is based on dual laterolog,the second is Dual Porosity Ⅰ,the third is Dual Porosity Ⅱ,and the last one is based on the conductive pore.Besides,the triple porosity system is triple porosity model.Compute water saturation was using all the above five models in volcanic reservoir in Songnan gas field.The triple porosity system is the most suitable model for water saturation computation in complex pore structure volcanic reservoir.     

汉江上游谷地与渭河谷地黄土化学风化程度比较

比较汉江上游谷地及渭河谷地典型黄土剖面的元素组成、化学风化强度及常量元素迁移特征,揭示秦岭南北两侧黄土-古土壤剖面的成壤强度及其所指示的环境演变特征,两者差异及共性如下：① 两剖面化学组成均以SiO₂、Al₂O₃和Fe₂O₃为主,元素组成均一且高度混合,与上部陆地壳（UCC） 的化学成分十分接近,该些证据均指示两区域黄土是来源广泛并经过充分混合的风尘堆积产物。② 据CIA 值可判定MTS 及YHC 剖面均经历了中等风化作用,比较两剖面CIW值、A-CN-K三角图投点特征及元素迁移率知,汉江上游谷地的黄土-古土壤序列经历的化学风化作用更强,Ca 及Na 元素的丢失率更高,Al、Na、Mg、Si 等常量元素的迁出率更大。③ 依据Fe、Na迁移率的全剖面变化曲线知,汉江上游谷地与渭河谷地自全新世以来经历了相同的气候演变阶段,均记录了6000-5000a BP的干冷气候事件。     

内蒙古敖兰敖日格一带侵入岩地球化学数据的统计分析与地质解释

采用数理统计方法,研究了内蒙古科右中旗敖兰敖日格一带出露的侵入岩的岩石地球化学特征.选用地质因子分析等统计方法,分析了解体后的敖兰敖日格岩体、杜尔基岩体中各侵入岩填图单位的常量元素、区域主要成矿元素等地球化学数据,总结了侵入岩的岩石地球化学特征由老到新的演化趋势及变化规律,探讨了各主要岩体与区域成矿的相关性,为区域地质找矿提供依据.     

济宁市翟村铁矿小体重值与磁性铁品位的回归分析

利用济宁市翟村铁矿勘查过程中取得的体重测试值与磁性铁品位数据进行了回归分析,得出回归方程为y=2.74+0.026x,经显著性、精度检验并结合实际实践,证明该一元回归方程具有显著性,预测精度较高。可以用该方程预测翟村铁矿区小体重值。     

Early warning research on salt-alkalization desertification in western Songnen Plain

Desertification is an environmental issue in the world. The salt-alkalization desertification land area formed by both primary and secondary salt-alkalization has extended in a large scale, which has become a significant eco-environmental problem. Based on the characteristics of eco-environment and the situation of desertification in western Songnen plain, this paper reports the analyzes of its formation in background and cause. An early warning system on the salt-alkalization desertification is established and the GIS technology is used to abstract the information of desertification evaluation index. Supported by the integrated technology of the GIS and ANN, the orientation and quantitative result of desertification are gained, which is helpful to the eco-environment protection and resource development in western Songnen Plain.     

东南极格罗夫山地区夏季的天气特征

利用中国南极科学考察队 1 998年 1 2月至 1 999年 1月在东南极格罗夫山地区地质考察期间所获得的天气资料 ,对该地区的温度、雪面温度、风速、风向等天气要素进行了分析。结果表明 :夏季格罗夫地区温度日变化和盛行风向与中山站相类似 ,但温度日较差和强风频率比中山站大。当受来自北方暖湿气流影响时 ,常出现降雪天气 ;在东风气流控制下 ,天气以晴为主     

C6H水平井三维轨迹优选与控制技术

渤海北部LD油田在井网加密、油田大规模综合布置新钻调整井时,存在新钻的25口井与老井井网交叉、井眼轨迹立体三维缠绕问题,其中C6H水平井是此类问题的典型,同时C6H井还需要在可钻性极差的馆陶组底砾岩地层定向造斜。针对上述问题,通过C6H井3种轨迹方案对比、分析,优选出最为有利的方案,结合螺杆马达和旋转导向工具性能分析,最后采用螺杆马达 水力震荡器 微偏心稳定器等钻具组合新方式,顺利实施了C6H井。     

THE ACTIVITY OF WESTERN LISHAN FAULT SINCE THE LATE PLEISTOCENE

Along the northern piedmont of Mt. Lishan, the characteristics and locations of the active normal Lishan fault in west of Huaqing Pool provide important evidences for determining the seismotectonic environment, seismic stability evaluation of engineering in the eastern Weihe Basin. After reviewing the results from high-density resistivity method, seismic profile data, geological drillhole section and trenching in west of the Huaqing Pool, it is found that the strike of western normal Lishan Fault changes from EW direction at the eastern part to the direction of N60°W, and the fault consists of two branches, dipping NE with a high dip angle of~75°. The artificial shallow seismic profile data reveals that the attitude of strata near Lishan Fault mainly dips to south, which is presumed to be related to the southward tilt movement of Mt. Lishan since the Cenozoic. The section of geological drillhole reveals that since the late middle Pleistocene, the displacement of the paleo-soil layer S₂ is about 10m. And the maximum displacement of western Lishan Fault recorded in the paleo-soil layer S₁ reaches 7.8m since the late Pleistocene. In addition, evidences from trench profile show that the western Lishan Fault was active at least 3 times since Malan loess deposition with ¹⁴ C dating age(32 170±530)Cal a BP. The multiple activities of the Lishan Fault result in a total displacement about 3.0m in the Malan loess layer L₁. The latest activity of the western Lishan Fault produced a displacement of about 0.9m in the early Holocene loess layer L₀((8 630±20)Cal a BP)and caused obvious tensile cracks in the Holocene dark leoss layer S₀((4 390±20)Cal a BP). Briefly, we have obtained a vertical movement rate of about 0.11~0.19mm/a since the Holocene((8 630±20)Cal a BP)in the western extension of the Lishan Fault, the recurrence interval of earthquakes on the fault is about(10.7±0.5)ka, and the co-seismic surface rupture in a single event is inferred to be about 0.9m.     

不同源降水数据在天山南坡阿克苏河流域适用性分析

降水是水热循环以及气候变化研究的重要环节,降水资料的准确与否直接影响流域尺度的水文过程研究。本文基于2000-2015年天山南坡阿克苏河流域气象站点观测降水数据,对比分析了Tropical Rainfall Measuring Mission（TRMM）降水数据集和Global Land Data Assimilation System（GLDAS）两种具有代表性的降水格网数据集在阿克苏河流域的适用性。结果表明：TRMM3B43数据在阿克苏河流域的整体表现优于GLDAS-2数据。两种数据的精度在月尺度上表现最优,相关系数分别为0.938和0.901,通过了0.01的显著性检验;在季节尺度,TRMM3B43数据各季节与站点插值的拟合度要优于GLDAS-2数据,但二者均呈现出高估冷季降水而低估暖季降水的趋势;在年尺度上,两种数据表现较差。在空间分布上,两种数据类型均能够反映出阿克苏河流域降水自西北向东南递减的空间分布趋势。并且两种数据在平原区的表现均优于山区,低估高海拔地区降水而高估低海拔地区的降水。