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411.
为评价单环刺螠纤溶酶(UFEⅠ)的抗凝血和抗血栓形成作用,并对其免疫原性进行初步研究.文中通过小鼠血液凝固时间实验和大鼠颈动脉血栓形成模型研究UFE Ⅰ抗凝血和抗血栓作用.用纯UFE Ⅰ为抗原免疫小鼠,间接ELISA法测定特异性抗体的动态变化及效价.结果UFE Ⅰ能明显延长小鼠凝血时间,有效抑制大鼠体内的血栓形成,并显著降低大鼠纤维蛋白原含量(FIB),延长活化部分凝血活酶时间(APTT).免疫原性的研究结果为UFE Ⅰ末次免疫小鼠后,产生微量特异性抗体,且抗体水平呈现先升后降的动态变化;方阵法确定了ELISA的优化条件,测定UFE Ⅰ免疫后产生的抗体效价为1∶1 280.本文研究显示,单环刺螠纤溶酶UFEⅠ具有显著的抗凝血和抗血栓形成作用,在小鼠体内产生一过性抗体,但对动物机体不造成负面影响. 相似文献
412.
历史文献中有许多关于渤海"海溢"的记录,但"海溢"是否就是现代意义上的海啸还存在疑问,而且渤海海域基本不存在发生重大海啸的海沟型地震构造背景,因此,渤海地区是否发生过海啸的争论从未停止.本文在分析历史地震和古籍资料的基础上,通过数值模拟分析历史地震引发海啸的可能性,结合对渤海沿岸海啸堆积物的地质调查,认为渤海海域历史上基本没有发生过破坏性海啸事件,即使存在过海啸,到岸浪高也不会高于0.5 m,而且仅限于东营—潍坊一带. 相似文献
413.
Nivaor Rodolfo Rigozo Cláudio Sergio Lisi Mário Tomazello Filho Alan Prestes Daniel Jean Roger Nordemann Mariza Pereira de Souza Echer Ezequiel Echer Heitor Evangelista da Silva Valderez F. Rigozo 《Pure and Applied Geophysics》2012,169(12):2181-2191
This work investigates the behavior of the sunspot number and Southern Oscillation Index (SOI) signal recorded in the tree ring time series for three different locations in Brazil: Humaitá in Amaz?nia State, Porto Ferreira in S?o Paulo State, and Passo Fundo in Rio Grande do Sul State, using wavelet and cross-wavelet analysis techniques. The wavelet spectra of tree ring time series showed signs of 11 and 22?years, possibly related to the solar activity, and periods of 2–8?years, possibly related to El Ni?o events. The cross-wavelet spectra for all tree ring time series from Brazil present a significant response to the 11-year solar cycle in the time interval between 1921 to after 1981. These tree ring time series still have a response to the second harmonic of the solar cycle (5.5?years), but in different time intervals. The cross-wavelet maps also showed that the relationship between the SOI x tree ring time series is more intense, for oscillation in the range of 4–8?years. 相似文献
414.
采用分子生物学方法,以霍乱弧菌lolB为靶基因设计特异性引物,进行了霍乱弧菌的PCR和环介导等温核酸扩增(Loop-mediated isothermal amplification,LAMP)检测技术研究,并对它们的特异性、灵敏性和实际应用进行了比较.结果表明,所建立的PCR检测霍乱弧菌的方法最低检测限为4.0× 103 CFU/ml; LAMP检测方法在65℃下恒温扩增60min,检测限为4.0×101 CFU/ml,反应产物加入荧光染料SYBR Green Ⅰ后反应液呈现明显的绿色;以温和气单胞菌、副溶血弧菌、鳗弧菌及美人鱼弧菌为对照菌株,检测结果均为阴性;霍乱弧菌人工染菌的8种水产品进行PCR及LAMP检测,结果均为阳性,而未染菌组均为阴性;PCR及LAMP检测霍乱弧菌的方法均具有灵敏度较高、特异性强等优点,且LAMP检测霍乱弧菌的方法灵敏度是PCR方法的100倍,更适合于养殖现场检测的推广使用. 相似文献
415.
Sea-ice concentration is a key item in global climate change research.Recent progress in remotely sensed sea-ice concentration product has been stimulated by the use of a new sensor,advanced microwave scanning radiometer for EOS(AMSR-E),which offers a spatial resolution of 6 km×4 km at 89GHz.A new inversion algorithm named LASI(linear ASI) using AMSR-E 89GHz data was proposed and applied in the antarctic sea areas.And then comparisons between the LASI ice concentration products and those retrieved by the other two standard algorithms,ASI(arctic radiation and turbulence interaction study sea-ice algorithm) and bootstrap,were made.Both the spatial and temporal variability patterns of ice concentration differences,LASI minus ASI and LASI minus bootstrap,were investigated.Comparative data suggest a high result consistency,especially between LASI and ASI.On the other hand,in order to estimate the LASI ice concentration errors introduced by the tie-points uncertainties,a sensitivity analysis was carried out.Additionally an LASI algorithm error estimation based on the field measurements was also completed.The errors suggest that the moderate to high ice concentration areas(>70%) are less affected(never exceeding 10%) than those in the low ice concentration.LASI and ASI consume 75 and 112 s respectively when processing the same AMSR-E time series thourghout the year 2010.To conclude,by using the LASI algorithm,not only the seaice concentration can be retrieved with at least an equal quality as that of the two extensively demonstrated operational algorithms,ASI and bootstrap,but also in a more efficient way than ASI. 相似文献
416.
土钉支护结构可靠度分析的电子表格法 总被引:1,自引:0,他引:1
岩土工程中存在大量的不确定性,因此,进行土钉支护结构的可靠度分析是十分必要的。利用Excel的规划求解及VB编程功能,采用电子表格法,建立了适用于分层土体的电子表格计算模型,进行了土钉支护结构内部稳定的可靠度分析,得出了最小可靠指标及其相应的临界滑面位置。建立的电子表格模型由数据输入模块、土钉计算模块、土层计算模块、可靠指标计算模块及优化求解模块5部分组成。模型建立之后,只需改变电子表格中的输入数据,即可适用于不同土钉支护结构的可靠度分析。算例分析表明:方法正确,计算过程简单直观。它既可用于不同工况下土钉支护结构内部稳定的可靠度分析,也可简化为相应情况下的定值法分析。 相似文献
417.
418.
A case study of spatial heterogeneity of soil moisture in the Loess Plateau, western China: A geostatistical approach 总被引:9,自引:2,他引:7
BI Huaxing LI Xiaoyin LIU Xin GUO Mengxia LI Jun Prof. College of Soil Water Conservation Beijing Forestry University Beijing China Key Laboratory of Soil Water Conservation Desertification Combating Ministry of Education China Researcher Ji-xian National Ecological Station of China China Graduate student 《国际泥沙研究》2009,24(1):63-73
Soil moisture distribution shows highly variation both spatially and temporally. This study assesses the spatial heterogeneity of soil moisture on a hill-slope scale in the Loess Plateau in West China by using a geostatistical approach. Soil moisture was measured by time-domain reflectometry (TDR) in 313 samples. Two kinds of sampling scales were used (2 × 2 m and 20 ×20 m) at two soil layers (0-30 cm and 30-60 cm). The general characteristics of soil moisture were analyzed by a classical statistics method, and the spatial heterogeneity of soil moisture was analyzed using a geostatistical approach. The results showed that the spherical model is the best-fit model to simulate soil moisture on the experimental hill-slope. The parameters of this model indicated that the spatial dependence of soil moisture in the selected hill-slope was moderate. Even the 2 × 2 m sampling scale was too coarse to show the detailed spatial variances of soil moisture in this area. The dependent distance increased from 27.4 m to 494.16 m as the sampling scale became coarse (from 2× 2 m to 20 ×20 m). A map of soil moisture was generated by using original soil moisture data and interpolated values determined by the Kriging method. The average soil moisture (area weighted) in the different layers of soil was calculated on the basis of this map (10.94% for the 0-30 cm soil layer, 11.88% for the 30-60 cm soil layer). This average soil moisture is lower than the corresponding average effective soil moisture, which suggests that the soil moisture is not sufficient to support vegetation in this area. 相似文献
419.
420.