Rates of net assimilation and respiration of amino acids by marine bacteria

The selectivity of amino acid assimilation by marine bacteria was examined using seven kinds of¹⁴C-amino acids and the acid hydrolysate of¹⁴C-labelled proteins. It was found that the net assimilation and respiration by marine bacteria followed MICHAELIS-MENTEN kinetics for all of amino acids used in our experiments. Maximum velocities of amino acids were 0.01 to 0.19g carbon/hour per 2×10⁷ cells for net assimilation and less than 0.18g carbon/hour per 2×10⁷ cells for respiration at 20C. The velocity of gross assimilation was found with the following order: phenylalanine>valine, glutamic acid>serine, arginine>tryptophan>glycine. The assimilation velocities of amino acids in these laboratory works showed almost the same order as those in field experiments. The assimilation velocity of an amino acid was influenced by coexisting another amino acids or glucose. The assimilation velocity in lower substrate range of amino acids was directly proportional to the number of bacterial cells in the range from 6×10² to 3×10⁴ cells per ml. No linear relation between the assimilation velocity of amino acids and reciprocal of absolute temperature was found, but a marked bending was observed at 15 to 20C. The velocity at the optimum temperature was three to six times of that at 5C.     

Simultaneous Measurement of Hydrogen Peroxide and Fe Species (Fe(II) and Fe<Subscript>(tot)</Subscript>) in Okinawa Island Seawater: Impacts of Red Soil Pollution

The northern part of Okinawa Island suffers from red soil pollution—runoff of red soil into coastal seawater—which damages coastal ecosystems and scenery. To elucidate the impacts of red soil pollution on the oxidizing power of seawater, hydrogen peroxide (HOOH) and iron species including Fe(II) and total iron (Fe_(tot), defined as the sum of Fe(II) and Fe(III)) were measured simultaneously in seawater from Taira Bay (red-soil-polluted sea) and Sesoko Island (unpolluted sea), off the northern part of Okinawa Island, Japan. We performed simultaneous measurements of HOOH and Fe(II) because the reaction between HOOH and Fe(II) forms hydroxyl radical (•OH), the most potent environmental oxidant. Gas-phase HOOH concentrations were also measured to better understand the sources of HOOH in seawater. Both HOOH and Fe(II) in seawater showed a clear diurnal variation, i.e. higher in the daytime and lower at night, while Fe_(tot) concentrations were relatively constant throughout the sampling period. Fe(II) and Fe_(tot) concentrations were approximately 58% and 19% higher in red-soil-polluted seawater than in unpolluted seawater. Gas-phase HOOH and seawater HOOH concentrations were comparable at both sampling sites, ranging from 1.4 to 5.4 ppbv in air and 30 to 160 nM in seawater. Since Fe(II) concentrations were higher in red-soil-polluted seawater while concentrations of HOOH were similar, •OH would form faster in red-soil-polluted seawater than in unpolluted seawater. Since the major scavenger of •OH, Br⁻, is expected to have similar concentrations at both sites, red-soil-polluted seawater is expected to have higher steady-state •OH concentrations.     

Standing Crops of Planktonic Ciliates and Copepod Nauplii in the Subarctic North Pacific and the Bering Sea in Summer

Abundances and biomasses of planktonic ciliates and copepod nauplii, major components of the microzooplankton community, were investigated in the subarctic North Pacific and the Bering Sea in summer of 1997. Their regional variation was illustrated by demarcating the entire area into five regions. Ciliates always predominated both in abundance (>94%) and biomass (>78%) over nauplii. Regional means of ciliates in the water column were higher in the Alaskan Gyre (120 × 10⁶ cells/m²) and the Western Subarctic Gyre (110 × 10⁶ cells/m²) in terms of abundance, and rich in the Bering Sea Gyre (360 mgC/m²) and the Western Subarctic Gyre (340 mgC/m²) in terms of biomass. By contrast, standing crops of ciliates were poor in the Oyashio Region (67 × 10⁶ cells/m²; 170 mgC/m²) and the Transition Region (64 × 10⁶ cells/m²; 160 mgC/m²). The values of biomass reported here are generally in agreement with the values reported previously from the Bering Sea Gyre and the Alaskan Gyre but are considerably higher than the previous value found in the Western Subarctic Gyre. No significant correlations could be found between chlorophyll a crop and standing crops of ciliates and copepod nauplii over the entire subarctic North Pacific and the Bering Sea during this summer.     

Determination of cadmium,lead and copper in interstitial water by anodic stripping voltammetry

This paper gives the results of determination of cadmium, lead and copper in interstitial water of the coastal sediment, Seto Inland Sea, Japan by using anodic stripping voltammetry. An aliquot of 4–5 ml interstitial water separated from 30–70 ml of sediment is used for the simultaneous determination of ionic cadmium, lead and copper. No significant differences were observed on the content of trace metals between surface water and interstitial water of the top of the sediment.     

Sedimentation rates and heavy metal pollution of sediments in the Seto Inland Sea

In the Hiuchi-Nada area of the Seto Inland Sea, Japan, sedimentation rates were determined with the Pb-210 technique, and heavy metals in sediments were also analyzed. Sediments were collected in twelve short sediment cores and a long sediment core, using 1-m and 6-m gravity corers.The sedimentation rates vary from 0.14 to 0.31 g cm^–2yr^–1. The highest sedimentation rate was observed in the central part of the area, while lower sedimentation rates were observed in the eastern part.In Hiuchi-Nada, a remarkable increase in copper and zinc contents is noticeable as early as the 1800's. Over the past 240 years a copper smelter at the south-western side of this area has been causing serious pollution of the sediment. Now, anthropogenic copper and zinc loads into the sediment are 123 and 376 ton yr^–1 compared to natural copper and zinc loads of 82 and 401 ton yr^–1, respectively. The highest level of copper and zinc pollution was observed in the 1960's, when the relative enrichments above background values (copper; 19 and zinc; 93 mg kg^–1) were 5.5 and 2.8, respectively.     

The coriolis effect against frontogenesis in steady buoyancy-driven circulation

The Coriolis effect on frontogenesis in the transitional region between the inner bay and the outer ocean is studied by running several three-dimensional numerical experiments. The aim is to clarify what external parameters, including the transverse scale of the basin, control the phenomena. The characteristics of the front in each case are described by defining three diagnostic quantities, namely, the sharpness of the front, the intrusion of the front, and the buoyancy difference between the minimum buoyancy region and the surrounding part, as the existence of the minimum buoyancy region is closely related to frontogenesis. The three quantities are shown to depend on two nondimensional numbers,R _of (=f ⁻¹ L ^−2/3 F _b ^1/3 : defined as flux Rossby number) andR _ef (=ν _v ⁻¹ L ^−2/3 F _b ^1-3 H ²: defined as flux Reynolds number).     

Regional distribution of dinoflagellate population off the coasts of Hokkaido in the Japan and Okhotsk seas from spring to Autumn, 1983

The regional distribution of dinoflagellates was investigated in the surface waters surrounding Hokkaido in May, August, and October, 1983. Among the total of 92 species identified, 37 species appeared throughout the investigation period. A similarity analysis identified six assemblages at C_λ=0.66. It was shown that each assemblage was closely related to the currents and the water temperature. Assemblage I consisted of 80% of the total samples and was subdivided into two groups-cold and warm water species in the Tsushima Current. Cold water species were widely spread in the Tsushima and Soya Currents in May and shifted to the Low Saline Water in October while warm water species were dominant in the Tsushima and Soya Currents in October. The regional abundance of shellfish toxic dinoflagellates,Dinophysis fortii andProtogonyaulax tamarensis, was also revealed.     

Interannual fluctuations in recruitment of walleye pollock in the Oyashio region related to environmental changes

The Japanese Pacific walleye pollock (Theragra chalcogramma) stock is the largest stock of this species in Japanese waters. It is a key component of the Oyashio ecosystem. In southern Hokkaido waters, these fish spawn mainly during January and February near the mouth of Funka Bay (FB), and most eggs and larvae are transported into FB. During midsummer juvenile pollock migrate along the southern coast of Hokkaido to a nursery ground on the continental shelf off eastern Hokkaido (Doto area). However, some eggs and larvae are transported southward to the Tohoku region (TR). Transport depends largely on the Oyashio, which generally flows southward along the eastern coasts of Hokkaido and Tohoku. Thus, this stock has two different recruitment routes: FB–Doto and FB–TR. In the 1980s, when the southward flow of the Oyashio was strong, the number of age-2 pollock estimated from a virtual population analysis (VPA) indicated that recruitment to the entire stock remained at a medium level. In the 1990s, when the Oyashio weakened, strong year-classes occurred in 1991, 1994, and 1995, but not in the latter half of the 1990s. Juvenile catches in the TR by commercial fisheries, which can be taken as indices of recruitment level via FB–TR, were high during the 1980s and decreased in the 1990s. Although there was no significant difference in the average number of recruits between the 1980s and the 1990s as estimated from a VPA, the recruitment patterns differed between the two decades. Here, we propose that recruitment routes of this stock shifted in response to environmental changes.     

The study of oceanic turbulent diffusion

Journal of Oceanography - It is indeed a great honor to receive the Oceanographical Society of Japan Prize (1983) for the study of oceanic turbulent diffusion. For this award I owe a great many...     

Coastal Disturbance in Sea Level Propagating along the South Coast of Japan and Its Impact on the Kuroshio

The coastal sea level propagating westward along the south coast of Japan and the impact of the disturbance on the generation of the Kuroshio small meander have been examined. The propagation occurs in sea level variations for periods shorter than 10 days and is remarkable for periods of 4–6 days. Characteristics of the 4–6 day component have been studied using the extended empirical orthogonal function (EEOF). The first and second modes of EEOF are almost in-phase throughout the south coast of Japan. The higher four modes of EEOF are significantly excited when the Kuroshio takes the non-large-meander path, and propagate westward with phase speeds of 2.8 m s⁻¹ (third and fourth modes) and 1.6 m s⁻¹ (fifth and sixth modes) in the Kuroshio region west of Mera in the Boso Peninsula. The analysis shows that more than 70% of the small meanders generate in two months after a significant propagating disturbance reaches south of Kyushu when the velocity of the Kuroshio is high. This effect of coastal disturbance is examined by numerical experiments with a 2.5-layer model in which coastal disturbance is excited by vertical displacement of the upper interface. The result is that offshore displacement of the Kuroshio occurs southeast of Kyushu only in the case of significant upward displacement of the interface under the influence of a high Kuroshio velocity. The significant coastal disturbance, which is associated with upward displacement of the density interface, and a high Kuroshio velocity can therefore be important factors in generating small meanders.