Cell responses to xenobiotics: comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanisms

Multi-drug resistance (MDR) in MCF7 breast cancer cells and multi-xenobiotic resistance (MXR) in mussel (Mytilus edulis) blood cells (MBC) are well known mechanisms that contribute to the decrease in intracellular concentrations of many unrelated but cytotoxic compounds. In the present work, we have carried out comparative investigations of the MDR/MXR protective mechanisms using a rapid colorimetric assay for cell viability and calcein accumulation for MDR/MXR activities. These studies were performed using cultured MCF7 and MBC before and after in vitro exposure to xenobiotics. Our results indicate that a 5-day exposure to doxorubicin or vincristine decreased calcein accumulation in MBC which is consistent with an induction of multi-xenobiotic resistance. The increase in calcein accumulation provoked by 1-h treatment with 50 microM verapamil was much lower in MBC when compared to the P-glycoprotein overexpressing MCF7 cell line. We conclude that such microplate assays could be used in primary cultures of MBC to estimate the effects of various chemicals on MXR activity.     

A multimodel comparison for assessing water temperatures under changing climate conditions via the equilibrium temperature concept: case study of the Middle Loire River,France

This paper investigates three categories of models that are derived from the equilibrium temperature concept to estimate water temperatures in the Loire River in France and the sensitivity to changes in hydrology and climate. We test the models' individual performances for simulating water temperatures and assess the variability of the thermal responses under the extreme changing climate scenarios that are projected for 2081–2100. We attempt to identify the most reliable models for studying the impact of climate change on river temperature (T_w). Six models are based on a linear relationship between air temperatures (T_a) and equilibrium temperatures (T_e), six depend on a logistic relationship, and six rely on the closure of heat budgets. For each category, three approaches that account for the river's thermal exchange coefficient are tested. In addition to air temperatures, an index of day length is incorporated to compute equilibrium temperatures. Each model is analysed in terms of its ability to simulate the seasonal patterns of river temperatures and heat peaks. We found that including the day length as a covariate in regression‐based approaches improves the performance in comparison with classical approaches that use only T_a. Moreover, the regression‐based models that rely on the logistic relationship between T_e and T_a exhibit root mean square errors comparable (0.90 °C) with those obtained with a classical five‐term heat budget model (0.82 °C), despite a small number of required forcing variables. In contrast, the regressive models that are based on a linear relationship T_e = f(T_a) fail to simulate the heat peaks and are not advisable for climate change studies. The regression‐based approaches that are based on a logistic relationship and the heat balance approaches generate notably similar responses to the projected climate changes scenarios. This similarity suggests that sophisticated thermal models are not preferable to cruder ones, which are less time‐consuming and require fewer input data. Copyright © 2012 John Wiley & Sons, Ltd.     

Multi-method investigation of mass transfer mechanisms in a retrogressive clayey landslide (Harmalière,French Alps)

Landslides - The mass transfer mechanisms in landslides are complex to monitor because of their suddenness and spatial coverage. The active clayey Harmalière landslide, located 30 km south of...     

The IAU 2009 system of astronomical constants: the report of the IAU working group on numerical standards for Fundamental Astronomy

In the 2006?C2009 triennium, the International Astronomical Union (IAU) Working Group on Numerical Standards for Fundamental Astronomy determined a list of Current Best Estimates (CBEs). The IAU 2009 Resolution B2 adopted these CBEs as the IAU (2009) System of Astronomical Constants. Additional work continues to define the process of updating the CBEs and creating a standard electronic document.     

Determination of Boron Concentration in Geochemical Reference Materials Extracted by Pyrohydrolysis and Measured by ICP‐MS

This article presents new boron concentrations for nine geochemical reference materials (GS‐N, FK‐N, GL‐O, BX‐N, DT‐N, AN‐G, GH, Mica‐Fe, Mica‐Mg). After extraction by a modified pyrohydrolysis technique, boron concentrations were measured by ICP‐MS. The blank levels for the whole procedure were 0.091 ± 0.020 ng ml^?1 or 14 ± 5 ng of boron in total. The method was first validated by measuring nine reference materials with known boron concentrations. The determined boron concentrations are all within the range of recommended or published values, which means that the yields were 100%, and show precisions below 10% for samples containing over 2 μg g^?1 of boron.     

Deformed Messinian markers in the Cyprus Arc: tectonic and/or Messinian Salinity Crisis indicators?

This paper focuses on Messinian Salinity Crisis (MSC) evaporites in the Cyprus Arc (eastern Mediterranean) using high‐resolution reflection seismic and multi‐beam data. The results shed new light on the Miocene to Present tectonic evolution of this area and contribute to our general knowledge of the MSC in a deep basin setting. The evaporites and overlying formations show a complex deformation pattern due to a combination of thick‐skinned plate‐tectonic convergence and thin‐skinned disharmonic deformation related to the mobile evaporite‐bearing unit. Several MSC markers are identified and precisely mapped: the base of the MSC unit is a ‘decollement’ level, whereas the top is clearly identified as a toplap surface. Intra‐MSC markers and two MSC subunits are identified and mapped over the entire study area. The geometry of MSC markers shows that the lower MSC subunit was deposited in a relatively quiet tectonic setting. The nature of the anisopachous upper unit indicates a syn‐depositional phase of large‐scale plate‐tectonic activity. A thin‐skinned phase of compressional deformation during the Late Miocene affected the entire MSC unit, overlain by undeformed Pliocene–Quaternary layers. A second thin‐skinned phase, well expressed in the bathymetry, occurred from the Pliocene to Recent, resulting in extensional gravity‐gliding within the evaporites and the Pliocene–Quaternary sequence. We show that the MSC had a dramatic impact on the regional structure. For instance, the erosive nature of the top of the MSC unit is linked to the desiccation episode rather than to the cessation of tectonic activity. This particularly strong and short‐lived erosion may have been enhanced by the regional effects of the MSC, owing to differential uplift/subsidence caused by the drawdown. The evaporites are essential markers for constraining the tectonic framework, provided that active deformation can be distinguished from passive gliding associated with extensional/contractional deformation.     

Mapping and spatial analysis of the soybean agricultural frontier in Mato Grosso, Brazil, using remote sensing data

The main pioneer frontier considered by geographers is the Amazonian pioneer frontier. The occupation of the Brazilian territory has been carried out through successive economic cycles. Currently, the expansion of soybean crops in Amazonia is considered as the last economic cycle involving new migrations to still unexplored areas. Mapping this frontier is necessary in order to better understand its drivers and think about efficient land use policies to struggle its progress. In this paper, we propose an innovative methodology for mapping the agricultural frontier in the Amazonian state of Mato Grosso (Brazil) using satellite data acquired during the 2000–2006 period. We assume that the frontier evolves through successive land-use stages such as wildlands, non-croplands, extensive croplands and intensive croplands areas. The transitions between two stages can then be monitored through land-use change analysis. A particular emphasis is given on three land-use transitions named “deforestation”, “economic” and “intensification sub-frontiers”. In order to adapt this model for the use of remote sensing data, we defined (1) a relevant spatial scale corresponding to an agrarian locality, (2) relevant indices to qualify the land-use types and (3) rules applied on land-use types to define the frontier’s location. Our results highlight the dynamic of the agricultural frontier towards northern areas. We identified four main agricultural regions with different levels of agricultural intensification. We finally discuss these results with respect to governmental policies and economics for a better understanding of the frontier’s dynamic.     

Soil CO2 flux baseline in an urban monogenetic volcanic field: the Auckland Volcanic Field, New Zealand

The Auckland Volcanic Field (AVF) is a dormant monogenetic basaltic field located in Auckland, New Zealand. Though soil gas CO₂ fluxes are routinely used to monitor volcanic regions, there have been no published studies of soil CO₂ flux or soil gas CO₂ concentrations in the AVF to date or many other monogenetic fields worldwide. We measured soil gas CO₂ fluxes and soil gas CO₂ concentrations in 2010 and 2012 in varying settings, seasons, and times of day to establish a baseline soil CO₂ flux and to determine the major sources of and controlling influences on Auckland's soil CO₂ flux. Soil CO₂ flux measurements varied from 0 to 203 g m^?2 day^?1, with an average of 27.1 g m^?2 day^?1. Higher fluxes were attributed to varying land use properties (e.g., landfill). Using a graphical statistical approach, two populations of CO₂ fluxes were identified. Isotope analyses of δ¹³CO₂ confirmed that the source of CO₂ in the AVF is biogenic with no volcanic component. These data may be used to assist with eruption forecasting in the event of precursory activity in the AVF, and highlight the importance of knowing land use history when assessing soil gas CO₂ fluxes in urban environments.     

An archaeo-seismological study of the Nîmes Roman aqueduct,France: indirect evidence for an <Emphasis Type="Italic">M</Emphasis> > 6 seismic event?

This paper presents a synthesis of a multidisciplinary study carried out along the Nîmes Roman aqueduct, located in the southeast part of France. The study was motivated by archaeologists attempting to explain the partial destructions of only one aerial bridge of the aqueduct (Pont de la Lône). Given its close proximity to the Nîmes seismically active fault, a possible seismic origin for the destructions was invoked. Seismologists and structural engineers thus carried out a variety of field and numerical investigations to test the seismic hypothesis. Supporting field evidence was found first along the aerial bridge section of the aqueduct: broken stalactites, arch warping, cracks and destruction of the bridge just above a breast wall shortly after its construction. Secondly, the underground part of the canal was analysed: irregularity in the thickness of calcite deposits of the canal walls, presence of numerous cracks, horizontal shift of the otherwise linear structure of the canal and presence of calcite twins in the deposits, found only where the canal crosses the Nîmes fault system. Numerical modelling and experimental results show that (1) only the Pont de la Lône would have suffered serious damage under seismic solicitation (assuming an M6 earthquake at 10 km distance). The second aerial bridge, the three levels arches Pont du Gard, an historical monument still standing today, would have suffered less damage, due to its very different fundamental frequency of around 0.4 Hz, far from the amplified seismic near-field spectrum; (2) the numerical models also show that a fall of the canal wall would require a higher magnitude event (M > 6); (3) the presence of calcite twinning requires a differential static stress of 4 MPa or greater, which can only be achieved very close to the fault rupture of a M > 6 earthquake; (4) finally, local surface rupturing of such a fault would also corroborate the hypothesis that the observed offset of the canal may be partly seismically induced. Although other possible origins for each individual evidence may not be excluded, the observed spatiotemporal concentration of architectural/geological anomalies together with the numerical results allow us to support a possible co-seismic origin for these disorders, indirectly attesting to the potential seismic activity (M > 6) of the nearby Nîmes fault. Furthermore, following the conclusions of archaeological studies, the disorders occurred between 250 and 350 year AD, thus providing timing for this possible seismic event, an essential parameter in seismic hazard analysis. These results validate the archaeo-seismological approach as a tool that may help improve the knowledge of major infrequent earthquakes in areas of moderate seismic activity.