全文获取类型
收费全文 | 3129篇 |
免费 | 311篇 |
国内免费 | 739篇 |
专业分类
测绘学 | 208篇 |
大气科学 | 302篇 |
地球物理 | 1232篇 |
地质学 | 1475篇 |
海洋学 | 408篇 |
天文学 | 127篇 |
综合类 | 138篇 |
自然地理 | 289篇 |
出版年
2024年 | 19篇 |
2023年 | 46篇 |
2022年 | 58篇 |
2021年 | 57篇 |
2020年 | 39篇 |
2019年 | 44篇 |
2018年 | 62篇 |
2017年 | 25篇 |
2016年 | 52篇 |
2015年 | 47篇 |
2014年 | 92篇 |
2013年 | 70篇 |
2012年 | 128篇 |
2011年 | 184篇 |
2010年 | 76篇 |
2009年 | 92篇 |
2008年 | 190篇 |
2007年 | 160篇 |
2006年 | 196篇 |
2005年 | 187篇 |
2004年 | 150篇 |
2003年 | 134篇 |
2002年 | 130篇 |
2001年 | 90篇 |
2000年 | 139篇 |
1999年 | 80篇 |
1998年 | 85篇 |
1997年 | 72篇 |
1996年 | 86篇 |
1995年 | 96篇 |
1994年 | 92篇 |
1993年 | 133篇 |
1992年 | 83篇 |
1991年 | 138篇 |
1990年 | 85篇 |
1989年 | 100篇 |
1988年 | 86篇 |
1987年 | 80篇 |
1986年 | 41篇 |
1985年 | 67篇 |
1984年 | 52篇 |
1983年 | 53篇 |
1982年 | 33篇 |
1981年 | 29篇 |
1980年 | 42篇 |
1965年 | 11篇 |
1964年 | 15篇 |
1959年 | 16篇 |
1958年 | 21篇 |
1957年 | 14篇 |
排序方式: 共有4179条查询结果,搜索用时 125 毫秒
961.
Performance comparison of two biotic indices measuring the ecological status of water bodies in the Southern Baltic and Gulf of Lions 总被引:1,自引:1,他引:0
Fleischer D Grémare A Labrune C Rumohr H Berghe EV Zettler ML 《Marine pollution bulletin》2007,54(10):1598-1606
Two biotic indices, ATZI Marine Biotic Index (AMBI) and Benthic Quality Index (BQI) have been recently introduced within the EC Water Framework Directive to assess the quality of marine habitats: both are based on sensitivity/tolerance classification and quantitative information on the composition of soft-bottom macrofauna. Their performance, especially with regard to sampling effort was assessed based on two data sets collected in Southern Baltic and one from the Gulf of Lions Mediterranean. AMBI was not affected by sampling effort but BQI was. Two modifications were proposed for BQI (i.e., BQI) (1) the removal of the scaling term (i.e., BQI(W)), and (2) the replacement of the scaling term by different scaling term (i.e., BQI(ES)). Both modified BQIs were largely independent of sampling effort. Variability was slightly lower for BQI(W) than for BQI(ES). BQI was highly correlated with BQI(W) and with BQI(ES) both in the Southern Baltic and in the Gulf of Lions. However, the proportions of stations, which were not attributed the same ecological quality status (EcoQ) when using BQI and its two modified forms were always high. Differences in ecological classification were mostly due to the scales used to infer EcoQ. Based on this study we recommend to use BQI(ES) in future studies because it apparently constitutes the best compromise in (1) being independent of sampling effort, (2) limiting the variability in computation in relation with sampling effort, (3) being correlated with BQI and corresponding EcoQ. 相似文献
962.
Antarctic marine bacteria were screened for their ability to degrade polychlorinated biphenyls (PCB) as the sole carbon and energy source at both 4 degrees C and 15 degrees C. PCB-degrading isolates (7.1%) were identified by sequencing their 16S rDNA as Pseudoalteromonas, Psychrobacter and Arthrobacter members. One representative isolate per genera was selected for evaluating the biodegradative potential under laboratory scale and phenotypically characterized. Removal of individual PCB congeners was between 35.6% and 79.8% at 4 degrees C and between 0.4% and 82.8% at 15 degrees C. Differences in the removal patterns of PCB congeners were observed in relation to the phylogenetic affiliation: Arthrobacter isolate showed similar biodegradation efficiencies when growing at 4 degrees C and 15 degrees C, while Pseudoalteromonas better degraded PCBs at 15 degrees C. No biodegradation was detected for Psychrobacter isolate at 4 degrees C. Results obtained highlight the occurrence of PCB-degrading bacteria in Antarctic seawater and suggest the potential exploitation of autochthonous bacteria for PCB bioremediation in cold marine environments. 相似文献
963.
Kurihara R Watanabe E Ueda Y Kakuno A Fujii K Shiraishi F Hashimoto S 《Marine pollution bulletin》2007,54(9):1315-1320
Estrogenic activity was determined in sediments collected from Tokyo Bay. Sampling was performed at five stations including the site near the sewage treatment plant. The most estrogenic sediment collected near the sewage treatment plant was fractionated into ten fractions using normal-phase high-performance liquid chromatography. Chemical analysis was carried out for each fraction and nonylphenol (NP, 20,700 ng g−1 dry wt) was detected at a higher concentration than estron (2.39 ng g−1 dry wt) and 17β-estradiol (<0.7 ng g−1 dry wt). Furthermore, each fraction was administered to male mummichogs (Fundulus heteroclitus), and vitellogenin (Vtg) was measured after two weeks. The induction of Vtg was observed; this estrogenic potency could be attributed to the NP content in this fraction. This is the first report to suggest that the high NP concentration in the sediments from Tokyo Bay has the potential to induce Vtg in wild fish. 相似文献
964.
The most important objective within the European Water Framework Directive (WFD) is to achieve a ‘good ecological status’ (GES) for all waters, by 2015. Some methodologies have been developed for assessing GES within natural water bodies, in which the ecological status is a perceived or measured deviation from a reference condition. However, the WFD also consider ‘Heavily modified water bodies’ (HMWB) (a water body resulted from physical alterations by human activity, which substantially change its hydrogeomorphological character, e.g. a harbour). In implementing the WFD, environmental managers are required to assess the status of HMWBs in terms of achieving ‘Good Ecological Potential’ (GEP). This contribution defines and studies GEP from an ecological point of view, taking into account some ecological restoration principles. Finally, this contribution gives some guidance on how establish GEP, using as example a harbour within the North East Atlantic. 相似文献
965.
Vlahogianni T Dassenakis M Scoullos MJ Valavanidis A 《Marine pollution bulletin》2007,54(9):1361-1371
Mussels are used as sentinel organisms and bioindicators to evaluate the toxic effects of chemical pollutants in marine organisms, especially heavy metals, representing an important tool for biomonitoring environmental pollution in coastal areas. Antioxidant defence enzymes play an important role in cellular antioxidant defence systems and protect from oxidative damage by reactive oxygen species (ROS). Indigenous mussels Mytilus galloprovincialis of the Saronikos Gulf of Greece were used for monitoring heavy metal pollution in three polluted sites in the area and in one unpolluted site. Seasonal variations of the activity of antioxidant defence enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as lipid peroxidation (LP) were measured as biomarkers in a period of three years in relation to concentrations of trace metals in their gills and mantle and compared to mussels from an unpolluted sampling site. SOD activity increased at least 2 fold at the polluted sites when compared to the control site (the high activity was recorded in the spring time). CAT activity was increased 2-3 times at the polluted sites, with high activity in the winter and spring time, compared to the control site. LP concentration was twice higher at the polluted sites, following the same seasonal pattern. Trace metals contents in mussels collected at polluted sites were 3-4 fold higher compared to the control site and showed moderate variations along the months, with a winter maximum followed by a summer pre-spawning minimum matching the seasonal trends of temperature and salinity. Our results showed that metal pollution in the Elefsis Bay (the most polluted coastal area) causes relatively medium levels of oxidative stress in tissues of mussels due to cellular oxy-radical generation. This study, which is the first in the area, showed that seasonal variations of the activity of antioxidant defence enzymes and LP concentrations in mussels can be used as potential biomarkers of toxicity for long-term monitoring in marine coastal ecosystems. 相似文献
966.
Covelli S Piani R Acquavita A Predonzani S Faganeli J 《Marine pollution bulletin》2007,55(10-12):436-450
The role of suspended particulate matter (SPM) as an important carrier of mercury (Hg) dispersed into the Gulf of Trieste and in the adjacent Grado lagoon (Northern Adriatic Sea) was studied during a high Isonzo River inflow and the resulting river plume formation. Despite the fact that extreme flood events are rare during the year, they account for most of the PHg influx (37-112 ngL(-1)) into the Gulf of Trieste. When the river plume is diverted to the SW under the influence of an E-NE wind, the tidal flux acts as a "transport belt" carrying the PHg, mostly inorganic, into the Grado lagoon. A preliminary estimation indicates that the amount of PHg entrapped in the lagoon basin following a tidal semi-cycle accounts for 1.4 kg/12h, which corresponds to about 49% of the total Hg carried by the tidal flow. These findings should be considered in future remediation strategies in the lagoon environment. 相似文献
967.
968.
Licciano M Terlizzi A Giangrande A Cavallo RA Stabili L 《Marine environmental research》2007,64(4):504-513
The present study investigates the effect of the filtering activity of Sabella spallanzanii on the culturable heterotrophic bacterial community through the comparison of the bacterial diversity in transplanted polychaetes and the surrounding seawater. For isolation of culturable heterotrophic bacteria, seawater samples as well as polychaete homogenates were plated in triplicates onto Bacto Marine Agar 2216 (Difco). All the colonies grown were isolated, subcultured and identified by several morphological, biochemical and cultural methods. Some bacterial genera showed higher average abundances in polychaetes than in seawater (i.e. Lucibacterium and Photobacterium). Aeromonas represented a conspicuous component of the bacterial community both in S. spallanzanii and seawater. The presence of Cytophaga and Pseudomonas was also relevant in the examined seawater samples. The selective concentrations of some bacterial genera inside S. spallanzanii either by grazing on bacteria or their capability as bacterial reservoirs, provides evidence for the role of macrobenthic invertebrates as key determinants for microbial diversity. 相似文献
969.
Monari M Cattani O Serrazanetti GP Selli A Pagliuca G Zironi E O'Hara SC Livingstone DR 《Marine environmental research》2007,63(3):200-218
The effects of water-borne exposure to benzo[a]pyrene (36 h; celite-bound 0.44 mg L(-1) B[a]P) on cytochrome P450 (CYP) and superoxide dismutases (SODs) were examined in digestive gland of the blood clam, Scapharca inaequivalvis. B[a]P accumulation and elimination were rapid, with maximum whole-body concentrations of 1.78 ng g(-1) wet wt after 12 h of treatment, followed by a progressive decline to 0.89 ng g(-1) at 36 h. The presence of B[a]P resulted in an increase in total CYP of digestive gland microsomes from 54+/-14 to 108+/-21 pmol/mg protein (mean+/-SD; p<0.05, 24 h). Increases were also seen in microsomal CYP1A1/1A2-immunopositive protein (50.5 kDa app. mol. wt; p<0.05), but not CYP2E1-immunopositive protein (49 kDa app. mol. wt.), indicating a specific response of the former isoform. Exposure to B[a]P produced a steady increase in Mn-SOD digestive gland activity (p<0.01; p<0.05) but no significant change in Cu/Zn-SOD activity. The respective proteins, measured by western blotting, were not significant induced after B[a]P exposure. Cu/Zn-SOD and Mn-SOD activities were correlated with total CYP levels (r=0.96 and 0.63, respectively), indicating a role for CYP in reactive oxygen species (ROS) production during exposure. Both 'NADPH-independent' and NADPH-dependent metabolism of B[a]P by digestive gland microsomes was seen, producing mainly 1,6-, 3,6- and 6,12-diones, with some phenols and 7,8-dihydrodiol; putative protein adducts were also formed. Redox cycling of the diones may also have contributed to ROS production, leading to the increased SOD activities. 相似文献
970.
de Abreu Corrêa A Albarnaz JD Moresco V Poli CR Teixeira AL Oliveira Simões CM Monte Barardi CR 《Marine environmental research》2007,63(5):479-489
The State of Santa Catarina produces the greatest quantity of edible mollusks in Brazil. To guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. A self-purification or "depuration" system that eliminates Salmonella enterica serovar Typhimurium contamination from oysters has been developed and evaluated. The depuration process occurred within a closed system, in which 1000 L of water was recirculated for 24 h. The water was sterilized with ultraviolet (UV) light, chlorine, or both together. Oysters (Crassostrea gigas) artificially contaminated with S. typhimurium were harvested every 6 h. Samples of oyster tissue were excised and both the presence and numbers of bacteria were determined. Combined UV light and chlorine treatments resulted in total elimination of bacteria within 12 h. Polymerase chain reaction detected bacteria in water exposed to the three treatments. This pioneering study is the first of its kind in Brazil and represents a major contribution to commercial mollusk culture in this country. 相似文献