一秋龄性成熟中华绒螫蟹的生物学──1.外部形态特征及性腺变化

极大螺旋藻藻体ＳＯＤ聚丙烯酰胺梯度凝胶电泳呈现４条同工酶带,Ｈ２Ｏ２明显抑制该酶活性,ＫＣＮ对酶活性无影响,确认为Ｆｅ－ＳＯＤ。经硫酸铵分部盐析,离子交换柱层析及凝胶过滤,纯化到电泳单斑点均一程度。纯化的Ｆｅ－ＳＯＤ分子量为３９．３ＫＤ,亚基分子量为２０ＫＤ,金属元素分析表明,每个亚基含０．５５个Ｆｅ原子。该酶在紫外区最大吸收峰值为２７５．８ｎｍ,该酶氨基酸组成与蓝,绿藻和高等植物的Ｆｅ－ＳＯＤ相     

西藏尼洋河水环境特征多元统计分析

自2008年11月到2009年10月按照月份监测西藏林芝地区尼洋河水环境特征.结果显示,根据Pearson相关性矩阵,可将尼洋河26项理化指标分为3大类,分别是低度相关性理化指标、中度相关性理化指标和高度相关性理化因子.析因分析显示,可将26项理化指标分为4大类,第1类为尼洋河水体常规的理化指标、第2类为尼洋河水体硬度相关指标、第3类为海拔及第4类为影响水生生物生长的水质理化指标.PCA分析发现,平水期、丰水期以及枯水期水环境特征明显不同,以上3期各自聚为一类,而尼洋河沿程方面,水环境特征则不存在差异.构建了尼洋河海拔、底层水温与相关理化因子的一元回归方程.建议加强对尼洋河水环境的监测工作,积极推进尼洋河水域生态环境的可持续发展进程.     

Status of two <Emphasis Type="Italic">Coreius</Emphasis> species in the Three Gorges Reservoir,China

Dam construction alters natural flow regimes which, in turn, cause significant changes in fish communities during and after impoundment. The construction of the Three Gorges Reservoir, from impoundment of the Changjiang (Yangtze) River, China, may have affected native fish species. Thus, the status of two lotic freshwater fish species, Coreius heterodon and C. guichenoti, were monitored in the Three Gorges Reservoir, including fish abundance, individual composition, growth, condition, and mortality. Data on both species were gathered from upstream, midstream and downstream areas of the reservoir and, where available, from studies published before and after dam construction. Lower abundance, slower growth, a less diversified age structure, poorer fish condition (indicated by hepatosomatic index) and higher mortalities were recorded in sites nearest the dam compared with upstream areas. Furthermore, after final impoundment, individual Coreius species inhabiting the area changed, with young individuals becoming more abundant, while upstream of the reservoir the two Coreius species became smaller at a given age. The results show that the status of the two Coreius species was subject to dramatic changes after impoundment.     

Spatial distribution and abundance of small fishes in Xiaosihai Lake, a shallow lake along the Changjiang (Yangtze) River, China

Spatial distribution and abundance of small fishes were studied in autumn 2007 in the Xiaosihai Lake, a shallow lake along the middle reach of the Changjiang (Yangtze) River. Based on the plant cover, the lake was divided into three major habitats: Myriophyllum spicatum habitat (MS habitat), Trapa bispinosa habitat (TB habitat), and non-vegetation habitat (NV habitat). A modified pop-net was used for quantitative sampling of small fishes in the three habitats, and the Zippin’s removal method was used for estimating densities of the small fishes. A total of 13 species belonging to 5 families were collected, with 11 species in MS habitat, 7 species in TB habitat, and 5 species in NV habitat. Habitat type had significant effect on the spatial distribution of small fishes. The Shannon-Wiener diversity index in the MS, TB and NV habitats were 1.28, 0.56 and 0.54, respectively. The total density and biomass of small fishes were significantly higher in the MS habitat (13.68 ind/m² and 4.44 g/m²) than in the TB habitat (1.41 ind/m² and 0.54 g/m²) and the NV habitat (1.08 ind/m² and 0.40 g/m²), and were not significantly different between the TB habitat and the NV habitat. Water depth had no significant effect on spatial distribution of the small fishes. It was suggested that vegetation type played an important role in habitat selectivity of small fishes, and the presence of submersed vegetation should be of significance in the conservation of small fish diversity.     

鄱阳湖鱼类资源及渔业利用

1997年冬至2000年春,对我国最大淡水湖鄱阳湖的鱼类与渔业利用状况进行了调查.在1955年至2000年期间,鄱阳湖累计记录鱼类136种,隶属于25科78属;其中鲤科鱼类最多,有71种,占总种数的52.2%,其次是鲿科,12种,占8.8%本次调查期间共记录鱼类101种,新记录鱼类6种,即亮银鮈、洞庭小鳔鮈、光唇蛇鮈、短须鱊、方氏鳑鲏和粘皮鲻鰕虎鱼.还分析了该湖鱼类区系组成的变化、主要渔具的渔获物组成特征以及渔产量动态,提出了未来湖泊渔业可持续发展的建议.     

云南高原渔洞水库鱼类空间分布格局及主要影响因子

鱼类控藻是常用的生物控藻技术之一,其前提需明确水体中鱼类群落结构及分布特征.为科学调整渔洞水库鱼类群落结构,制定合理的生物操纵策略,2013年7月运用Simrad EY60型分裂波束回声探测仪对该水库进行了水声学探测,在结合多网目复合刺网（网目=8.5、4.0、12.5、2.0、11.0、1.6、2.5、4.8、3.1、1.0、7.5和6.0 cm）取样的基础上,研究了水库鱼类群落结构及其空间分布特征,并初步探讨了影响渔洞水库鱼类空间分布的关键因素.调查中在渔洞水库共采集到鱼类5科12种,其平均密度为638.4±310.2 ind./1000 m³.鱼类在水库中呈不均匀分布,库首、库中、库湾及库尾鱼类密度分别为521.5±371.3、561.9±189.2、653.7±323.7和1137.1±90.4 ind./1000 m³,呈逐渐增大趋势,且库尾鱼类的平均个体要大于库首、库中和库湾;在垂直方向上,97.6%的鱼类都分布在水面以下0~10 m的水层,深度超过10 m的水层,鱼类所占比例只有2.4%;0~20 m水层鱼类的平均目标强度最大（-58.6 dB）,20~40 m水层鱼类平均目标强度最小（-63.9 dB）.MRT预测模型表明影响鱼类空间分布的关键环境因子为水温和水深.基于渔洞水库的鱼类组成和空间分布特征,建议一方面加大鲢、鳙放养量,另一方面从所属水系引进中上层土著肉食性鱼类来调整鱼类群落结构,控制藻类生物量.     

一秋龄性成熟中华绒螫蟹的生物学──1.外部形态特征及性腺变化

本文描述了一秋龄性腺成熟中华绒螯蟹（简称河蟹）的外部特征并与一秋龄未成熟及二秋龄成熟河蟹进行比较,同时给出了体重与壳宽的回归方程．保安湖的研究结果表明,对于一秋龄雌蟹,体重大于２９．８ｇ（壳宽为４．０３ｃｍ）全部成熟,而体重小于１３．１ｇ（壳宽为３．００ｃｍ）均不成熟,体重在１３、１－２９．８ｇ之间,既有成熟又有未成熟．对于一秋龄雄蟹,体重大于２６．０ｇ（壳宽３．９１ｃｍ）,全部成熟,而于１２．０ｇ壳宽２．６６ｃｍ）均未成熟,体重在１２．０－２６．０ｇ之间,既有成熟者又有未成熟个体．对一秋龄成熟河蟹成熟系数逐月变化的观察表明,在翌年３月份达到峰值,雌蟹为１２． ３２± １． ７５％,雄蟹为 ４． ２４± ０． ０６％;此后,性腺迅速退化,至翌年七月上旬,卵巢退变成细索状结构．己无完整的卵粒．     

Status and historical changes in the fish community in Erhai Lake

Erhai Lake is the second largest freshwater lake on the Yunnan Plateau, Southwest China. In recent decades, a number of exotic fish species have been introduced into the lake and the fish community has changed considerably. We evaluated the status of the fish community based on surveys with multimesh gillnet, trap net, and benthic fyke-net between May 2009 and April 2012. In addition, we evaluated the change in the community using historical data (1952–2010) describing the fish community and fishery harvest. The current fish community is dominated by small-sized fishes, including Pseudorasbora parva, Rhinogobius giurinus, Micropercops swinhonis, Hemiculter leucisculus, and Rhinogobius cliffordpopei. These accounted for 87.7% of the 22 546 total specimens collected. Omnivorous and carnivorous species dominated the community. A canonical correspondence analysis (CCA) plot revealed that the distribution of fishes in the lake is influenced by aquatic plants, water temperature, pH, and season. The abundance of indigenous species has declined sharply, and a majority of endemic species have been extirpated from the lake (a decrease from seven to two species). In contrast, the number of exotic species has increased since the 1960s to a total of 22 at present. The fishery harvest decreased initially following the 1960s, but has since increased due to the introduction of non-native fish and stocking of native fish. The fishery harvest was significantly correlated with total nitrogen, not total phosphorus, during the past 20 years. Based on our results, we discuss recommendations for the restoration and conservation of the fish resources in Erhai Lake.     

西藏尼洋河水生生物群落时空动态及与环境因子关系:2.着生藻类

本文于2008-2009年按照季节调查了西藏地区尼洋河着生藻类群落的组成、丰度和多样性,并运用多元统计方法定量分析了着生藻类的空间和季节变化特征及其与主要环境因子之间的关系.结果显示,尼洋河着生藻类共计6门30科49属,其中硅藻为优势着生藻类.着生藻类的Shannon-Wiener多样性指数在尼洋河中游最高,在中上游河段和中下游河段呈下降趋势,符合中间高度膨胀(mid-altitude bulge)假说.着生藻类的总丰度、物种丰富度以及Shannon-Wiener多样性指数在尼洋河沿程不存在显著差异,总丰度在各个季节之间不存在显著差异,但物种丰富度在秋季和冬季存在显著差异,夏、秋季着生藻类的Shannon-Wiener多样性指数显著高于冬、春季.主成分分析(PCA)表明,随着海拔的升高,着生藻类的物种丰富度和总丰度呈递减趋势;典范对应分析(CCA)表明,尼洋河部分硅藻与理化因子相关联,如:双壁藻(Diploneis)的丰度与总磷相关联,窗纹藻(Epithemia)的丰度与铵态氮相关联,双菱藻属(Surirella)的丰度与pH值相关联;部分绿藻与理化因子相关联,如:小球藻属(Chlorella)、栅藻属(Scenedesmus)、溪菜属(Prasiola)的丰度与水温相关联,小椿藻(Characium)的丰度与硬度相关联,转板藻(Mougeotia)的丰度与表层溶氧、矿化度相关联,新月藻属(Closterium)的丰度与碱度相关联;分类回归树(CART)模型预测了尼洋河着生藻类时空分布与主要环境因子之间的定量关系,即低海拔水域的着生藻类总丰度较高海拔高,高矿化度水域的着生藻类总丰度较低矿化度的高,不能用影响尼洋河着生藻类种类和数量的环境因子来解释着生藻类的Shannon-Wiener多样性指数.这些关键环境因子对尼洋河水域着生藻类的时空变化有着重要的指示作用,建议加强对着生藻类及这些环境因子的关注,保障尼洋河水域生态环境的可持续发展.     

Spatial distribution and abundance of small fishes in Xiaosihai Lake, a shallow lake along the Changjiang(Yangtze) River, China

Spatial distribution and abundance of small fishes were studied in autumn 2007 in the Xiaosihai Lake, a shallow lake along the middle reach of the Changjiang (Yangtze) River. Based on the plant cover, the lake was divided into three major habitats: Myriophyllum spicatum habitat (MS habitat), Trapa bispinosa habitat (TB habitat), and non-vegetation habitat (NV habitat). A modified pop-net was used for quantitative sampling of small fishes in the three habitats, and the Zippin’s removal method was used for estimating densities of the small fishes. A total of 13 species belonging to 5 families were collected, with 11 species in MS habitat, 7 species in TB habitat, and 5 species in NV habitat. Habitat type had significant effect on the spatial distribution of small fishes. The Shannon-Wiener diversity index in the MS, TB and NV habitats were 1.28, 0.56 and 0.54, respectively. The total density and biomass of small fishes were significantly higher in the MS habitat (13.68 ind/m2 and 4.44 g/m2) than in the TB habitat (1.41 ind/m2 and 0.54 g/m2) and the NV habitat (1.08 ind/m2 and 0.40 g/m2), and were not significantly different between the TB habitat and the NV habitat. Water depth had no significant effect on spatial distribution of the small fishes. It was suggested that vegetation type played an important role in habitat selectivity of small fishes, and the presence of submersed vegetation should be of significance in the conservation of small fish diversity.