Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

Measurement of the signal to noise ratio in seismic profiling

The measurement of the signal to noise ratio in continuous seismic profiling systems is carried out by means of a small general purpose digital computer equipped with an analog to digital converter. The computer is programmed to compute and compare the power spectrum of a sample of noise to the power spectrum of a sample of noise plus an echo. Power spectra of some twenty to fifty shots are averaged to yield reliable estimates of the signal to noise ratio as a function of frequency. The observed spectra agree well with spectra calculated from signals measured near to an air-gun. This measurement of signal to noise ratio as a function of frequency provides unambiguous information for the adjustment of the necessary band-pass filter thus improving the operation of a continuous seismic profiler.     

Effect of Sea Level Variation on Tidal Characteristic Values for the East China Sea

1 .IntroductionTheglobalairtemperatureroseabout 0 .5～ 0 .6°Coverthepast 2 0thcentury ,andtheglobalmeansealevelincreasedbyabout2 0cmduringtheperiod .Theregionalmeansealevelriseswiththerisingglobalmeansealevel.Zuoetal.( 1 997)indicatedthatthemeanrisingrateofabsolutemeansealevelalongtheChinacoastontheassumptionofunifiedisostaticdatumis 2mm a .Woodworth( 1 999)analyzedsealevelspanning 1 76 8tothepresentinLiverpool,andobtainedaseculartrendforheperiodupto 1 880of0 .39± 0 .1 7mm a ,andatrendfort…     

Hilbert Transferin Applied to Separation of Waves

The analytical method (AM) for separation of composite waves is presented based on the Hilbert transform. It is ap-plicable to both regular and irregular trains of waves. The wave data series measured with two wave gauges in the experi-ments are separated into two series of incident and reflected waves. Then, the reflection coefficient can be easily ob-tained. The arrival of reflected waves can also be detected for improveraent of the accuracy of the reflection coefficient. The reflection performance of the physical model can be estimated exactly without calculation of wave height and phase difference. Numerical samples developed to test the method are proved to be accurate. Physical experiments are conduct-ed and compared with Goda s method and satisfactory results are obtained.     

Estimation of Water Particle Velocities by Empirical Transfer Function

-In previous and this studies it appears that the linear and nonlinear wave theory can notaccurately and easily predict the water particle velocities.Therefore,different from the theoretical consider-ations,in this study we have attempted to determine the transfer function empirically.Laboratory experi-ments were performed under various wave conditions.The empirical formulas of the transfer function ofthe wave height,angular frequency and water particle velocity were obtained on the basis of these test databy dimensional analysis and regression analysis.In intermediate and deep water depth conditions,thetransfer function was only a function of a nondimensional parameter which is composed of the angular fre-quency,the depth of the velocity gauge under the still water level,water depth and the acceleration of grav-ity.Finally,the empirical formulas were compared with experimental data and observational data formpresent and Cavaleri's(1978)studies.The empirical formulas were found to be in sufficient correl     

Nonlinear Static Finite Element Stress Analysis of Pipe-in-Pipe Risers

Owing to the complexity of the pipe-in-pipe (PIP) riser system in structure, load and restraint, many problems arise in the structural analysis of the system. This paper presents a new method for nonlinear static finite element stress analysis of the PIP riser system. The finite element (FE) model of the PIP riser system is built via software AutoPIPE 6.1. According to the specialties of a variety of components in the PIP riser system, different elements are used so as to model the system accurately. Allowing for the complication in modeling the effects of seabed restraint, a technique based on the bilinear spring concept is developed to calculate the soil properties. Then, based on a pipeline project, the entire procedure of stress analysis is discussed in detail, including creation of an FE model, processing of input data and analysis of results. A wide range of loading schemes is investigated to ascertain that the stresses remain within the acceptable range of the pipe material strength. Finally, the effects of the location of flanges, the thermal expansion of submarine pipelines and the seabed restraint on stress distribution in the riser and expansion loop are studied, which are valuable for pipeline designers.     

Stratigraphic framework of gas accumulations in the Ría de Pontevedra (NW Spain)

Knowledge of marine geological environments in which shallow gas is accumulating is becoming increasingly important in global studies of climate change because a measurable proportion of the total methane source comes from continental margins. Previous studies have revealed that coastal environments represent important geological environments where microbial methane is being generated, is accumulating, and is being released. In the Ría de Pontevedra, at least 4.5 km² of seafloor in the innermost part of the ría is underlain by sediments containing natural gas. Seismic interpretation contributes new findings for the definition of periods and geological environments in which the gas could have been generated, and is accumulating and released in the Ría de Pontevedra. Groundtruthing the seismic data (facies, environments) makes it possible to identify favourable geological environments for gas generation in the sedimentary infill of the Ría de Pontevedra. Sequence stratigraphy based on high-resolution seismic profiles and post-Last Glacial Maximum sea-level records makes it possible to establish the stratigraphic architecture of the ría and to define the periods in which gas could have been generated. The results of this study show that the sedimentary infill is composed of a fifth-order sequence developed since the Last Glacial Maximum. Within this sequence, gas appears to have accumulated in the Holocene deposits associated with the latest transgressive and highstand system tracts. Seismic analysis shows that gas could have been generated in different geological environments in the Ría de Pontevedra. If coastal environments at times of lower sea level were similar to those of the present, organic-rich mud deposits (deposited mainly in lakes, estuaries and floodplains) could have survived transgression and remained buried as potential gas sources in the inner part of the ría.     

Numerical Simulation of Non-Linear Wave Propagation in Waters of Mildly Varying Topography with Complicated Boundary

In this paper, the characteristics of different forms of mild slope equations for non-linear wave are analyzed, and new non-linear theoretic models for wave propagation are presented, with non-linear terms added to the mild slope equations for non-stationary linear waves and dissipative effects considered. Numerical simulation models are developed of non-linear wave propagation for waters of mildly varying topography with complicated boundary, and the effects are studied of different non-linear corrections on calculation results of extended mild slope equations. Systematical numerical simulation tests show that the present models can effectively reflect non-linear effects.     

Early Uplift History of the Tibetan Plateau:Records from Paleocurrents and Paleodrainage in the Hoh Xil Basin

Paleocurrent indicator data collected in field work were used to study the early Cenozoic regional paleodrainage patterns in the Hob Xil basin in northern Tibetan plateau. The paleocurrent directions of the Eocene Fenghuoshan Group obviously show that the flows were northward with a unidirectional dispersal pattern. This probably reflects the uplift of the Qiangtang terrain during the initial basin deposition period and indicates that the Tanggula Moutains occurred as topographic highlands at least in the Eocene. Paleoflows of the Oligocene Yaxicuo Group were dominantly oriented to the north and then flowed eastwards during its late deposition. This regional variability of paleodrainage patterns of the Yaxicuo Group is interpreted to record the dispersal style of sediments from transverse rivers to longitudinal river systems. It is inferred that the Oligocene uplift of the Kunlun Mountains obstructed by northward paleoflows and created longitudinal river systems parallel to the orogenic belts. The temporal and spatial changes of the paleodrainage patterns suggest that the northern boundary of the Tibetan plateau during the early Cenozoic was situated in the Hoh Xil area and its uplift has progressed northwards through time.