Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

An improved ionosphere interpolation algorithm for network RTK in low-latitude regions

A traditional interpolation algorithm with the linear interpolation method (LIM) using a fixed number of reference stations is widely used in network RTK to obtain the ionospheric delays for the users. In low-latitude regions, where the ionosphere is relatively active, however, large interpolation errors exist, especially for satellites at low elevation angles. Considering the characteristics of “coinciding ionospheric pierce points (CIPPs)” with a similar nature of ionospheric delays, an improved interpolation algorithm is proposed. In this algorithm, all stations with CIPPs are used to establish the interpolation model; thus, more precise interpolation model is achieved. To validate the performance of the proposed algorithm, data from some reference stations in Guangdong Province of China were used, and the results are compared with those with the traditional interpolation algorithm. Numerical analysis shows that the interpolation accuracy of the proposed algorithm increases by 10–30% compared with the traditional one. Since the number of reference stations is flexible, the proposed algorithm can also balance the model accuracy with the computation burdens. In addition, the proposed algorithm is less affected by the selection of master reference station. In terms of network RTK on-the-fly positioning, the time-to-first-fix is reduced when replacing the traditional interpolation algorithm with the proposed one.     

The nonlinear time lag multivariable grey prediction model based on interval grey numbers and its application

Natural Hazards - The linear relationship of the original grey prediction model is too single, and the original grey prediction model does not consider the time delay of the effect of the current...     

基于孔压静力触探的岩土参数正态性检验方法对比

岩土参数的正态分布是岩土工程可靠分析的基本假定之一。目前,岩土工程参数的正态性检验以KS （Kolmogorov-Smirnov）检验为主,然而诸多研究表明,对于小样本数据,KS检验的正态性检验结果并不可靠。本文以废黄河泛滥沉积相粉土中的孔压静力触探（CPTU）锥尖阻力q_t数据为研究对象,首先采用ANOVA （方差分析）检验,从CPTU测试资料中进行总体的样本筛选,然后应用SW （Shapiro-Wilk）检验、KS检验、LF （Lilliefors）检验和AD （Anderson-Darling）检验等4种正态性检验方法,在不同样本容量条件下,对测试数据进行正态性检验评价。当测试数据的样本容量充足时,分析结果表明：SW检验结果最为严格,KS检验结果最不保守,而LF检验和AD检验的严格性介于两者之间;对于常规岩土工程设计,采用KS检验结果即可满足稳定性分析要求,对于复杂岩土工程设计,采用SW检验对设计参数进行正态性检验,可降低设计的不确定性。     

Machine learning reveals the influences of grain morphology on grain crushing strength

Acta Geotechnica - Grain morphology has significant impacts on the mechanical behaviors of granular materials. However, its influences on grain breakage are still poorly understood due to the...     

地磁Z分量低点时间季节性变化研究

通过小波分析和频谱分析,发现Z分量低点时间的小波变换结果曲线(α=6)明显地随节气而变化;对地磁Z分量低点时间季节性变化进行了初步的比较分析.