南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

Lateral dynamic response of a partially embedded pile subjected to combined loads in saturated soil

In this article, an analytical solution is proposed to investigate the lateral dynamic response of a pile which is partially embedded in saturated soil layer and subjected to combined lateral and vertical loads. The saturated soil is described by Biot’s poroelastic theory and the resistance of soil is derived by potential function method. The governing equation of the pile is solved by coupling soil resistance and continuity conditions between the pile and the soil. The dynamic impedances of the pile are then obtained through transfer matrix method. To verify the validity of the proposed procedure, the present solution is compared with available solution for an idealized case. Finally, a parametric study is performed to investigate the effects of various parameters on the stiffness and damping properties of the pile-soil system. It is found that permeability of the soil and vertical load has significant effects on the dynamic response of the pile.     

Effect of Groundwater Fluctuations on Pore Pressures and Earth Pressures on Coastal Excavation Retaining Walls

Pore water and earth pressures acting on retaining structures are investigated using an efficient coastal double-layered excavation model to determine offshore excavation responses to groundwater fluctuations outside foundation pits. Total pore water pressure includes excess pore water pressure (due to groundwater fluctuations) and steady pore water pressure (due to steady seepage) determined using one-dimensional consolidation theory of double-layered soil and one-dimensional steady-state flow theory, respectively. Rankine's active and passive earth pressures are obtained from pore water pressure. This method is applicable to arbitrary groundwater fluctuation conditions. How physical parameters affect pore water pressure is numerically investigated using examples, demonstrating the method's practicality for calculating pore water and earth pressures.     

Self-Weight Penetration Characteristics of the Suction Foundation with Different Diameters in Sand

During the self-weight penetration process of the suction foundation on the dense sand seabed, due to the shallow penetration depth, the excess seepage seawater from the outside to the inside of the foundation may cause the negative pressure penetration process failure. Increasing the self-weight penetration depth has become an important problem for the safe construction of the suction foundation. The new suction anchor foundation has been proposed, and the self-weight penetration characteristics of the traditional suction foundation and the new suction anchor foundation are studied and compared through laboratory experiments and analysis. For the above two foundation types, by considering five foundation diameters and two bottom shapes, 20 models are tested with the same penetration energy. The effects of different foundation diameters on the penetration depth, the soil plug characteristics, and the surrounding sand layer are studied. The results show that the penetration depth of the new suction foundation is smaller than that of the traditional suction foundation. With the same penetration energy, the penetration depth of the suction foundation becomes shallower as the diameter increases. The smaller the diameter of the suction foundation, the more likely it is to be fully plugged, and the smaller the height of the soil plug will be. In the stage of self-weight penetration, the impact cavity appears around the foundation, which may affect the stability of the suction foundation.

     

基于网络药理学和分子对接技术探究柯里拉京治疗卵巢癌的关键靶点及分子机制

目的：采用网络药理学和分子对接技术相结合的方法探究柯里拉京（Cor）治疗卵巢癌的关键作用靶点和分子机制。方法：利用PharmMapper、TargetNet及Swiss Target Prediction在线工具预测Cor的作用靶点,通过OMIM、TTD、CTD、DisGenet、PharmGKB及MalaCards数据库获取卵巢癌相关的疾病靶点,并将药物靶点与疾病靶点取交集得到Cor治疗卵巢癌的预测靶点。借助STRING数据库、Cytoscape 软件构建蛋白质-蛋白质相互作用（PPI）网络,并采用CentiScape插件筛选出PPI网络中的核心靶点。运用GEPIA和Kaplan-Meier Plotter在线平台分别对这些核心靶点进行基因表达差异分析和生存分析,以明确Cor治疗卵巢癌的关键靶点。利用DAVID数据库进行基因本体（GO）功能及京都基因与基因组百科全书（KEGG）通路富集分析。运用Autodock Vina软件进行正向分子对接,预测关键靶点和Cor之间的结合活性。结果：Cor治疗卵巢癌的预测作用靶点共24个,其中RAC-α丝氨酸/苏氨酸蛋白激酶(AKT1)、表皮生长因子受体(EGFR)、雌激素受体1(ESR1)、原癌基因酪氨酸蛋白激酶(SRC)是Cor治疗卵巢癌的关键靶点,主要涉及血管内皮生长因子(VEGF)信号通路、缺氧诱导因子-1(HIF-1)信号通路及叉头样转录因子(FoxO)信号通路,可能通过调控细胞凋亡、细胞增殖、血管内皮细胞迁移等生物过程而发挥治疗卵巢癌的作用。分子对接结果显示,Cor与预测靶点之间具有较好的亲和力,表明通过网络药理学预测作用靶点的方法具有良好的可靠性。结论：Cor是通过多靶点、多通路的协同作用来干预多个生物过程,从而发挥其抗卵巢癌疗效,为进一步研究其治疗卵巢癌的分子机制提供了新的线索。     

石榴石橄榄岩的熔融实验及其地质意义

在20×10~9Pa和25×10~9Pa条件下,对石榴石橄榄岩进行的14次高温高压熔融实验研究表明,在1600℃时石榴石橄榄岩开始熔融(初熔),熔体中的SiO_2>70％,Na_2O+K_2O>8％,类似于A型花岗岩成分。本实验为地幔花岗岩的成因提供了证据。     

中国海相探矿权区块定量评价方法

在研究了我国海相油气探矿权区块基本油气地质条件、勘探程度、技术经济条件等要素和特点的基础上,给出了48个评价参数和归一化取值参照表,建立了探矿权区块评价工作流程、评分参数及计算公式。提出对我国海相勘探层探矿权区块评价应该注意:与国外海相碳酸盐岩评价的差别;与国内陆相碎屑岩评价的差别;成藏组合的划分;评价内容的有效性、不确定性和评价参数的可信度;成败经验的总结和勘探程度的研究。     

澜沧—耿马地震震源动力学参数研究

本文使用沧澜—耿马地震主震后在震区建立的数字地震仪记录,用波谱法测算澜沧—耿马余震的震源动力学参数。就这些参数的统计特征,对该双主震型两主震区的地下介质不同状态予以讨论。     

The roles of different mechanisms related to the tide-induced fronts in the Yellow Sea in summer

In summer, the Yellow Sea Cold Water Mass （YSCWM） is a stable water mass of low temperature lying at the bottom of the central Yellow Sea （YS）. It is fringed by some typical tidal fronts, which separate deep, stratified water on the offshore side from the well-mixed, shallow water on the inshore side. Three striking fronts--Subei Bank Front （SBF）, Shandong Peninsula Front （SPF）, and Mokpo Front （MKF; a front off the southwestern tip of the Korean Peninsula）--have been iden- tified by various studies from both satellite observations and model results. Tide plays an important role in the formation and maintenance of these fronts. However, it is still a matter of debate as to the roles these two kinds of mechanisms of upwelling and tidal mixing play, and how importance they are in the maintenance processes of the above three fronts. Basing a nested high-resolution model HYCOM （the Hybrid Coordinate Ocean Model）, this study focuses on the different mechanisms of tidal effects on the thermal fronts in the YS in summertime. Through comparative experiments with and without tidal forcing, the results indicate that the MKF is mainly driven by tide-induced upwelling. For the SPF, tidal mixing is the dominant factor, when lower cold water is stirred upwards along the sloping topography of the western YS. Meanwhile, the combined effect of upwelling and tidal mixing is the main cause of the formation of the SBF. Diagnostic analysis of thermal balance shows that horizontal nonlinear advection induced by strong tidal currents also contributes to the thermal balance of frontal areas.     

9210工程地市(县)级数据传输处理本地化的若干问题

经过一年多的业务运行，我们发现MICAPS系统在数 据传输处理 中存在不尽完善的地方，造成数值预报产品残缺不全；由于数据传输处理未作配置本地化或 配置本地化不完善，致使数值预报产品和实况资料的分析范围、等值线间距、时效、层次等 不能满足本地预报业务工作的需要。针对上述存在的问题，本文介绍了资料传输、处理有关 配置文本本地化的方法。使数值预报产品有用率在原有基础上增加了约一倍。