Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization

107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.     

塔里木盆地寒武系烃源岩分布的重新认识及其意义

塔里木盆地台盆区海相烃源岩的分布是制约油气勘探的主控因素,在构造—古地理背景分析基础上,通过典型井地球化学分析结合测井标定与地震追踪,重新厘定寒武系主力烃源岩分布。结果表明,东部烃源岩主要分布在中-下寒武统泥页岩中,西部烃源岩主要分布在下寒武统泥页岩中,中-上寒武统—下奥陶统碳酸盐岩缺乏有效烃源岩,单井纵向厚度标定东部低于100 m、西部低于40 m,远低于早期预测厚度。寒武系烃源岩并非满盆分布,主要分布在满东凹陷、满西台内洼,并发现塔西南南缘可能发育大面积陆缘斜坡烃源岩,而巴楚—麦盖提、塘古—塔中、塔北等基底古隆起区缺乏烃源岩。受控前寒武纪古隆起与寒武纪弱伸展背景影响,寒武系发育4种构造背景下的烃源岩：主要为台间盆、台内洼烃源岩,以及可能存在被动陆缘斜坡、板块边缘裂谷烃源岩。     

卸荷-增孔压条件下花岗岩残积土的力学特性

建（构）筑物因土中孔隙水压力升高而失稳破坏的情形屡见不鲜,常剪应力增孔压试验是相关研究的常用手段,但迄今鲜见考虑施工期开挖卸荷影响的试验研究。为探究初始卸荷对花岗岩残积土增孔压过程力学行为的影响,在常规增孔压试验中增加了初始卸荷阶段,开展了一系列考虑初始卸荷程度和卸荷滞时影响的卸荷-增孔压试验,分析土体变形、屈服和强度特性的初始卸荷效应与卸荷滞时效应,并与常规增孔压试验结果对比以揭示初始应力路径的影响。试验结果表明：增孔压过程的变形发展具有明显的阶段性,土体屈服后因其剪胀潜势和变形迅速而产生负超静孔压增量,有效平均应力转而增大;初始卸荷对增孔压过程土体力学性能具有明显的劣化作用,表现为刚度与屈服强度均随初始卸荷程度的增大而衰减;与被动压缩这种“加荷”式应力路径相比,初始卸荷的不利影响更为显著;土体力学性能的劣化随卸荷滞时的增长而加剧;屈服摩擦角随初始卸荷程度的增大逐渐减小,但始终大于临界状态摩擦角。     

大数据环境下内蒙古浩布高地区铅锌多金属矿智能矿产预测研究

传统机器学习算法已广泛应用于矿产预测,但面对地质大数据的高维稀疏、不平衡小样本等特性仍缺乏有效处理和分析的方法,设计适合地质大数据特点的机器学习算法是智能矿产预测亟需解决的新问题。本文以内蒙古浩布高地区的铅锌多金属矿产预测为例,提出了一种面向地质大数据的半监督协同训练矿产预测模型。首先对研究区地质找矿信息和地球化学异常信息进行定量分析,提取断裂构造、二叠系地层、燕山期侵入岩、地层与岩体接触带、围岩蚀变及Pb、Zn、Sn、Cu地球化学异常共9种找矿因子。然后利用递归特征消除法优选找矿因子组合,不包括Sn异常在内的8个找矿因子组合被选为最优组合。最后,利用支持向量机和随机森林算法作为基分类器进行半监督协同训练矿产预测,绘制成矿概率分布图。ROC曲线和预测度曲线分析结果表明,半监督协同训练模型的AUC值和预测效率都高于随机森林和支持向量机模型。研究结果也为大数据环境下的智能矿产预测提供了一种新的思路。     

Post-earthquake economic resilience and recovery efficiency in the border areas of the Tibetan Plateau: A case study of areas affected by the Wenchuan Ms 8.0 Earthquake in Sichuan,China in 2008

Journal of Geographical Sciences - The border areas of the Tibetan Plateau and the neighboring mountainous areas have a high incidence of earthquakes with a magnitude greater than Ms 5.0, as well...     

Rapid forecasting of urban flood inundation using multiple machine learning models

Natural Hazards - Urban flood inundation is worsening as the number of short-duration rainstorms increases, and it is difficult to accurately predict urban flood inundation over a long lead time;...     

藻类功能食品的物质基础与研究开发现状

对藻类功能食品的研究和开发现状作综述报道。涉及的主要内容有：作为功能食品物质基础的食物纤维、ＰＵＦＡ、维生素、矿物质和微量元素、氨基酸以及其它有生理活性的物质。     

NEW OBSERVATIONS ON THE MEIOTIC PROCESS IN THE MARINE DINOFLAGELLATE NOCTILUCA SCINTILIANS （NOCTILUCALES, DINOPHYCEAE）

The meiotic process in Noctiluca scintillans were observed under light microscope.Some abnormal cell divisions, incompletely separated “zoospores” and the changes of the zoospores are described in this paper. Together with the fmdings of field samplings and the previous results by other researchers, the process of meiosis in N. scintillans was supposed to be a pathway to reduce the extra high density of NH3-N within the cell in order to ensure normal population growth.     

元岭金矿蚀变破碎带特征及其找矿意义

根据对元岭金矿三条蚀变破碎带一般地质特征、微量元素特征的对比研究，并借鉴邻近矿区蚀变破碎带的矿化特征，本文阐明了元岭蚀变破碎带矿化的有利构造部位，总结了蚀变破碎带特征与矿化强度之间的关系，指出蚀变破碎带的矿化蚀变元素为中低温组合，矿化蚀变愈强则参与元素的种类愈多、数量愈大。围岩性质对矿化蚀变有一定影响，围岩中的易迁移亲铜元素和亲硫铁族无素对矿化有利。据此，为进一步成矿预测、评价蚀变破碎带的含矿性提供依据。     

晋西北地区表层土壤粒度与地球化学元素组成

对晋西北地区表层土壤的物质组成进行研究,可以明确其物质来源、沉积环境及化学风化特征。选择区内表层土壤（深度0、10、20 cm）及其附近的河流沉积物,进行粒度和化学元素组成测试。结果表明：（1）晋西北地区表层土壤粒度组成以黏土和粉沙为主,且有自地表向下粗颗粒组分含量增加的趋势。（2）常量化学元素组成以SiO₂、Al₂O₃、CaO、Fe₂O₃为主,其余元素含量均较低。化学元素组成模式表明研究区内表层土壤物质与黄土高原腹地黄土有相同的物质源区,黄河河流沉积物对其贡献不大。（3）晋西北表层土壤的CIA值为52.70~57.89,平均54.06,说明它们的化学风化程度较低,处于早期的脱Na、Ca阶段。这是由于研究区地理位置比洛川更靠北,气温和降水量均低于洛川,降水量与兰州相差不大,但是年平均气温和年温差比兰州低。该研究明确了晋西北地区表层土壤的物质组成特征,为区域潜在沙漠化危害防治提供了数据支撑。