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Survey of cyanomyovirus abundance in Shantou coastal waters by g20

To understand the genetic diversity and population changes in cyanophages in the coastal waters of Shantou,northeast South China Sea,we used the capsid assembly protein gene g 20 as a marker of the abundance and phylogeny of natural cyanomyovirus communities. The abundance of total viruses,heterotrophic bacteria,and picophytoplankton in the coastal waters was monitored with flow cytometry. Hydrological parameters(NO ˉ 3,NO ˉ 2,NH 3,soluble reactive phosphorus,total dissolved nitrogen,total dissolved phosphorus,dissolved oxygen,chemical oxygen demand,temperature,salinity,and chlorophyll a concentration) and microbial abundance(total viruses,total bacteria,P rochlorococcus,Synechococcus,and eukaryotes) were measured in the upper and lower layers at four sampling sites in the research area. In the direct viral counts,cyanomyoviruses accounted for 1.92% to 10% of the total viral community. A phylogenetic analysis showed that the g 20 sequences in the Shantou coastal waters were very diverse,distributed in eight distinct operational taxonomic units,including the newly formed Cluster W. The g 20 gene copies inferred from real time PCR assay indicated that cyanomyoviruses were correlated significantly with the heterotrophic bacteria numbers and the nitrate and chlorophyll a concentrations. These results suggest that cyanomyoviruses are ubiquitous and are an abundant component of the virioplankton in Shantou coastal waters.     

伶鼬榧螺(Oliva mustelina)的分子鉴定及其形态变异

根据榧螺螺旋部的差异可将其分为两种不同的形态类型,类型Ⅰ(MorphotypeⅠ)的个体螺旋部陷入体螺层中,其壳顶与前部数螺层愈合;类型Ⅱ(MorphotypeⅡ)的个体螺旋部高出壳顶,缝合线明显,此类型可明确鉴定为伶鼬榧螺(Oliva mustelina)。本研究通过线粒体COⅠ和16SrRNA基因序列的分析对两种形态类型的榧螺进行了分子鉴定。结果表明:两种形态类型的榧螺含有共享的单倍型;不同形态类型间的遗传距离和同一形态类型内的遗传距离重叠;两者的单系性在系统发育分析中没有得到显现,而是共同构成一个单系。因此,两种形态类型的个体均为伶鼬榧螺。类型Ⅰ可能是伶鼬榧螺中一种少见的有别于典型个体的形态变异类型。     

HY-2A卫星雷达高度计数据的全球统计评价及质量分析

自HY-2A卫星发射以来,针对HY-2A卫星雷达高度计产品的交叉定标、真实性检验及质量评估工作一直在持续开展。本文主要以HY-2A卫星高度计第44周期的IGDR产品数据为例,通过使用全球分布图、二维直方图和每日均值统计的方法完成了与Jason-2IGDR产品的比对验证,同时对主要环境校正参数及地球物理产品的数据质量稳定性进行了分析,结果显示高度计产品数据质量较稳定,此外利用HY-2A卫星升降轨交叉点海面高度差、与Jason-2卫星交叉点海面高度差以及沿轨海平面异常数据分析的方法进行了HY-2A卫星高度计观测系统的性能评估,结果显示,HY-2A卫星海面高度精度约为7.48cm,精度接近Jason-2,能满足海洋应用与科学研究的需要。     

区域海气耦合模式FROALS模拟的西北太平洋环流及其年际变率

本文利用区域海气耦合模式FROALS(Flexible Regional Ocean-Atmosphere-Land System)对西北太平洋地区1984-2007年连续积分结果,对比SODA(Simple Ocean Data Assimilation)同化资料讨论了西北太平洋海表温度和表层洋流的气候态及年际变率。结果表明,FROALS基本能够再现冬、夏季季节平均的海温型,但均存在一个明显的冷偏差;FROALS对气候平均态的表层洋流有较高的模拟技巧,对于冬、夏季的表层洋流型都能够再现。另外,表层洋流的模拟偏差与海表高度的模拟偏差直接相关。由于模式模拟的黑潮热输送较观测偏强,使得模式模拟的海洋热输送倾向于使黑潮路径上的海温呈现正偏差。从表层洋流的年际变率来看,模式模拟的与ENSO(El Nio-South Oscillation)相联系的年际变率信号与观测相似:在El Nio年,北赤道流和棉兰老流增强,低纬度西太平洋海表高度降低,而在La Nia年则呈现出相反的形态,但是在模式中这种信号稍强于观测。     

Proline with or without hydroxyproline influences collagen concentration and regulates prolyl 4-hydroxylase α (I) gene expression in juvenile turbo (Scophthalmus maximus L.)

Journal of Ocean University of China - This study was conducted to investigate the effect of dietary proline (Pro), and Pro and hydroxyproline (Hyp) in combination on the growth performance, total...     

Effects of waterborne Cu and Cd on anti-oxidative response,lipid peroxidation and heavy metals accumulation in abalone Haliotis discus hannai ino

The aim of this study was to compare the effects of waterborne copper(Cu) and cadmium(Cd) on survival, anti-oxidative response, lipid peroxidation and metal accumulation in abalone Haliotis discus hannai. Experimental animals(initial weight: 7.49 g ± 0.01 g) were exposed to graded concentrations of waterborne Cu(0.02, 0.04, 0.06, 0.08 mg L-1) or Cd(0.025, 0.05, 0.25, 0.5mg L-1) for 28 days, respectively. Activities of the anti-oxidative enzymes(catalase, CAT; superoxide dismutase, SOD; glutathione peroxidases, GPx; glutathione S-transferase, GST), contents of the reduced glutathione(GSH) and malondiadehyde(MDA) in the hepatopancreas, and metal accumulation in hepatopancreas and muscles were analyzed after 0, 1, 3, 6, 10, 15, 21, 28 days of metal exposure, respectively. Results showed that 0.04 mg L-1, 0.06 mg L-1 and 0.08 mg L-1 Cu caused 100% death of abalone on the 21 st, 10 th and 6th day, respectively. However, no dead abalone was found during the 28-day waterborne Cd exposure at all experimental concentrations. Generally, activities of SOD and GST in hepatopancreas under all Cu concentrations followed a decrease trend as the exposure time prolonged. However, these activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Activities of CAT in all Cu exposure treatments were higher than those in the control. These activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Contents of MDA in hepatopancreas in all Cu treatments significantly increased first and then decreased to the control level. However, the MDA contents in hepatopancreas were not significantly changed during the 28-day Cd exposure. The metals accumulation in both hepatopancreas and muscles of abalone significantly increased with the increase of waterborne metals concentration and exposure time. These results indicated that H. discus hannai has a positive anti-oxidative defense against Cu or Cd. In conclusion, anti-oxidative mechanism in abalone to resist waterborne Cu did not follow the same pattern as that for waterborne Cd.     

鄂尔多斯盆地奥陶系马家沟组四段构造- 岩相古地理新认识及勘探意义

米探1井取得天然气勘探突破表明鄂尔多斯盆地奥陶系马家沟组四段具有巨大勘探潜力,然而,对盆地奥陶纪马四期古构造格局和岩相古地理研究还存在争议,制约了下步天然气勘探进程。为此,基于地震、野外露头、钻井、岩芯、微观薄片和测井等基础资料,对马家沟组四段构造- 岩相古地理开展了系统研究,确定了盆地奥陶纪马四期古构造格局,并恢复出马家沟组四段岩相古地理,预测了有利勘探区。研究结果表明：① 鄂尔多斯盆地奥陶纪马四期发育“两陆三隆四坳”的古构造格局,其中两陆为伊盟古陆和阿拉善古陆,三隆分别为中央古隆起、中条古隆起和神木- 志丹低隆起,四坳为西南边缘坳陷、桃利庙坳陷、府谷坳陷和柳林坳陷;② 马四期为碳酸盐岩内缓坡和中缓坡沉积,其中内缓坡又发育白云岩坪、含云膏坪、微生物丘、灰泥丘、砂屑滩、丘滩间海和灰质潟湖等7类微相;③ 古构造格局控制马家沟组四段沉积分异,微生物丘、灰泥丘和砂屑滩等3类有利储集相主要沿中央古隆起和神木- 志丹低隆起等古地貌高部位分布。因此认为,神木—靖边地区是有利储层发育区,且处于岩性相变带,生储盖配置关系好,具有良好的成藏条件,是天然气勘探的有利靶向区。     

An experimental study of effect of pre‐existing fabric on deformation of foliated mylonite at high temperature and pressure

We performed deformation experiments on a foliated mylonite under high temperature and pressure conditions in this study. To investigate the effect of pre‐existing fabric on the rheology of rocks, our samples were drilled from natural mylonite with the cylinder axis parallel to the foliation (PAR) and perpendicular to the foliation (PER). We performed 25 tests on seven PAR samples and 21 tests on seven PER samples at temperatures ranging from 600 to 890 °C, confining pressures ranging from 800 to 1400 MPa, and steady‐state strain rates of 1 × 10⁻⁴, 1 × 10⁻⁵ and 2.5 × 10⁻⁶ s⁻¹. In the temperatures of 600–700 °C, the deformation is accommodated by semi‐brittle flow, with the average stress exponent being 6–7 assuming power law flow; in the temperature range of 800–890 °C, deformation is mainly by plastic flow, with an average stress exponent of n = 3 and activation energies of Q = 354 ± 52 kJ/mol (PER and PAR samples). The experimental results show that the strengths of PER samples are higher than those of PAR samples. Deformation microstructures have been studied by optical and electron microscopy. The original foliation of PER samples is destroyed by deformation and replaced by a new foliation, but the deformation of PAR samples followed the original foliation. Electron backscatter diffraction (EBSD) measurements show a strong lattice preferred orientation (LPO) of the quartz c axis fabrics of the starting samples and deformed PER and PAR samples. However, the c axis fabric of quartz in experimentally deformed PER and PAR samples varied with temperature and strain rate is different from that seen in the starting mylonite sample. The initial quartz c axis fabric of the starting mylonite sample has been transformed into a new fabric during experimental deformation. Dehydration melting of biotite and hornblende occurred in both PER and PAR samples at temperatures of 800–890 °C. Copyright © 2014 John Wiley & Sons, Ltd.