Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Experimental research on the effect of slope morphology on bank collapse in mountain reservoir

Farmers along the Amazon River each year face multiple natural hazards that threaten crop production and limit the potential for agricultural development of the expansive floodplain and active channel. In this paper we report the findings of a study of natural hazard-related risk associated with rice production on silt bars in the active channel of the Amazon River near Iquitos, Peru. Data were gathered in four rice producing communities in 2014 using household surveys (n = 83 households), focus group discussions, surveying of land elevations along the Amazon River, and interpretation of remote sensing imagery. The probability, extent, and severity of rice crop shortfalls were estimated for recent production years and the economic losses to farming households were also assessed. Our findings point to a very high risk of crop shortfalls due to natural hazards, suggesting that a good year brings rice farmers bounty and a bad year, near penury. River stage reversals (repiquetes) and edaphic conditions were found to be more problematic than the often cited hazard of high and/or early floods. Also surprisingly, farmers’ perceptions of hazards and risk diverged markedly from actual shortfalls experienced during the production years studied. Our results provide the first quantitative estimates of risk due to the multiple natural hazards along the Amazon River and point to the need to assist lowland farmers with risk mitigation so as to unlock the considerable potential of Amazon floodlands for agricultural production.     

铜陵新桥层状菱铁矿成因的矿物学证据及成矿意义

安徽铜陵新桥矿区二叠系栖霞组底部和石炭系黄龙组—船山组之间产出层状、似层状菱铁矿矿层。开展菱铁矿矿层成因研究对于深入剖析区域层控矽卡岩型铜铁矿床成矿机制具有重要意义。本文利用粉晶X射线衍射（XRD）、扫描电镜（SEM）对菱铁矿矿石进行矿物学研究,结果发现菱铁矿矿石主要由菱铁矿、石英、伊利石和有机质等组成,菱铁矿颗粒粒径较小,表面具有成岩自生的自形石英硬模的微结构,SEM原位微区成分分析显示菱铁矿中除了主量元素铁,还含有大量的锰、锌和钙。矿石中存在两种微结构和不同成因的石英：表面具菱铁矿硬模和次生加大结构的碎屑石英;具六方双锥、单锥以及生物成因球形的自生石英。菱铁矿矿石的组成和矿物表面微结构表明其为沉积成因,非岩浆热液起源。富有机质和亚铁的沉积菱铁矿层和沉积胶状黄铁矿层协同作用,可能是铜陵地区乃至长江中下游成矿带层状铜铁矿床层控性重要制约因素,以及可能作为燕山期中酸岩浆演化的氧化性含铜成矿流体卸载成矿的地球化学还原障。

     

Climatic and geographic factors affect ecosystem multifunctionality through biodiversity in the Tibetan alpine grasslands

Ecosystem multifunctionality(EMF), the simultaneous provision of multiple ecosystem functions, is often affected by biodiversity and environmental factors. We know little about how the interactions between biodiversity and environmental factors affect EMF. In this case study, a structural equation model was used to clarify climatic and geographic pathways that affect EMF by varying biodiversity in the Tibetan alpine grasslands. In addition to services related to carbon, nitrogen, and water cycling, forage supply, which is related to plantproductivity and palatability, was included in the EMF index. The results showed that 72% of the variation in EMF could be explained by biodiversity and other environmental factors. The ratio of palatable richness to all species richness explained 8.3% of the EMF variation. We found that air temperature, elevation, and latitude all affected EMF, but in different ways. Air temperature and elevation impacted the aboveground parts of the ecosystem, which included plant height, aboveground biomass, richness of palatable species, and ratio of palatable richness to all species richness. Latitude affected EMF by varying both aboveground and belowground parts of the ecosystem, which included palatable speciesrichness and belowground biomass. Our results indicated that there are still uncertainties in the biodiversity–EMF relationships related to the variable components of EMF, and climatic and geographic factors. Clarification of pathways that affect EMF using structural equation modeling techniques could elucidate the mechanisms by which environmental changes affect EMF.     

Dark energy model in anisotropic Bianchi type-III space-time with variable EoS parameter

A new dark energy model in anisotropic Bianchi type-III space-time with variable equation of state (EoS) parameter has been investigated in the present paper. To get the deterministic model, we consider that the expansion θ in the model is proportional to the eigen value s² ₂\sigma^{2}_{~2} of the shear tensor s^j _i\sigma^{j}_{~i}. The EoS parameter ω is found to be time dependent and its existing range for this model is in good agreement with the recent observations of SNe Ia data (Knop et al. in Astrophys. J. 598:102, 2003) and SNe Ia data with CMBR anisotropy and galaxy clustering statistics (Tegmark et al. in Astrophys. J. 606:702, 2004). It has been suggested that the dark energy that explains the observed accelerating expansion of the universe may arise due to the contribution to the vacuum energy of the EoS in a time dependent background. Some physical aspects of dark energy model are also discussed.     

Analysis of spatial clustering optimization

Spatial clustering is widely used in many fields such as WSN (Wireless Sensor Networks), web clustering, remote sensing and so on for discovery groups and to identify interesting distributions in the underlying database. By discussing the relationships between the optimal clustering and the initial seeds, a clustering validity index and the principle of seeking initial seeds were proposed, and on this principle we recommend an initial seed-seeking strategy: SSPG (Single-Shortest-Path Graph). With SSPG strategy used in clustering algorithms, we find that the result of clustering is optimized with more probability. At the end of the paper, according to the combinational theory of optimization, a method is proposed to obtain optimal reference k value of cluster number, and is proven to be efficient.     

The Data Depth-weight-kernel Estimation of the Model Error of Satellite Orbit Determination

It is an objective fact that there exists error in the satellite dynamic model and it will be transferred to satellite orbit determination algorithm, forming a part of the connotative model error. Mixed with the systematic error and random error of the measurements, they form the unitive model error and badly restrict the precision of the orbit determination. We deduce in detail the equations of orbit improvement for a system with dynamic model error, construct the parametric model for the explicit part of the model and nonparametric model for the error that can not be explicitly described. We also construct the partially linear orbit determination model, estimate and fit the model error using a two-stage estimation and a kernel function estimation, and finally make the corresponding compensation in the orbit determination. Beginning from the data depth theory, a data depth weight kernel estimator for model error is proposed for the sake of promoting the steadiness of model error estimation. Simulation experiments of SBSS are performed. The results show clearly that the model error is one of the most important effects that will influence the precision of the orbit determination. The kernel function method can effectively estimate the model error, with the window width as a major restrict parameter. A data depth-weight-kernel estimation, however, can improve largely the robustness of the kernel function and therefore improve the precision of orbit determination.     

铁路缓和曲线的等间隔放样方法

介绍了铁路缓和曲线设置的目的、作用、基本原则和重要意义，阐述了等间隔放样缓和曲线的基本原理，给出了等间隔放样缓和曲线放样要素采集的基本方法，叙述了缓和曲线等间隔放样的工作过程。     

巧用CASIO fx-4800P计算器测设线路复合曲线

应用地面点与线路中线相对关系的统一数学模型，使用CASIO fx-4800P程序型计算器编制的适合野外测设线路各种复合曲线(包括直线、缓和曲线、圆曲线的任意组合)的实用程序。     

常州市数字化测绘工程及GIS建库概述

数字化地形图和地籍图是城市规划、建设和管理及国土资源管理的重要基础资料．也是开展数字城市和信息化建设的最基础平台。通过对系统总体结构的设计和应用成果介绍，分析了目前基础地理数据生产、管理和应用的现状，介绍了基于GIS的常州市数字化地形图、地籍图测绘工程总体目标及基础地理信息系统总体设计．并概述了整个数字化测绘工程的实施过程和特点。