三斑石斑鱼(Epinephelus trimaculatus)两种芳香化酶cDNA的克隆及其表达的组织特异性

通过设计引物,从三斑石斑鱼的卵巢中克隆鉴定出性腺芳香化酶(EtP450arom a)和脑芳香化酶(EtP450arom b)的cDNA序列,EtP450arom a开放阅读框为1557bp,编码519个氨基酸残基;EtP450arom b开放阅读框为1530bp,共编码510个氨基酸残基;性腺芳香化酶(EtP450arom a)和脑芳香化酶(EtP450arom b)的氨基酸序列的同源性为63.3%,并且包含了芳香化酶经典的功能保守结构。构建了芳香化酶的系统进化树。采用半定量RT-PCR方法研究了性腺芳香化酶(EtP450arom a)和脑芳香化酶(EtP450arom b)在雌性成鱼各组织中的mRNA的表达情况。组织表达结果显示,性腺芳香化酶EtP450arom a只在性腺中特异性表达,而脑芳香化酶EtP450arom b主要在脑区和垂体中表达,表明三斑石斑鱼两种芳香化酶基因的组织特异性及功能的差异。     

Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

近60年来广西北部湾气候变化及其适应研究

区域气候变化的适应研究是区域可持续发展的重要内容。文章通过收集近60年广西北部湾沿海地区的气温、降雨量和海平面变化等数据,采用小波分析、M-K检验等方法,分析该区域的气候变化特征,结果表明:年平均气温上升趋势明显,月平均气温峰值出现在6—8月,与全球平均气温变化趋势一致;年平均降雨量变化趋势不明显,5—10月是洪季;平均气温和平均降雨量存在4~6年的短周期振荡和12~16年的长周期振荡;平均气温突变年份可能是1993年或1995年,年平均降雨量的突变节点可能是2001或2007年;2007—2015年的平均海平面均高于常年海平面,平均高出常年海平面62.44mm,海平面上升造成海岸侵蚀、风暴潮增加和湿地损失增大等严重后果;建议在生态、生产、生活和管理等方面提高人类对气候变化的适应能力。     

基于风廓线雷达观测的台风“天兔”边界层演变特征研究

垂直探测台风边界层特征对于认清台风结构具有重要意义。基于1319号台风“天兔”途经的三个边界层风廓线雷达站的观测资料,结合探空数据,本文分析了“天兔”的边界层径向、切向风特征,结果表明：1）最大切向风速出现在眼区附近,在“天兔”稳定维持为强台风级别期间,最大切向风速基本稳定在1800 m高度左右,随着登陆后强度的迅速减弱,最大切向风速减弱、最大切向风速垂直范围缩小;2）最大风速所在高度和台风入流层顶高基本相近,大于依据理查森数或位温梯度所判断出的边界层高度,而基于信噪比（SNR）或其梯度所判断的混合层高度时常偏低;3）“天兔”登陆前边界层高度可以达到2100 m以上,在台风级别及以上时,各站观测到的边界层高度变化不大,基本在1200～1600 m之间,登陆后随着台风强度的减弱,边界层高度迅速减小。     

西太平洋海域卫星测高重力异常反演与精度评估

联合T/P、ERS1/GM、ERS1/ERM、ERS2/ERM、GEOSAT/ERM、GEOSAT/GM等多源卫星测高数据,基于逆Vening-Meinesz公式和EGM2008模型,采用移去-恢复方法和快速傅里叶变换算法构建了中国西太平洋海域(0°~40°N,105°E~145°E)1'×1'重力异常模型,选取两个不同特征区域航线,将构建的重力异常模型、EGM2008重力异常模型、美国Scripps海洋研究所重力异常与船测重力数据进行比较分析。结果表明,构建的重力异常模型与船测重力总体趋势变化较为一致、与船测重力比较均方根差为4.16m Gal,总体精度与EGM2008模型重力异常、美国Scripps海洋研究所重力异常相当,反演精度和分辨率达到国际领先水平。     

A study on one-dimensional consolidation of layered structured aquitard soils in a leakage system

Analytical solutions expressed using the hydraulic head for the one-dimensional consolidation of layered structured aquitard soils in a leakage system are deduced by transforming the n-layered structured soils into n + i (i = 1,2,…, n)-layered soils with gradually changing thicknesses, therein considering the behaviors of structured aquitard soils. The solutions are given in the case of instantaneous and linear decreasing confined aquifer water levels. By comparing three different examples, the effects of the structured behaviors on the consolidation characteristics are analyzed for the instantaneous decline of the confined aquifer water level.     

Lateral dynamic response of a partially embedded pile subjected to combined loads in saturated soil

In this article, an analytical solution is proposed to investigate the lateral dynamic response of a pile which is partially embedded in saturated soil layer and subjected to combined lateral and vertical loads. The saturated soil is described by Biot’s poroelastic theory and the resistance of soil is derived by potential function method. The governing equation of the pile is solved by coupling soil resistance and continuity conditions between the pile and the soil. The dynamic impedances of the pile are then obtained through transfer matrix method. To verify the validity of the proposed procedure, the present solution is compared with available solution for an idealized case. Finally, a parametric study is performed to investigate the effects of various parameters on the stiffness and damping properties of the pile-soil system. It is found that permeability of the soil and vertical load has significant effects on the dynamic response of the pile.     

Solar-, monsoon- and Kuroshio-influenced thermocline depth and sea surface salinity in the southern Okinawa Trough during the past 17,300 years

Factors influencing millennial-scale variability in the thermocline depth (vertical mixing) and sea surface salinity (SSS) of the southern Okinawa Trough (OT) during the past 17,300 years were investigated based on foraminifer oxygen isotope records of the surface dweller Globigerinoides ruber sensu stricto and the thermocline dweller Pulleniatina obliquiloculata in the AMS ¹⁴C dated OKT-3 core. The thermocline depth is influenced by surface thermal buoyancy (heat) flux, in turn controlled by the annual mean insolation at 30°N and the strength of the East Asian winter monsoon (EAWM). Strong insolation and weak EAWM tend to increase buoyancy gain (decrease buoyancy loss), corresponding to shallow thermocline depths, and vice versa. Regional SSS is influenced by the global ice volume, the Kuroshio Current (KC), and vertical mixing. A deep thermocline coincides with a high SSS because strong vertical mixing brings more, saltier subsurface KC water to the surface, and vice versa. Local SSS (excluding the global ice volume effect) became lower in the northern OT than in the southern OT after ~9.2 ka, implying that Changjiang diluted water had stronger influence in the northern sector. SSS show no major changes during the Bølling/Allerød and Younger Dryas events, probably because the KC disturbed the North Atlantic signals. This argues against earlier interpretations of sea surface temperature records of this core. Wavelet and spectral analyses of the Δδ¹⁸O_P-G (δ¹⁸O of P. obliquiloculata minus G. ruber s.s.) and δ¹⁸O_local records display 1,540-, 1,480-, 1,050-, 860-, 640-, and 630-year periods. These are consistent with published evidence of a pervasive periodicity of 1,500 years in global climate as well as EAWM and KC signatures, and a fundamental solar periodicity of 1,000 years and intermediary derived periodicity of 700 years.     

基于VMD和WinLSP相结合的GNSS-R海平面高度估测模型

全球导航卫星系统反射（Global Navigation Satellite System-Reflectometry,GNSS-R）技术是一种新兴的监测海平面高度变化的技术。本文依据GNSS-R技术中的信噪比分析法的原理,通过分析其分离趋势项和提取振荡频率的过程,建立了新的估测模型以提高反演精度。针对传统模型存在的信号分离不佳的问题,本文提出使用变分模态分解（Variational Mode Decomposition,VMD）算法替换传统的最小二乘拟合法（Least Squares Fitting, LSF）进行趋势项分量的分离。在此基础上,本文引入基于凯塞窗函数改进的LSP（Lomb-Scargle Periodogram）频谱分析法（记为WinLSP）来减弱因频谱泄露带来的反演误差。在瑞典翁萨拉的GTGU站和美国阿拉斯加州的SC02站开展的海平面高度反演实验结果表明,本文建立的估测模型相比于传统模型具有更高的反演精度。基于VMD+WinLSP估测模型得到的GTGU站反演结果的均方根误差（RMSE）、相关系数和反演点数分别为4.70 cm、0.98和5 647。与传统的LSF+LSP估测模型相比,反演精度和GNSS数据利用率分别提高了约29.7%和15.0%。SC02站的RMSE、相关系数和反演点数分别为14.34 cm、0.99和1 785,反演精度和GNSS数据利用率分别提高了约12.3%和9.4%。     

日本海中尺度涡旋时空变化特征研究

本文利用1993–2019年基于海表面高度异常的涡旋数据集和高度计数据统计分析了日本海区域中尺度涡旋的大小、极性、生命周期、振幅、传播等表面特征的时空变化规律。27年间,共探测到1 429个涡旋,气旋和反气旋数量基本相当,其中气旋675个,反气旋754个。两种极性涡旋均具有较强的季节变化：秋季较多,冬季次之,春季最少。郁陵盆地、大和盆地等为涡旋多发区域呈现西南–东北向带状分布。其中,南部海域反气旋占优,靠近津轻海峡的北部海域气旋占优。西部和南部受东韩暖流和对马暖流的驱动,涡旋移动方向与流场基本一致,北部涡旋与黎曼寒流以及副极地锋流有关。研究表明,动力学不稳定是涡旋在秋冬季大量产生的重要原因。此外,半封闭盆地、局地流场以及复杂的海气相互作用等都可能会对涡旋的产生和消亡造成一定影响。