钝顶螺旋藻部分原生质体及单细胞的制备与培养

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。     

The evolution of the coral reefs in the middle and north parts of the South China Sea and its relation with tectonic and eustatic movements

In this paper, the morphogenesis, stratigraphic sequences and dates of the coral reefs in the middle and north parts of the South China Sea are discussed, the position of the distributary regions of Cenozoic coral reefs in plate tectonics, the relationships of coral-reef evolutionary characteristics and dates with sea-basin spreading. Neogene sea-water transgression and Quaternary global climate-eustatic fluctuation are expounded and proved, and the latitudinal variation of the distribution of coral reefs in various geologic times are summed up.     

A study on remote sensing models of sea ice thickness by microwave radiometry

Extrapolating from the propagation theories of electromagnetic waves in a layered medium, a three-layer medium model is deduced in this paper by using microwave radiometric remote sensing technology which is suitable to first-year sea ice condition of the northern part of China seas. Comparison with in situ data indicates that for microwave wavelength of 10 cm, the coherent model gives a quite good fit result for the thickness of sea ice less than 20 cm, and the incoherent model also works well for thickness within 20 to 40 cm. Based on three theoretical models, the inversion soft ware from microwave remote sensing data for calculating the thickness of sea ice can be set up. The relative complex dielectrical constants of different types of sea ice in the Liaodong Gulf calculated by using these theoretical models and measurement data are given in this paper. The extent of their values is (0. 5-4. 0)-j(0. 07~0. 19).     

Study on fishery biology features of spawning group of Pseudopleuronectes yokamae(Günther) in the Sheath Bay

Pseudopleuronectes yokamae (Günther) is one of the most important economic fish species in the genus living specially in the northwest Pacific, and is distributed in the southern area of the far east sea of Russia, Japan, Korea, the Huanghai Sea, the Bohai Sea and the northern area of the East China Sea. Usually they live in nearshore waters of these areas as geographic subpopulations. P. yokamae in the Sheath Bay belongs to a local group of the Huanghai-Bohai Sea subpopulation and is distributed in the mouth and adjacent waters of the bay all the year round, and the catch of its spawning group has important economic value in the area. In this paper the fishery biology features of spawning group of P. yokamae in the Sheath Bay is systematically studied to give biology evidence for proper utilization and the multiplification of its resources.     

口服免疫药物后中国对虾某些血淋巴因子的测定及方法研究

于１９９１年４－９月，以海捕中国对虾亲虾及养殖中国对虾为材料，运用单向免疫扩散（ＳＲＩＤ）方法测定对虾血淋巴免疫因子，并采用经改进后的Ｈｏｒｏｗｉｔｚ等人的方法测定对虾体内的酚氧化酶（ＰＯ）活性，目的在于运用该两种方法对口服不同免疫药物的养殖对虾的血淋巴免疫因子及ＰＯ活力进行测定。对亲虾和养殖对虾口服不同免疫药物前后的测定结果表明，几种情况下的对虾血淋巴中均存在类似ＩｇＭ的因子，血细胞中存在酚氧化     

烟单14号夏玉米产量三因素最佳取值范围的探讨

作者根据大量试验和调查数据的分析,提出烟单14号夏玉米在高产条件下,亩穗数、穗粒数、千粒重三因素的最佳取值范围。 亩穗数:3569～4980 穗/亩; 穗粒数:423～526 粒/穗; 千粒重:259～278 克/1000粒。     

珠江河口湾伶仃洋的地形

河口湾是河流终止于大陆前缘的半围圈的海域,河水和海水分别从河口湾的两端注入并混合为冲淡水。河口湾通常是由于冰后期海面上涨被海水淹没的河流下游部分。伶仃洋就是珠江的河口湾,为华南最大的河口湾。     

Feeding habit and seasonal variation of stomach contents of sand dabs, Pseudopleuronectes yokohamae, in the South Bohai Sea

Sand dabs, an important commercial flatfish in the Bohai Sea, has recently decreased gradually in biomass, resulting from overfishing and marine pollution. Artificial culture and multiplication of sand dabs are essential and practical in China. The present paper examines the food habitof this fish to provide detailed information for its culture and multiplication.     

夏季东海西部表层海水中的pCO2及海-气界面通量

根据2001年夏季长江口及东海西部海域表层海水pCO     

Effects of light, temperature and nutrients on photosynthesis of Biddulphia regia

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