影响萼花臂尾轮虫种群增长的生态学因子的研究：温度和饵料密度的影响

本文研究了在5个温度梯度和4个饵料密度的20个试验组合下，萼化臂尾轮虫（Brachionuscalyciflorus）的种群增长规律。结果表明，该轮虫实验种群的最适温度范围是28—32℃，临界高温是36－40℃；最适饵料密度是45－65×106个／ml。这两个生态因子的最佳组合是温度32℃，饵料密度为65×106个／ml，此时，种群达最大密度3．4×103个／ml。     

一个湍能封闭模型对风暴潮海底底应力的应用研究

将一个三维湍能封闭模型应用于开阔海区的风暴潮，通过数值计算探讨了Ｔａｙｌｏｒ底摩擦二次率的拖曳系数随空间的分布及拖曳系数与水深、海底粗糙度、风向和风速等因素的关系。本文对底摩擦二次率的可靠性做了评价。     

A new type of Shipboard Meteorological Satellite Receiving-processing System

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…     

南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究

从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用.     

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

大生活用海水生化处理技术研究进展

采用海水作为大生活用水最结解沿海城市淡水资源紧缺局面的有效途径之一。该技术得以推广应用的技术关键是大生活用海水的后处理技术，文章综述了大生活用海水的生化处理技术的研究现状，并提出了今后的研究方向。     

Studies on Genus Cryptomonas from China seas

ＩＮＴＲＯＤＵＣＴＩＯＮＣｒｙｐｔｏｍｏｎａｓｉｓｕｎｉｃｅｌｌｕｌａｒｂｉｆｌａｇｅｌｌａｔｅｗｈｉｃｈｉｓａｃｏｍｍｏｎｇｅｎｕｓｏｆＣｒｙｐｔｏｐｈｙｔａ .Ｔｈｅｃｅｌｌｓａｒｅｕｓｕａｌｌｙｓｔｒｏｎｇｌｙｃｏｍｐｒｅｓｓｅｄｌａｔｅｒａｌｌｙ .ＴｈｅｒｅａｒｅｏｎｅｏｒｔｗｏｌａｒｇｅＣｈｌｏｒｏｐｌａｓｔｓｔｈａｔｃｏｎｔａｉｎｃｈｌｏｒｏｐｈｙｌｌａ ,ｃ ,ｓｏｍｅｃｈａｒａｃｔｅｒｉｓｔｉｃｃａｒｏｔｅｎｏｉｄｓａｎｄｐｈｙｃｏｂｉｌｉｎｐｉｇｍｅｎｔｓ (Ｈｕｅｔａｌ.,1 997;Ｗｅｉ…     

鱿鱼精核蛋白提取物的保健功能研究

以鱿鱼精巢组织中提取的核蛋白为主要成分制成精核蛋白提取物 ,利用功能学评价实验方法对提取物进行抗疲劳、抗氧化以及免疫调节实验。结果表明 :( 1 )该提取物能明显延长小鼠爬杆和负重游泳时间 ,减少小鼠运动时肝糖元的消耗 ,降低运动后血乳酸的水平 ;( 2 )该提取物能降低血中过氧化脂质降解产物丙二醛 ( MDA)含量、增强血中超氧化物歧化酶( SOD)活力 ;提高小鼠 NK细胞活性、巨噬细胞吞噬百分比和吞噬指数及细胞免疫水平。因而 ,该提取物有抗疲劳、抗氧化以及免疫调节作用     

碳水化合物的组合合成

碳水化合物的组合合成是一项新兴技术，该技术可以在短时间内合成大量用于进行生物活性筛选的寡糖及拟糖物。液相与固相合成技术可以极大地加快药物研究与开发进程。作者针对该技术在碳水化合物合成方面的研究进展情况进行讨论。     

黄海区水母类的生态研究

本文根据１９８２年４月２１日～５月１２日，７月２３日～８月５日，１０月１２～３０日在３４°３０′Ｎ以北．１２４°Ｅ以西黄海水域污染现状综合调查中获得的样品和资料，分析了本海区水母类的种类组成，数量分布以及季节变化。调查海区共鉴定有３４种水母，其中优势种有盘形美螅水母Ｃｌｙｔｉａｄｉｓｃｏｉｄａ，四枝管水母Ｐｒｏｂｏｓｃｉｄａｃｔｙｌａｆｌａｖｉｃｉｒｒａｔａ，五角水母Ｍｕｇｇｉａｅａａｔｔａｎｔｉｃａ。秋季（１０月）为水母类总个体数的高峰期（８．６７个／ｍ３）。水母类的分布与水温、盐度密切相关，海流或水团也起重要作用．水母类分布的变更可以反映出沿岸水和外海水相互推移和消长状况。