合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

Competition within the marine microalgae over the polar dark period in the Greenland Sea of high Arctic

Abstract-With the onset of winter, polar marine microalgae would have faced total darkness for aperiod of up to 6 months. A natural autumn community of Arctic sea ice microalgae was collected fordark survival experiments from the Greenland Sea during the ARKTIS-XI/2 Expedition of RV Po-larstern in October 1995. After a dark period of 161 days, species dominance in the algal assemblagehave changed from initially pennate diatoms to small phytoflagellates (<20μm). Over the entire darkperiod, the mean algal growth rate was-0.01 d~(-1). Nearly all diatom species had negative growthrates, while phytoflagellate abundance increased. Resting spore formation during the dark period was ob-served in less than 4.5% of all cells and only for dinoflagellates and the diatom Chaetoceros spp. We as-sume that facultative heterotrophy and energy storage are the main processes enabling survival during thedark Arctic winter. After an increase in light intensity, microalgal cells reacted with fast growth withindays. Phytoffa     

远区台风“三巴”对长江口波浪动力场的作用机制

针对远区台风对河口波浪动力场的影响问题,利用第三代波浪模式SWAN计算了远区台风"三巴"期间长江口波浪动力场分布,分析了陆架至河口区的波浪能量耗散和波致泥沙侵蚀的时空分布,发现波浪由外海向近岸传播过程中,波-波相互作用导致能量由高频向低频转换,周期和波长逐渐增大,近底层轨道流速增大,能量密度增高;阐明白帽破碎是维持深水区波浪能量平衡和限制波高成长的主要机制,底摩擦耗能和水深诱导的破碎耗能是长江口横沙东滩和崇明东滩邻近海域波高衰减的主要原因;提出波浪产生的底部切应力与相对水深有关,当波浪传播到浅水区时,波长和周期越大,波浪切应力越大。研究揭示了与河口相距数百公里的远区台风能够对长江口波浪动力场产生明显影响,河口水下三角洲前缘是最容易受到波浪侵蚀的区域,研究成果弥补了目前关于陆架远区台风对河口波浪动力场影响研究的不足,对深化认识远区台风对长江口动力环境、地貌演变、航运安全和滩涂保护等有重要科学意义。     

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.     

THE CLIMATIC CHANGES AND THE COASTAL ENVIRONMENT

Although the Yellow River Delta and Bohai Sea coastal zone are long distance and different environment with Po River Delta and Adriatic Sea coastal zone, the comparison of data of two different areas and the evolution trend of two areas are quite similar. The influence of natural climatic changes on the evolution of the deltas and coastal zones exists both in ancient and modern times. The cold/wet period occurred in 1300—1400A. D. and 1550 —1850 A.D. within this"large scale" climatie cycle, and shorter periods (10— 35 years) of cold rainy weather alternated with warm/dry period are known as "Bruckner cycles" which have influence on the evolution of the deltas and coastal zones.     

大黄鱼病原弧菌拮抗放线菌的筛选与人工诱变

从厦门集美海滩底泥分离得到38株放线菌，用琼脂块法测定它们对大黄鱼病原弧菌-溶藻弧菌和副溶血弧菌的拮抗效果，选择两株拮抗效果较好的放线菌进行诱变，测定诱变后各菌株的抗菌效果，并选择抗菌效果较好的菌株进行第二次诱变，如此反复诱变3次，共得到97株放线菌，结果表明；38株放线菌中的22株对两株病原菌有一定的拮抗作用；用紫外线照射可以获得少量对两株病原菌拮抗作用加强的菌株。     

0010号台风"碧利斯"对厦门港湾表层海水中溶解无机砷含量的影响

本文研究了0010号台风“碧利斯”影响期间及其随后的恢复期中,厦门港湾中九龙江口和西港海域站表层海水总溶解砷（TDIAs)、As^3 含量和As^3 /As^5 值的变化特征。结果表明,观测期间两站TDIAs和As^5 含量均比非台风时期呈数倍增加,经10d后仍未恢复到非台风时的水平。As^3 含量也有较大幅度的增加,但波动较大。文中还对观测期间影响溶解无机砷含量的因素进行了初步的探讨。     

应用数字地形模型方法分析漳州港海底冲淤变化

利用漳州港海域无围堰填海造地前后的水深测量数据，结合海域地质钻探、表层和柱状沉积物资料，用数字地形模型方法计算了该区海底的冲淤量，分析了冲淤变化及其影响因素，结果显示：（1）淤积主要发生在近岸海域，其主要原因是近岸3-10m厚的海底淤泥层在回填土的挤压和推移下发生形变，产生淤浅假象；另一个原因是回填时部分泥沙流失入海，淤积在近岸海域，致使淤积强度增大。（2）由近岸往外，淤积强度逐渐减弱，并过渡为产中刷状态。     

光诱鱿鱼浮拖网渔具渔法试验报告

1989－1999年开展光诱鱿鱼浮拖网渔具渔法研究的生产试验结果为：1998年生产试验86个夜（航次），总产量33．225t，其中鱿鱼产量为12．470t，占总产量的37．5％，总产值139000元，比同村光诱敷网的平均产量（31．62t）高5．1％左右。1999年生产试验80夜（航次），总产量54．66t，其中鱿鱼产量31．92t，占总产量的58．4％，总产值187000元。试验网产量比同村光诱敷网的平均产量（45．64t)增产19．76％，产值增加22．65％，鱿鱼产量占总渔获量的比例提高近10个百分点，投入产出比为1：1．684。取得了较好的试验结果，达到了预期试验目的。此外，文中还讨论了水上灯数量，网具规格和柔性网板等对生产效果和捕捞对象的影响及适应性情况，并建议开展光场强度及其分布的海上实测和光场强度对鱿鱼，趋光性鱼类的行为习性以及生长影响等试验研究，制定出光诱鱿鱼浮报网和敷网作业诱鱼灯数量和分布标准，以合理利用鱿鱼和中上层鱼类资源，实现可持续发展。     

AN ECOLOGICAL STUDY OF FOULING ORGANISMS IN XISHA ISLANDS

Monthly and seasonal panel experiments were carried out in the Yongxing Island from September 1979 to August 1981. Altogether 119 species of fouling organisms are collected, of which, Hydroides elegans, Serpula vermicular is, Scrupocellaria longispinosa, Amathia distant, Enteromorpha spp. and Ostrea spp. are dominant species. Fouling of organisms may occur all the year round with evident seasonal variations, Species succession in the process of community formation is very evident and the climax has arrived after two years. Great variation exists between fouling organisms in different years and at different stations.