基于R树的分布式并行空间索引机制研究

为提高分布式并行计算环境下海量空间数据管理与并行化处理的效率,基于并行空间索引机制的研究,设计一种多层并行R树空间索引结构。该索引结构以高效率的并行空间数据划分策略为基础,以经典的并行计算方法论为依据,使其结构设计在保证能够获得较好的负载平衡性能的前提下,更适合于海量空间数据的并行化处理。以空间范围查询并行处理的系统响应时间为性能评估指标,通过实验证明并行空间索引结构具有设计合理、性能高效的特点。     

地图数据缩编更新的模式分类与选择

地图数据缩编更新模式是缩编更新技术流程设计及软件模块开发的基础。根据缩编和更新对象的不同归纳了缩编更新的4种模式,结合当前地图自动综合理论技术水平与数据组织管理现状,认为现阶段最合理的模式为新旧数据叠加缩编更新模式。基于该模式分析道路数据缩编更新的技术流程并开发了相应的软件模块,应用于国家1∶5万地理信息系统中道路数据的更新,从而验证了该模式的可行性。     

Evaluation of the four potential Cretaceous-Paleogene (K-Pg) boundaries in the Nanxiong Basin based on evidences from volcanic activity and paleoclimatic evolution

Determining the location of the Cretaceous-Paleogene(K-Pg) boundary in terrestrial strata is highly significant for studying the evolution of terrestrial ecosystems at the end of the Cretaceous(especially the extinction of non-avian dinosaurs). At present, research on terrestrial K-Pg boundaries worldwide is concentrated in the middle and high latitudes, such as North America and Northeast China. Although many studies have also been carried out in the Nanxiong Basin, located at low latitudes(which has become the standard for dividing and comparing the continental K-Pg stratigraphy in China), many researchers have proposed four possible boundaries from different perspectives. Therefore, the exact location remains to be determined. In this study, the total mercury(Hg) content, environmental magnetism, geochemistry, and other parameters for the samples collected near the four boundaries were determined and compared with existing records. Results indicated that: 1) The total Hg content significantly increased in the upper part of the Zhenshui Formation and Pingling part of the Shanghu Formation with sharp fluctuations. As per latest dating results of Deccan Traps, the significantly high Hg value was attributed to the Deccan Traps eruption. Boundary 1 was located in the middle of the Hg anomaly interval, which was consistent with the relationship between the global K-Pg boundary and time of volcanic eruption. 2) The reconstructed paleoclimate evolution curve revealed that the red sediments in the basin recorded the late Maastrichtian warming event(66.2 Ma). Regarding the relationship between the four boundaries and this warming event, only boundary 1 was found to be closest to the real K-Pg boundary of the Nanxiong Basin.     

斜带髭鲷(Hapalogenys nitens)血细胞发生及外周血细胞化学染色观察

通过Wright-Giemsa染色对斜带髭鲷[Hapalogenys nitens (Richardson)]的头肾、体肾、脾脏、肝脏四组织涂片、印片的观察,发现其头肾、体肾、脾脏是其主要的造血器官。头肾能发育生成各类型血细胞,体肾能生成红细胞和粒细胞,脾脏能生成淋巴细胞和红细胞。肝脏中未发现幼稚型血细胞。血细胞发生大致经过原始、幼稚和成熟三个阶段。细胞化学染色显示:外周血细胞中,除淋巴细胞外,均有糖原;嗜中性粒细胞含有ACP、ALP、NACE、αNAE和POX;嗜酸性粒细胞含有ACP、NACE和POX;单核细胞含有ACP、αNAE和POX;小淋巴细胞有ACP。研究发现斜带髭鲷是一种抗病能力较强的鱼类。血细胞组织化学染色可作为斜带髭鲷疾病诊断的辅助手段。     

中国南海北部珠江口-琼东南盆地钻井岩屑地球化学特征及深水区天然气聚集特征

The Qiongdongnan Basin and Zhujiang River(Pearl River) Mouth Basin, important petroliferous basins in the northern South China Sea, contain abundant oil and gas resource. In this study, on basis of discussing impact of oil-base mud on TOC content and Rock-Eval parameters of cutting shale samples, the authors did comprehensive analysis of source rock quality, thermal evolution and control effect of source rock in gas accumulation of the Qiongdongnan and the Zhujiang River Mouth Basins. The contrast analysis of TOC contents and Rock-Eval parameters before and after extraction for cutting shale samples indicates that except for a weaker impact on Rock-Eval parameter S_2, oil-base mud has certain impact on Rock-Eval S_1, Tmax and TOC contents. When concerning oil-base mud influence on source rock geochemistry parameters, the shales in the Yacheng/Enping,Lingshui/Zhuhai and Sanya/Zhuhai Formations have mainly Type Ⅱ and Ⅲ organic matter with better gas potential and oil potential. The thermal evolution analysis suggests that the depth interval of the oil window is between 3 000 m and 5 000 m. Source rocks in the deepwater area have generated abundant gas mainly due to the late stage of the oil window and the high-supper mature stage. Gas reservoir formation condition analysis made clear that the source rock is the primary factor and fault is a necessary condition for gas accumulation. Spatial coupling of source, fault and reservoir is essential for gas accumulation and the inside of hydrocarbon-generating sag is future potential gas exploration area.     

苏北浅滩海底地下水排放及其对海域营养盐通量的贡献

海底地下水排放(SGD)是近海海域的一个重要的营养盐来源。本研究借助多种天然镭同位素对春季苏北浅滩海域的SGD及其携带入海的营养盐通量进行量化评估。研究发现:苏北浅滩海域的~(224)Ra、~(223)Ra和~(226)Ra等镭同位素的浓度水平较高,呈现近岸高、远岸低的分布趋势;根据~(224)Ra/~(226)Ra的"表观年龄模型"估算的水龄的分布情况推断,春季该海域表层水体主体流向为东北向,流速约为0.1m/s,这与前人物理海洋数值模拟结果一致;最终利用226Ra质量平衡模型发现海域的SGD通量为(46±29)cm/d,由其携带入海的溶解态无机氮、磷、硅营养盐(DIN、 DIP、 DSi)等的通量分别为(2.6±3.1)×1~09、(3.0±2.5)×10~6和(5.5±4.2)×10~8mol/d。     

基于经验模态分解和小波分解估算海气通量涡相关计算中的截断时间尺度

基于时长38天的海表风场实测数据,应用经验模态分解(EmpiricalModeDecomposition,EMD)和小波分解(Wavelet Decomposition, WD)这两种数据处理方法首先对涡相关法中的截断时间尺度(CutoffTimescale,CTS)进行估算,结果显示:基于EMD与WD方法估算出的CTS一般都在40秒左右(EMD的结果略小),远远小于传统涡相关法中CTS的取值(固定为10分钟),且EMD和WD的使用使得每一段数据都能够根据自身的湍流特点而获得合适的CTS; EMD方法和WD方法有效的去除了计算结果中的非湍部分,且对通量传输方向的刻画也更加合理,极大提高了通量的计算精度,所得通量与传统方法计算的通量偏差平均值高达45%;研究还对EMD和WD的优缺点进行了对比分析,结果表明EMD相比于WD有更高的自主性,而WD对信号的分离程度则更高。     

太平洋深海沉积物来源的菌株Tenacibaculum sp.HMG1对孔雀石绿降解特性的研究

本文在太平洋深海沉积物中分离得到一株孔雀石绿降解菌株,鉴定命名为Tenacibaculum sp.HMG1。通过菌株生长实验和高效液相色谱的研究表明, HMG1菌株可以在20 mg/L的孔雀石绿中维持较快的生长速率,并且在12 h内可降解98.8%的孔雀石绿,这证明该菌株具有很高的孔雀石绿耐受能力和降解活性。通过基因组测序在HMG1菌株发现一条过氧化物酶基因可能参与了孔雀石绿的降解,随后利用原核表达获得了相应的重组蛋白。实验表明,该重组过氧化物酶具有极强的活性,可在1000 mg/L的孔雀石绿中发挥降解功能。本文利用液相色谱-质谱联用（LC-MS）技术对孔雀石绿的菌株降解产物和重组酶降解产物进行鉴定,并基于鉴定结果推测了两种降解途径。结果发现两种降解方式存在共同的降解途径。此外,孔雀石绿降解条件的实验结果证明重组过氧化物酶可以在低温（20℃）、复杂的pH值（6.0–9.0）、高盐度（100 mmol/L）、金属离子和EDTA等反应条件下依旧维持很高的孔雀石绿降解活性。以上实验结果表明,HMG1菌株和重组过氧化物酶均在孔雀石绿污染生物修复方面具有很大潜力。     

Establishment and application of an intelligent treating method for oil spill identification

In the identifying process of an oil spill accident, manual integral and artificial visual comparison are commonly used at present to determine the oil spill sources, these methods are time-consuming and easily affected by human factors. Therefore, it is difficult to achieve the purpose of rapid identification of an oil spill accident. In this paper, an intelligent method of automatic recognition, integration and calculation of diagnostic ratio of Gas Chromatography-Mass Spectrometer (GC/MS) spectrum are established. Firstly, four hundreds of samples collected around the world were analyzed using a standard method and Retention time locking technology (RTL) was applied to reduce the change of retention time of GC/MS spectrum. Secondly, the automatic identification, integration of n-alkanes, biomarker compounds, polycyclic aromatic hydrocarbons and calculation of the diagnostic ratios were realized by MATLAB software. Finally, a database of oil fingerprints were established and applied successfully in a spill oil accident. Based on the new method and database, we could acquire the diagnostic ratios of an oil sample and find out the suspected oil within a few minutes. This method and database can improve the efficiency in spilled oil identification.     

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.