近42年雅鲁藏布江中游四季气温变化特征分析

利用 1957～ 1998年雅鲁藏布江中游拉萨、日喀则、泽当、江孜 4个站各月平均气温资料 ,通过线性趋势估计和多项式函数拟合分析了该流域春、夏、秋、冬 4季气温长期趋势变化和周期变化 ,并利用滑动T检验等方法讨论了气温突变的问题。结果表明 :在过去 4 2年里 ,雅鲁藏布江中游地区各季气温均有明显的上升趋势 ,其中冬春季增温显著 ,80年代初沿江四季气温除秋季外都出现了明显的增温突变     

基于兰利法的泥石流临界雨量实验研究

分析了目前泥石流临界雨量确定常用方法存在的问题 ,以及泥石流临界雨量实验研究中实验降雨量确定的随意性和盲目性 ,提出以兰利 ( Langlie)法为指导进行实验 ,使实验降雨量的确定有了理论依据 ,减少了取得可靠实验结果所需实验次数 ,从而使泥石流临界雨量实验研究更趋完善和可行     

广西第三代1∶50万数字地质图特点及其意义

文章扼要介绍了广西第三代 1∶5 0万数字地质图的主要内容、特点及编图新理论和新技术 ,并指出该项成果所具有的科学意义和实用价值。     

西安地裂缝场地勘察及受损建筑物的治理

结合地裂缝成因、性质及地质环境,提出了西安地裂缝场地勘察行之有效的方法,对拟建场地进行地裂缝监测,可预报地裂灾害。根据地裂缝对建筑物破坏的特点,结合地基处理技术提出了对受地裂缝影响建筑物治理的方法,对因土地紧张而需在地裂缝影响范围内建造的建筑物,从设计上考虑预纠偏方案作为预防措施     

植物生长调节剂在龙眼短枝扦插中的应用

用３种生长素对龙眼插条进行处理,结果表明：龙眼插条不定根由愈伤组织分化而成,ＩＢＡ 能促进插条的愈伤组织发生,２．４－Ｄ 对插条叶片的保持效果最好,龙眼扦插的难点在于生根过程的后期阶段     

Mechanism on bipolar distribution of Permian brachiopods

By reasearch on geographic distribution, nine genera in bipolar distribution are selected from Permian brachiopods. These taxa originated from middle-high latitude areas in the boreal realm, of which five genera were derived from Late Carboniferous, and other four genera originated from Permian. They were all in bipolar distribution during some different stages in Permian. Specific diversity for each genus was high in the boreal realm, whereas in the Gondwana realm was very low. Perdurability was long in the boreal realm, and short in the Gondwana realm. It was the time when these nine genera came to their maximum diversity that these genera appeared in the Gondwana and formed bipolar distribution; while they also migrated to the low latitude from high latitude. This shows very close relationship between several main cooling events in Permian and the migration of genera from the boreal realm to the Gondwana realm through the Tethys. Therefore, the cooling events might be the main drive which caused these cold-water-type brachiopods migrated to the Gondwana realm and being bipolar distribution. In this process, the planula tolerance to warm water would be another important factor.     

机械光栅式测缝计应用于无砟轨道板上拱监测的可行性分析

高速铁路轨道的稳定性和平顺性是高速铁路正常运营的关键,因此对运营期高速铁路的轨下结构持续地开展变形监测是十分必要的。当前我国对轨道板变形的检测主要是依靠人工肉眼观察式的现场检查和常规水准测量方式进行监测,效率低下,难以在有限的天窗时间内完成辖区内轨道结构的全覆盖检测。基于此,从理论分析和实验测试两方面探讨机械光栅式测缝计应用于无砟轨道板上拱自动化监测的可行性,结果表明机械光栅式测缝计能够抵抗高温、淋水和振动等不良条件的影响,满足轨道板上拱监测的精度要求。     

基于红外探测器焦平面成像的白天测星技术研究

对于大气层以下的传统可见光天文导航,存在白天日照强烈而无法观测星体,数据缺乏连续性,可观测恒星数量少,无法实现全天时天文导航等问题。文中设计并探究利用红外探测器焦平面成像技术来取代可见光CCD成像技术的短波红外天文导航方法,分析短波红外波段相对于可见光波段,在大气层内白天观星实现全天时天文导航的优势,并对其可行性进行实验分析。实验结果表明,基于红外探测器的天文导航方法,在太阳辐射较弱的清晨或黄昏等时段,可以成功拍摄到可见光CCD仪器无法观测到的短波红外波段的恒星,对于实现全天时天文导航具有一定的参考意义。夜间短波红外波段的可供观测恒星数量远远多于可见光恒星数量,这对于增加多余观测量,保证连续测量输出,提高天文导航定位定向的精度,具有实际的应用价值。     

油罐区砖砌防火堤的抗震验算

介绍了油罐区砖砌防火堤的抗震验算基本原理和方法,并按照验算方法实现其通用化计算。防火堤主要受到4种荷载作用,即自重惯性力及其引起的弯矩、堤内静液压力及其引起的弯矩、堤内动液压力及其引起的弯矩、自重引起的垂直力。按不同烈度要求计算得到4种荷载作用校核堤的截面抗力和地基强度。通过两个算例检验该方法在抗震鉴定中的应用。两个防火堤典型断面的截面强度、抗滑动及抗倾覆稳定性、地基强度验算结果分别符合抗震设防7度和8度要求。防火堤作为备用体系,其抗震验算程序的通用性具有实用意义。     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.