Detecting and attributing vegetation changes in Taihang Mountain,China

Attributing vegetation changes provide fundamental information for ecosystem management,especially in mountainous areas which has vulnerable ecosystems. Based on the Normalized Difference Vegetation Index(NDVI) data, the spatial-temporal change of vegetation was detected in Taihang Mountain(THM) from 2000 to 2014. The topographical factors were introduced to interpret the response of vegetation variation to climate change and human activities. Results showed that the avegaged NDVI during growing season showed a single-peak curve distribution, with the largest value(0.628) among 1600-1800 m. A significant greening trend was detected in THM, with the largest increasing rate(0.0078 yr~(-1)) among the elevation of1600-1800 m and slope gradient between 3~5°. The partial correlation and multiple correlation analyses indicated that vegetation variation in more than81.8% pixels of the THM was mainly impacted by human activities. In the low elevation zones less than1000 m, increasing precipitation is the principle factor promoting vegetation restoration, whereas in the high elevation zones of THM, temperature is the restricted factors impacting vegetation variation.Considering the dramatic climate change in the future,further studies should be conducted to explore inherent mechanism of vegetation growth to dynamic environment changes.     

Relationships between body weight and other morphological traits in young sea cucumbers Apostichopus japonicas

Correlation, multiple regression, and path analyses were used to investigate the relationships between body weight and three other morphological traits in juvenile Japanese sea cucumbers Apostichopus japonicus . We measured live body weight (BW), body length (BL), numbers of papillae (NP), and numbers of tube feet (NF) at 60, 80, 100, and 130 days post-hatching (dph). We calculated path correlation coefficients, correlation indices ( R 2 ), and coefficients of determination with BW as the dependent variable and the other morphological traits as independent variables. The coefficient of variation for BW was high across all age groups, and all measured morphological traits were significantly correlated ( P < 0.01). BL had the greatest direct eff ect on BW across all age groups (60 dph, 0.526;80 dph, 0.404;100 dph, 0.620;and 130 dph, 0.681), while NF had the greatest indirect eff ect on BW across all age groups (60 dph, 0.528;80 dph, 0.452;100 dph, 0.666;and 130 dph, 0.603). Regression analyses between morphological traits and BW indicated that R 2 was greater than 0.85 only in the 100-dph specimens. The indirect eff ects of the other measured morphological traits on BW were age-dependent. The optimal regression equations, as determined with stepwise regression, were, for 60-dph specimens: BW 60 =10 (-3.04+0.092BL+0.014NP+0.014NF)( R 2 =0.632);for 80-dph specimens: BW 80 =10 (- 3.035+0.056BL+0.017NP+0.02NF)( R 2 =0.686);for 100-dph specimens: BW 100 =10 (- 3.742+0.069BL+0. 633 · lg (NP)+0. 464 · lg (NF))( R 2 =0.893);and for 130-dph specimens: BW 130 =10 (- 2.472+0.065BL+0.012NP)( R 2 =0.774). Our work clarified the correlation between various morphological traits and body weight of a commercially-important sea cucumber species ( A . japonicus ). Our predictive models for body weight might be useful for the aquaculture and selective breeding of A . japonicus . These models might also provide theoretical support for the indirect selection of traits that are difficult to select directly.     

漠阳江入海口海岸线分维及其机制分析

分维数是反映海岸线复杂程度的客观依据。结合国内外海岸线分形研究进展,本文以漠阳江入海口附近海域为研究区,基于Landsat 8 OLI遥感影像对研究区域提取瞬时水边线,并利用潮汐数据进行校正得到海岸线;利用Fractal Fox2.0计算获得该海岸线的分维数为1.071;通过对研究区的分形机制分析,认为漠阳江附近的断裂构造对海岸起宏观控制作用,从而对海岸线的分维值也起了决定作用。根据断裂体系模型计算得到西侧海岸线的理论分维值为1.089,而对数螺线模型计算得到东侧海岸线的分维值为1.171;实际分维值相对理论值偏小,说明海岸带的沉积与侵蚀影响了分维值。此外,根据本区海岸侵蚀原因的分析,对分维机制进行了探究,初步论证的分维值大小可为海岸的冲淤变化研究提供参考。     

海上孤立混凝土构筑物的爆破拆除

在海洋工程中,采用爆破手段拆除海上孤立混凝土构筑物或清理孤立大块石是一种快速、有效的方法。文中详细介绍了爆破方案的确定、爆破孔网参数的设计、爆破药量的计算以及安全防护措施,对于类似工程的实施具有重要的参考价值。     

6种海洋致病性弧菌36kDa外膜蛋白特性分析

研究本着筛选和制备弧菌共同外膜蛋白亚单位疫苗,用十二烷基肌氨酸钠抽提并结合超速离心的方法提取了鳗弧菌(Vibrio anguillarum)、鱼肠道弧菌(V.ichthyoenteri)、溶藻弧菌(V.alginolyticus)、副溶血弧菌(V.parahaemolyticus)、哈维氏弧菌(V.harveyi)和创伤弧菌(V.vulnificus)6种海洋致病性弧菌的主要外膜蛋白, SDS-PAGE图谱比较发现这6种弧菌都存在36 kDa的外膜蛋白条带.通过电泳洗脱分离纯化这6种弧菌的36 kDa外膜蛋白,并制备了鳗弧菌菌株SJ060621的36 kDa外膜蛋白多抗.ELISA和Western-blot印迹结果显示,仅鳗弧菌SJ060621、S010610-1和哈维氏弧菌的36 kDa外膜蛋白存在免疫交叉反应;双向电泳分析了鳗弧菌SJ060621、S010610-1、哈维氏弧菌和溶藻弧菌的36 kDa外膜蛋白,2-DE图谱显示出鳗弧菌SJ060621和S010610-1均由3种等电点相近的蛋白组成,存在很高的同源性,2株鳗弧菌与溶藻弧菌和哈维氏弧菌在蛋白质组成数量和等电点上都存在较大的差异,结果说明通过主要外膜蛋白的分子量和免疫特性研究,可为筛选不同弧菌共同外膜蛋白的亚单位疫苗提供有利参考.     

牙鲆淋巴囊肿病毒靶器官上病毒结合蛋白的鉴定

利用抗牙鲆淋巴囊肿病毒单克隆抗体的间接免疫荧光检测技术,在患淋巴囊肿病牙鲆的表皮、鳃、胃、肠及肾中发现淋巴囊肿病毒的感染,以此确定为病毒感染的主要靶器官。用NP-40细胞裂解液分别抽提靶器官的总蛋白,利用病毒铺覆蛋白印迹技术(VOPBA)对靶器官上淋巴囊肿病毒结合蛋白进行鉴定,结果显示在胃、肠及肾的组织蛋白中均有1个分子量为27.8 kDa的蛋白与淋巴囊肿病毒特异结合,在鳃和表皮的组织蛋白中分别存在分子量为37.6 kDa和56.0 kDa的蛋白与淋巴囊肿病毒特异结合。推测在淋巴囊肿病毒靶器官上的这3种病毒结合蛋白有可能是病毒的受体蛋白。     

GNSS地面测试环境中关于影响站址选择因素的考虑

卫星导航产品在投入正式运行之前需要在地面上对其进行测试和校准,因此需要在地面建立模拟天上GPS卫星星座的测试环境,并通过修建伪卫星发射塔来发射类GPS卫星信号。测试环境中关于伪卫星发射塔站址选择问题是一个值得关注的硬件建设环节,它直接影响了系统的运行性能,本文通过对五个影响站址选择的因素进行论述,详细地说明了它们各自的影响特征,给出了相应的结论。     

In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.     

海南岛西北部莲花山周缘海相地层的区内对比及意义

根据岩性、产状和地貌等特征,对海南岛西北部莲花山周缘海岸出露的前全新世海相地层5个典型剖面进行区内对比,以恢复该套地层的产出序列,并推测海相地层的分布范围。结合海相地层的沉积时代和莲花山周缘海岸的地貌特征,探讨了该海岸带的地貌演化过程:中更新世以前,有河流自东南向西北经德义岭、莲花山入海,海相地层沉积区属于滨岸环境,是该海岸带当时受海水进退作用的主要区域,接受着波浪从北面、河流从南面带来的沉积物;中更新世至今,受雷琼地区火山活动的影响,河流被隆起的火山锥阻挡而截流或改道,海相地层沉积区则被抬升成为陆地,并在全新世海岸侵蚀作用下,形成了现今宽广的海蚀平台,海岸后退平均速率约为10 cm/a。     

南海南部断裂的分形研究

分形几何作为定量描述自然界中复杂非线性现象的强有力工具,将其应用于南海南部断裂体系的研究中,通过数盒子法计算了断裂分布的分维值。研究结果表明这种方法是可行的,断裂分布的分维值能有效地描述断裂的空间分布特征。南海南部断裂体系在标度区间25～250km内具有很好的统计自相似性,全部断裂的分维值为1．6601,北东向断裂的为1．3875,北西向断裂的为1．2693。根据断裂分维等值线,沿西南海盆扩张轴,两侧断裂呈对称展布;北东向的与北西向的在空间上有一定的互补性,这反映了两组断裂在发育过程中的相互制约性。结合南海的构造演化及南海南部油气盆地的分布,初步探讨了断裂分形特征与南海的构造演化及断裂分形特征与油气盆地分布之间的关系。