Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion

1　Introduction　Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…     

平阴县土壤中重金属元素异常成因研究

通过对平阴县周边及其西部农田区采样调查,平阴县周边元素异常具有异常衬度较高,富集系数大等特征,西部农田区异常具有各元素套合关系好,多数异常衬度不高,富集系数小的特点。结果表明,西部农田区异常是以As,Cd为主要成分的异常,元素含量与SiO2,Al2O3,Fe2O3等常量组分间具有显著的相关性,随SiO2含量的增加而降低,随Al2O3,Fe2O3含量的增加而增加,说明土壤常量组分及其所决定的土壤质地是导致异常形成的主要因素;而平阴城区异常是以S,Hg,As,Zn,Mn等元素为主的异常,是典型的人为综合因素（化工烟尘、工厂污水、生活垃圾）产生的异常。     

枣庄市薛城区治理非法盗采煤炭资源行为的对策

枣庄市薛城区煤炭资源丰富,辖区内含煤面积达180km2,2000年以前4个乡镇曾有矿井70多对,目前正在生产的矿井还有16对,由于废弃矿井多,部分闭坑矿井措施不彻底、不到位,特别是陶枣矿区露头煤距地表埋藏较浅,给违法分子非法盗采留下可乘之机。非法盗采煤炭资源行为既扰乱了正常的矿业秩序,造成资源极大浪费,严重危及周边合法煤矿的安全生产,同时又对社会安全稳定带来了隐患,严厉打击非法盗采煤炭资源行为刻不容缓。     

东秦岭石界河群中石榴石成分环带所记录的PTt轨迹

位于河南省西峡县北部地区的石界河群榴云片岩中的石榴石发育有很好的结构和成分环带。本文应用微分热力学方法对其环带成分进行计算,确定出一条顺时针方向的PTt轨迹。该轨迹与应用矿物共生组合世代关系确定的PTt轨迹十分吻合。     

SEA LEVEL RISE OF THE ZHUJIANG RIVER DELTA AND NEOTECTONICS

A linear regression analysis of 28 selected tide-gauge stations of the Zhujiang Estuary shows that there has been a tendency of local sea level rise at a rate of 2.028 mm per year. The origin of the variation is significantly attributed to the local tectonic movement of discrepant fault-block. Based on this, four types of relative local sea level changes are classified. According to calculation, half of the fertile land, or 800 km2 of the delta plain will have been submerged by sea water by about 2040. This will yield a significant influence on the economic construction and human activities.     

A comparison of different Gracilariopsis lemaneiformis (Rhodophyta) parts in biochemical characteristics,protoplast formation and regeneration

Gracilariopsis lemaneiformis is a commercially exploited alga. Its filaceous thallus can be divided into three parts, holdfast, middle segment and tip. The growth and branch forming trend and agar content of these three parts were analyzed, respectively, in this study. The results showed that the tip had the highest growth rate and branched most, although it was the last part with branch forming ability. The holdfast formed branches earliest but slowly. Holdfast had the highest agar content. We also assessed the difference in protoplast formation and regeneration among three parts. The middle segment displayed the shortest enzymolysis time and the highest protoplast yield; whereas the tip had the strongest vitality of protoplasts formation. Juvenile plants were only obtained from the protoplasts generated from the tip. These results suggested that the differentiation and function of G. lemaneiformis was different.     

A novel model to assess soil productivity in the dry-hot valleys of China

Accurate evaluation of soil productivity has been a long-standing challenge. Although numerous models for productivity assessment exist, most are cumbersome to use and require substantial parameter inputs. We developed a new empirical soil productivity model based on field investigations of soil erosion, soil physicochemical properties, and crop yields in the dry-hot valleys (DHVs) in China. We found that soil pH, and organic matter and available potassium contents significantly affected crop yields under eroded conditions of the DHVs. Moreover, available potassium content was the key factor affecting soil productivity. We then modified an existing soil productivity model by adding the following parameters: contents of effective water, potassium, organic matter, and clay, soil pH, and root weighting factor. The modified soil productivity model explained 63.5% of the crop yield. We concluded that the new model was simple, realistic, and exhibited strong predictability. In addition to providing an accurate assessment of soil productivity, our model could potentially be applied as a soil module in comprehensive crop models.     

CLIMATIC TREND INDICATED BY VARIATIONS OF GLACIERS AND LAKES IN THE TIANSHAN MOUNTAINS

ＣＬＩＭＡＴＩＣＴＲＥＮＤＩＮＤＩＣＡＴＥＤＢＹＶＡＲＩＡＴＩＯＮＳＯＦＧＬＡＣＩＥＲＳＡＮＤＬＡＫＥＳＩＮＴＨＥＴＩＡＮＳＨＡＮＭＯＵＮＴＡＩＮＳ￥ＨｕＲｕｊｉ；ＹａｎｇＣｈｕａｎｄｅ；ＭａＨｏｎｇ；ＪｉａｎｇＦｅｎｇｑｉｎｇ（ＸｉｎｊｉａｎｇＩｎ...     

中国城市群热岛效应时空演变及其影响因素分析

基于长期不透水面和MODIS地表温度数据,分析2000—2015年中国城市群扩张及热岛效应时空演变,进而综合采用冗余分析(RDA)、线性回归分析和变异分配分析(VPA)等方法,揭示城市群城市热岛效应的驱动机制。结果表明：城市群内建成区面积快速扩张,不透水面比例从2000年2.08%增长到2015年5.33%,且主要集中于珠江三角洲等沿海城市群;2000—2015年,夏季热岛分布较广,且白天强度高于夜晚。东部以及大部分北部城市群如哈长城市群等,降温强度较大,但其夜晚热岛效应在不同程度增强。冬季夜晚比白天热岛分布广、强度高,北方、西北、东部等地区夜晚热岛效应也在增强;自然环境因素显著影响城市群热岛强度,降水对夏季夜晚热岛强度起显著负贡献(22%),纬度越高,冬季夜晚热岛强度也越高。人为因素显著影响夜晚热岛分布和城市群内热岛强度的平衡,城市植被覆盖显著减少夜晚城市群内热岛分布,灯光强度对夏季夜晚热岛强度起显著负贡献(24%),对热岛比例起显著正贡献(27%),人口密度对夏季夜晚热岛强度起显著负贡献(31%);自然环境因素对热岛强度的贡献占主导,而人为干扰因素对热岛比例的贡献占主导。     

从珠三角到大珠三角再到粤港澳大湾区：改革开放以来中国的国家尺度重组

尺度重组是解释当前国家、城市等地域组织参与全球化竞争的工具媒介。文章以中国华南地区改革开放以来广东、香港、澳门三地跨界合作为例,借鉴行动者网络理论的研究方法深入剖析珠三角、大珠三角及粤港澳大湾区3个“新国家空间”的尺度重组过程,揭示中国尺度重组的政治经济逻辑。研究发现,行动者拥有的资源决定了其在尺度网络中的地位,而中国的“新国家空间”形成主要依托中央政府推动,其发展目标的设定具有决定性作用,港澳特区政府、广东省地方政府、乡镇企业和外资企业等其他行动者在协同合作中将自身的发展目标与之融合形成了“强制通过点”。纵观三阶段的演变过程,制度因素的重要性越来越得到凸显。