Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

天然热释光技术在海洋油气田勘查中的应用

沉积物热释光能有效地反映海洋环境下深部油气田引起的微弱放射性异常。热释光在不同的测试粒级、不同的岩性中的丰度不同，结果为粗粒级＞细粒级、砂岩＞粉砂质砂岩＞泥质粉砂岩＞泥岩。热释光也与部分金属元素含量有关，Ti、Ba、Zn、Fe^3 /Fe^2 与热释光呈正相关关系。热释光与烃类也呈较强的正相关性。不同采用深度热释光测量结果表明，热释光剂量随沉积物的沉积深度和沉积时间的增加而增加，深层样的热释光对油气藏的指示效果更好。     

卵形鲳鲹早期卵子发生显微及超微结构的研究

本文研究了卵形鲳鲹早期卵子发生的显微与超微结构特点。研究结果表明在1～2龄鱼卵巢中卵原细胞进入首次成熟分裂前期的结构特点，3～4龄鱼才开始进入小生长期，文中讨论了此鱼性腺成熟的年龄以及如何加速性腺发育，为人工繁殖提供科学依据。     

黄渤海海域贝类麻痹性贝毒的检测与分析

采用小白鼠生物测试法和高效液相色谱法对2002~2005年我国黄渤海海域采集的贝类样品进行了麻痹性贝毒毒性检测,结果显示大连海域的虾夷扇贝含有麻痹性贝毒,有毒样品均出现在5月和6月,部分虾夷扇贝样品的毒素含量已经超过食用安全标准。通过高效液相色谱法分析了有毒虾夷扇贝体内的毒素成分,共检出了6种麻痹性贝毒组分,主要以毒性较低的C1和C2毒素为主,GTX3和GTX2次之,STX和neoSTX含量很低。通过高效液相色谱法分析得到的各毒素组分毒性总和与小白鼠生物测试法毒性测试结果基本相当。     

黄河流域2002年麦收期间一次强降水过程分析

本文通过对陕西南部的佛坪县2002年6月8日——lO日的降水达到了489mm的特大暴雨降水过程的初步分析，对进一步了解大尺度环流背境场下的强降水过程的成因，提高对暴雨天气过程的预报能力是非常有益的；本文收集的降水过程期间的天气图和传真图资料对预报员和海洋工程技术人员有参考价值。     

青岛近海地区SO2和SO42-干沉降速率的季节变化

用小麦岛和团岛观测站提供的青岛近海气象和水温资料,以适于SO2气体和硫酸盐(SO42-)气溶胶的干沉降模型研究了模型中的输送阻尼对两种污染物质干沉降速率的贡献,并计算了2003年青岛近海地区两种污染物质干沉降速率的季节变化。结果表明:对SO2而言,粘性副层传输系数对沉降速率的贡献大于空气动力学传输系数;对于SO42-,空气动力学传输系数对沉降速率的贡献要大于表面传输系数。SO2和SO42-的干沉降速率变化范围分别为0.187~0.868cm/s和0.188~0.532cm/s。两种污染物质干沉降速率的四季变化有相似的规律,即冬季>秋季>春季>夏季。     

栉江珧工厂化育苗技术研究

通过亲贝强化营养和升温促熟培育,提早栉江珧人工育苗时间;合理控制幼虫密度,前期4~5个/mL,后期2~3个/mL,严把饵料质量关,投喂新鲜无污染的饵料,科学换水与充气,及时分级筛选等系列技术措施,克服栉江珧育苗中易发生幼虫粘连的技术难题,使育苗生产顺利进行;开展了多种附着基投放的采苗方法试验,选出了铺砂浮动网箱采苗和网袋装网片附着基和细砂吊在池中采苗2种比较理想的采苗方法,提高稚贝的附着变态率,其附着变态率达30%以上,促进稚贝的生长和成活,在1 000 m3水体中育出2 mm以上的稚贝1.096亿粒。     

黄茅海河口湾现代动力地貌体系和冲淤过程分析

1980—1993年对黄茅海河口湾进行沉积物采样和水流测定及水深测量。根据水动力和地形条件，冲淤分析及Mclaren模型研究河口湾的动力地貌体系、冲淤特征和现代沉积物运移。结果表明：（1）水下地形主要为下泄流或上溯流控制的“深槽－槽沟－浅滩－湾口”的动力地貌体系，反映了河口湾“东进西出”的水流格局；（2）整个河口湾以淤积为主，只有崖门深槽有较明显的优势冲刷特征，并随着崖门深槽向海推移和河口湾“东进西出”水动力作用，黄茅海落潮三角洲相应向西南进积；（3）应用Mclaren模型揭示了黄茅海河口湾现代沉积物运移规律，同样反映了河口湾具有“东进西出”的运移趋势。     

不同水温对文昌鱼性腺发育的影响

研究表明，不同水温对文昌鱼性腺发育分化和成熟有十分显著的影响。１４℃对文昌鱼性腺发育没有显著的作用，２０和２５℃十分有利于性腺发育和成熟，经４０ｄ培养，出现一些雌、雄文昌鱼产卵和释精以及加速性腺发育。本文还讨论了水温促进性腺发育可能的机制。     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.