Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplifi cation to related species

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this ?shery resources. The recently developed speci?c-locus ampli?ed fragment sequencing(SLAF-seq) is an effcient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites(mono-to hexa-nucleotide repeats) were identi?ed using SLAF-seq technology, of which di-nucleotide was the most frequent(13 590, 47.02%), followed by mono-nucleotide(8 138, 28.15%), tri-nucleotide(5 727, 19.81%), tetra-nucleotide(1 104, 3.82%), pentanucleotide(234, 0.81%), and hexa-nucleotide(112, 0.39%). One hundred and thirty-two microsatellite loci(di-and tri-nucleotide) were randomly selected for ampli?cation and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity( Ho) and expected heterozygosity( He) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and0.836, respectively. The polymorphism information content(PIC) ranged from 0.341 to 0.941(mean=0.806).However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in ?ve phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-ampli?ed in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis.The present microsatellites provided the ?rst known set of microsatellite DNA markers for D. maruadsi,D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the ?sheries management formulation and implementation of the understudied species.     

Responses of runoff to climate change and human activities in the Ebinur Lake Catchment,western China

Based on hydrological and climatic data covering the period from 1961 to 2008, this paper studies the hydrological responses to climate change and to human activities in the Ebinur Lake Catchment. The results show that the annual runoff of three rivers in Ebinur Lake Catchment exhibited different change trends. Specifically, in Jinghe River and Kuytun River exhibited a slightly increasing trend, but an adverse trend in Bortala River, and the variation trend has been the most dramatic since the mid-1990s. The observed variation in the runoff was resulted from the elevated alpine precipitation, rather than rising temperature, and that precipitation is a major factor for runoff generation. The runoff CAR model proposed by this paper can be used to predict the annual runoff in three rivers, and demonstrated annual runoff in Bortala River and Jinghe River will display an increased trend, while a less decreasing trend in Kuytun River under the climate change scenarios of warm-humid variation. In addition, the exploitation of the area of cultivated land led to more water resources consumption, primarily for agriculture irrigation, is the cause of the persistently ecoenvironment degradation, which have reached in a critical state thus, a more pressing concern is the development a scientifically reasonable and administratively practical water resource management scheme.     

钻孔后压浆灌注桩承载力试验研究

介绍了钻孔后压浆灌注桩施工工艺和加固机理,并根据原位测试结果分析了钻孔后压浆灌注桩荷载传递规律和大面积堆载试验对承载力的影响。     

河南省实施农业产业化思路与对策

文章结合河南省农业发展现状 ,分析了河南省实施农业产业化的必要性 ,指出实施农业产业化是河南省农村生产力发展的内在要求 ,是加快河南省农业经济增长、促进农村经济跃上新台阶的有效途径 ,并对河南省实施农业产业化的具体对策进行了探讨     

Response of vegetation NDVI to climatic extremes in the arid region of Central Asia: a case study in Xinjiang,China

Theoretical and Applied Climatology - Observed data showed the climatic transition from warm-dry to warm-wet in Xinjiang during the past 30 years and will probably affect vegetation...     

在Winkler法测定氧含量过程中生产绝对标准化水的新仪器

本文描述了一套新仪器,利用该仪器,在一定温度下与饱和空气的水汽逆流而达到平衡,这种方法在Winkler法氧测定中被认为是绝对标准的,Klots和Benson得出的新饱和值在15～30 ℃温度范围内得到证实,重复样品的氧含量标准偏差为0.005×10-3,在化学滴定法允许的误差范围以内.     

中国大陆科学钻探（CCSD）主孔100-2000m区间磁化率的变异特征及其地质意义

对中国大陆科学钻探主孔100—2000m区间岩心样品磁化率与钻孔原位测井磁化率进行系统时比研究。结果表明,控制岩石磁化率的主要因素是岩性和退变质作用,其中蛇纹石化石榴石橄榄岩的磁化率最大,样品平均磁化率为86．277×10-3SI,正片麻岩,退变质榴辉岩,榴辉岩,副片麻岩次之,角闪岩的磁化率最小,样品平均磁化率为1．698×10-3SI。不同退变质程度榴辉岩的磁化率特征具有明显的差异:中-强程度退变质榴辉岩由于退变质过程所产生的磁铁矿和钛铁矿-钛磁铁矿,其磁化率最大;随着退变质作用的进一步加剧,这些磁铁矿和钛铁矿-钛磁铁矿被完全分解,导致完全退变质榴辉岩的磁化率最小;新鲜榴辉岩的磁化率介于两者之间。在该深度区间,约70％岩心样品磁化率与测井磁化率的比值小于4．0,其中以<2．0为主,相同岩性岩石(如榴辉岩,退变质榴辉岩和副片麻岩)磁化率的比值随深度变化较小。岩心样品磁化率和测井磁化率之间的欧氏距离位于3．0-6．4范围,且随深度变化不明显,如在100～600m和1500～2000m两个深度范围的欧氏距离分别为3．0-5．2和3．2～5．3,表现出相似的变化特征,显示了在此区间深度范围内,地层压力对磁化率的基本没有影响。     

甘肃中强震前地磁“低点位移”现象研究

利用甘肃及邻近地区地磁垂直分量日变化极小值时间(低点时间)资料,分析了1995～2003年地磁"低点时间"在空间分布上的异常特征,即地磁"低点位移"现象,发现地磁"低点位移"现象除了有其自身的变化规律外,还与该区中强震的发生存在较好的相关性.1995～2003年的9年间,甘肃省中强震前均出现地磁"低点位移"现象.这表明地磁"低点位移"对该区地震的短临预测预报有一定的参考价值.