Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

Shrimps( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight(11.10 vs 10.50 g), body length(107.23 vs 106.42 mm), survival rate(67.80% vs 66.40%), and yield(6.42 vs 5.99 kg/m 3), and lower feed conversion ratio(1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable(10 4 –10 6 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component(75.7% of total input), shrimp feed(21.0%), water(2.06%), and shrimp biomass(1.22%). The major outputs of Cu occurred via the mechanical filter(41.7%), water renewal(15.6%), and draining of the sediment trap(15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems(30.70 mg/kg wet weight) was significantly higher than that in control systems(22.02 mg/kg). Both were below the maximum permissible concentration(50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.     

Change analysis of rice area and production in China during the past three decades

Rice＇s spatial-temporal distributions, which are critical for agricultural, environ- mental and food security research, are affected by natural conditions as well as socio-eco- nomic developments. Based on multi-source data, an effective model named the Spatial Production Allocation Model （SPAM） which integrates arable land distribution, administrative unit statistics of crop data, agricultural irrigation data and crop suitability data, was used to get a series of spatial distributions of rice area and production with 10-km pixels at a national scale -it was applied from the early 1980s onwards and used to analyze the pattern of spatial and temporal changes. The results show that significant changes occurred in rice in China during 1980-2010. Overall, more than 50% of the rice area decreased, while nearly 70% of rice production increased in the change region during 1980-2010. Spatially, most of the increased area and production were in Northeast China, especially, in Jilin and Heilongjiang; most of the decreased area and production were located in Southeast China, especially, in regions of rapidly urbanization in Guangdong, Fujian and Zhejiang. Thus, the centroid of rice area was moved northeast approximately 230 km since 1980, and rice production about 320 km, which means rice production moved northeastward faster than rice area because of the significant rice yield increase in Northeast China. The results also show that rice area change had a decisive impact on rice production change. About 54.5% of the increase in rice pro- duction is due to the expansion of sown area, while around 83.2% of the decrease in rice production is due to contraction of rice area. This implies that rice production increase may be due to area expansion and other non-area factors, but reduced rice production could largely be attributed to rice area decrease.     

河北唐山地区中寒武统张夏组鲕粒灰岩的岩石学研究

本区张夏组鲕粒灰岩内,广泛见到细径放射鲕和较粗的同心-放射鲕.放射鲕中存在连生体,我们称之“连生鲕”.与赵震等(1984)人工合成的静水鲕对比,区内放射鲕亦系静水条件下生成;因未见长串连生体,仅呈现数鲕(2—4个)连生,表明鲕粒生成后遭到搅动水拆开.由此可以认为,本组鲕粒灰岩属间歇高能成因模式的产物。又按该类岩石显示的成岩特征,可知本区鲕粒灰岩经历过多种成岩环境,至今仍保留较为明显的大气成岩环境的标志.     

基于UTC（NTSC）的GPS定时接收机时延测量

GPS提供了一种精密定时的方法,但接收机绝对时延的测量是一个难题,现有的测量方法成本高且难以操作,因而提出了一种GPS定时接收机时延测量的简单方法。这种方法根据国家授时中心保持的时间尺度UTC（NTSC）与GPS定时接收机输出的秒脉冲信号（1PPS）的比对结果,利用UTC（NTSC）的国际比对数据以及UTC与GPST的时差数据,可以测量GPS定时接收机时延,该方法简单易行。最后,对这种方法的测量误差进行了分析。     

Researches on the Internal Molecular Weight Uniformity of Chitosan Biomaterials

Uniform molecular weight(Mw)chitosan(CS)is highly demanded in medical biomaterial industry.This present article described heterogeneous degradation of CS in aqueous HCl/ethanol solution,in which progress uniform Mw CS was successfully prepared.The Mw distribution of CS was measured by gel permeation chromatography(GPC)analysis.Moreover,the structure and properties of degraded CS were characterized by Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance spectroscopy(^1H NMR),X-ray diffraction(XRD)and thermogravimetric(TG)analysis.In addition,the biocompatibility of degraded CS was also assessed by hemolysis rate(HR)measurement.The Mw of CS dramatically decreased from 246 KDa to 76 k Da at the initial 30 min,and stabilized at 18 kDa after 24 h.GPC analysis results showed that the degraded CS molecular become homogenization.FT-IR and 1 H NMR analysis confirmed the basic structure of CS molecular backbone was not destroyed during this progress.Besides,the water solubility of CS was not significantly influenced by this reaction.Moreover,the XRD analysis revealed that crystallinity of degraded CS increased from 70.32% to 99.25%with time.The TG analysis showed improved thermal stability of degraded CS.HR measurement demonstrated the degraded CS possessed excellent biocompatibility.This simple and efficient heterogeneous degradation would open up a new route to produce uniform Mw CS.     

Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata (Eustigmatophyceae)

1　Introduction　Nannochloropsissp .areoneofpotentialsourcesofpolyunsaturatedfattyacids ,especiallytheeicosapen taentaenoicacid (EPA ,C2 0∶5 ) ,whichisimportantforbothanimalsandhumans .　Thefattyacidcompositionofthisalgaisaffectedbyvariousenvironmentalfactors ,suchaslightintensity(Sukeniketal.,1989;Sukeniketal.,1990 ) ,imply ingthatafactorinfluencingthephotosyntheticprocessmayaffectcellularfattyacidsynthesisandtheirmetabolism .AselectionprogramofincreasingcellularEPAcontenthasbeencarriedo…     

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补肾生血方对肾性贫血大鼠EPO水平及EPOR mRNA的影响

目的：研究补肾生血方对肾性贫血大鼠促红细胞生成素（EPO）及EPOR mRNA水平的影响。方法：Wistar大鼠50只,分为对照组、模型组、中药组、EPO组、中药+EPO组,每组各10只,对除对照组外的其余组大鼠通过腺嘌呤灌胃制备肾性贫血大鼠模型,分别给予相应药物进行干预。分别于给药第2、8周观察血常规指标（Hb、RBC、HCT）、肾功能指标（BUN、Scr）的水平及EPO浓度,给药8周后处死大鼠,提取骨髓单个核细胞,测定EPOR mRNA表达量的变化。结果：与治疗2周相比,治疗8周中药组、EPO组、中药+EPO组大鼠的BUN、Scr显著降低,Hb、RBC、HCT、EPO水平及EPOR mRNA表达量显著上升,差异均有统计学意义（P＜0.05）。结论：补肾生血方具有改善肾性贫血模型大鼠肾功能、肾性贫血状况,其机制可能与提高骨髓有核细胞EPOR mRNA表达量,减轻毒素对EPOR的抑制,加强对内源性EPO反应性有关。     

FHD-2B型质子磁力仪数据采集故障原因分析

FHD-2B型质子磁力仪是按照中国地震局"十五"规程研制的地震前兆观测仪器,它通过IP接口接入前兆服务器,完成数据自动采集.通过分析该仪器在数据采集过程中出现空值的问题,总结从仪器回传数据的方法,并提出远程控制解决方案.     

南极半岛邻近海域夏季POC分布特征及其影响因素

海水中的颗粒有机碳(POC)与生物的生命过程、初级生产力关系密切,是海洋食物链中重要的物质基础和能量来源,因此POC的分布特征可以有效反映其生物地球化学环境。利用中国第33次南极考察期间(2016年12月至2017年1月)在南极半岛邻近海域采集的海水颗粒物样品,研究POC的空间分布特征及其影响因素。结果表明,斯科舍海0—200 m的POC浓度范围为7.44—193.52μg·L~(-1),平均浓度为(48.84±35.09)μg·L~(-1);南斯科舍海岭0—200 m的POC浓度范围为9.13—62.17μg·L~(-1),平均浓度为(29.76±14.12)μg·L~(-1);鲍威尔海盆0—200 m的POC浓度范围为5.87—270.72μg·L~(-1),平均浓度为(48.57±38.92)μg·L~(-1)。表层POC高值出现在斯科舍海区和鲍威尔海盆区,而低值出现在海岭区,与叶绿素a(Chla)的变化趋势一致,与营养盐的变化趋势相反。垂向分布上,各个区域POC平均浓度随深度的增加而减少,鲍威尔海盆和斯科舍海POC最高值都出现在25 m层。分析结果表明光合浮游植物是研究海域POC的主要来源, POC的主要影响因素为温度、水团混合以及海冰环境。斯科舍海与鲍威尔海盆整体非生命POC占比高,可能是由于高磷虾生物量、海冰碎屑以及陆源输入的干扰;南斯科舍海岭整体非生命POC占比低。