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101.
102.
103.
Dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A. 《中国海洋湖沼学报》2003,21(4):293-304
Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified
by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation.
In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction
after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively.
Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared
to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum
at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant
lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin
stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously,
ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in
holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production
by these cells may be one of the mechanisms of antibacterial protection of holothurians.
This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949). 相似文献
104.
地倾斜台站数字化、模拟观测数据频率特性研究 总被引:2,自引:2,他引:0
分析了四川、云南的部分地倾斜数字化观测与模拟观测资料的特性,从频率范围,周期10天以上的FFT谱特性,固体潮波振幅,相位特征值等方面,论述了地倾斜数字化资料较模拟记录资料的信息组成变化,并对资料的分析与应用进行了初步探讨。 相似文献
105.
1StrongtideandastronomicalconditionsPartial solar eclipse occurred 4 times in 1964, 1982 and 2000 respectively. Time interval is about 3 Saros periods (one Saros period is 18 years and 10.33~11.33 days). Total lunar eclipse occurred 2 times in 1964 and 2000 respectively and 3 times in 1982. However, there was no lunar eclipse in 1966, 1984 and 2002. It seems that they had similar astro-nomical conditions and the best was in 1982. The studies about the effect of tide on the global climate… 相似文献
106.
Zhang Qing Wang Li-fan Hu Jing-yao P. A. Mazzali Wang Zhen-ru 《Chinese Astronomy and Astrophysics》1995,19(4):426-431
We present the modeling of the ultraviolet and optical spectra obtained simultaneously on 1993 April 15 with the HST and at Lick Observatory. A Monte Carlo code is employed in the modeling and a comparison is made between models reported by different groups. With an atmosphere similar to the Sun in chemical composition, the observed spectral lines are well reproduced by a power law density structure of index around 20 except the strong H and HeI λ5876 lines which have peculiar absorption profiles. The photospheric velocity is found to be 9500 km/s and the blackbody temperature of the spectrum is 7990 K. For H and HeI λ5876, we suggest a two-component density structure which has a smoother layer located immediately outside the steeply decreasing inner envelope. The power law indices are most probably 20 and 3, respectively, with the transition point at about 13 000 km/s. In addition, this outer smooth layer serves to flatten the far UV spectrum as observed. 相似文献
107.
I.IN~crIOXLocatedinthecoddlepatofTallmBasin,withanareaof33.76X104klnZ,theTaldirnakan~isinthehinterlandofEurasia.Blockedbythehighmountainsaround,vapourofoceancanhardlyreachthedesert.APartfromthis,asthedeSertisinthesinkingcompensationareaoftheascensionalaircurrentaamstheQinghal-XIZangPlateau,itSprecipitationisrareandtheevaporationcapacityisintensealltheyearround.Allthesecontributetotheformationsofthetypifydrydesertclimate.Theacidityindexofthedesertandthearoundregionisashighasmorethan50,w… 相似文献
108.
Four bromophenols were isolated from the extract of marine red alga,Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods inclding IR, MS, 1D, and 2D-NMR techniques,
their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) bis (2,3-dibromo-4,5-dihydroxybenzyl)
ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4) 3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first
isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549
cells, 2 showed powerful antibacterial activities againstStaphylococcus aurens andPseudomonas aeruginosa.
Supported by National “863” Program (No. 2004AA625030, 2001AA620503), Quingdao marine sciences project (No. 04-2-NN-26) and
Key Knowledge Innovative Project of the Chinese Academy of Sciences (KZCX3-SW-215). 相似文献
109.
含有多重极点的有理真分式展开为部分分式,不需要计算低阶导数。列出被求函数的各阶导数的关系式,画出各阶导数作为节点的信号流图。该图无环路,既直观表现出各高阶导数的相互关系及影响的单向性,也使求节点增益的梅森公式特别简便。该方法将求高阶导数问题简化为代数运算,特别适合于编制成计算机程序 相似文献
110.
ZHANG Jimin ) LIU Shuang ) SUN Xue ) YANG Guanpin ) ZHANG Xuecheng ) * GAO Zhenhui ) ) North China Sea Monitoring Center SOA Fushun Road Qingdao P..R.China) College of Marine Life Sciences Ocean University of China Qingdao P..R.China 《中国海洋大学学报(英文版)》2003,2(1)
1 Introduction Nannochloropsissp .areoneofpotentialsourcesofpolyunsaturatedfattyacids ,especiallytheeicosapen taentaenoicacid (EPA ,C2 0∶5 ) ,whichisimportantforbothanimalsandhumans . Thefattyacidcompositionofthisalgaisaffectedbyvariousenvironmentalfactors ,suchaslightintensity(Sukeniketal.,1989;Sukeniketal.,1990 ) ,imply ingthatafactorinfluencingthephotosyntheticprocessmayaffectcellularfattyacidsynthesisandtheirmetabolism .AselectionprogramofincreasingcellularEPAcontenthasbeencarriedo… 相似文献