麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。     

花尾胡椒鲷养殖群体遗传多样性的等位酶研究

应用聚丙烯酰胺凝胶电泳（PAGE）技术对取自厦门火烧屿海水网箱的花尾胡椒鲷人工鱼养殖群体的遗传多样性进行等位酶分析。结果表明：所检测的16种同工酶的24个基因座位中，在XDH和GCDH基因座位上发现多态性，其平均多态位点比例为8．33％，基因座位有效等位基因的平均数Ne为1.083，平均杂合度的观测值Ho为0．00556，预期值He为0．00537,Hardy-Weinberg遗传偏离指数（D)为0．035。与其他鱼类相比较说明了其遗传多样性处于较低水平，文中对此进行了探讨并提出相应的种质资源管理保护措施。     

黄河口附近海域化学需氧量和石油烃分布及其关键控制环境因子分析

于2009年12月、2010年5月、2011年9月、2013年5月和2014年10月,在黄河口附近海域进行了5个航次调查,研究了该海域的化学需氧量(COD)和石油烃的分布情况以及季节变化。结果表明,COD整体呈冬季低、春季高的趋势,而石油烃无明显季节变化趋势。平面分布上,春季和秋季COD高值主要分布在靠近黄河口海域。春季和冬季石油烃高值主要位于调查海域的东北部区域。相关分析结果表明盐度、温度、pH、悬浮物、溶解氧及磷酸盐是影响COD的主要环境因素,而石油烃主要受盐度、温度、pH、无机氮、磷酸盐的影响。进一步多元回归分析结果表明,p H、盐度和溶解氧是影响COD的关键因子,温度是影响石油烃的关键因子。     

Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

Numerical simulation of solitary wave scattering by a circular cylinder array

The interaction of a solitary wave with an array of surface-piercing vertical circular cylinders is investigated numerically. The wave motion is modeled by a set of generalized Boussinesq equations. The governing equations are discretized using a finite element method. The numerical model is validated against the experimental data of solitary wave reflection from a vertical wall and solitary wave scattering by a vertical circular cylinder respectively. The predicted wave surface elevation and the wave forces on the cylinder agree well with the experimental data. The numerical model is then employed to study solitary wave scattering by arrays of two circular cylinders and four circular cylinders respectively. The effect of wave direction on the wave forces and the wave runup on the cylinders is quantified.     

人工饲料饲养的对虾肠道菌群和水体细菌区系的研究

对人工饲料饲养的凡纳滨对虾Litopenaeus vannamei成虾肠道菌群组成及其水体细菌区系组成进行了研究.分离纯化后的革兰氏阴性菌鉴定到种的水平.对虾肠道菌群主要由弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、嗜氢菌属Hydrogenophaga spp.、伯克霍尔德氏菌Burkholderia spp.、气单胞菌Aeromonas spp.、食酸菌Acidovorax spp.和芽孢杆菌Bacillus spp.组成.水体细菌区系主要包括弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、艾肯菌Empedobacter spp.、无色杆菌Achromobacter spp.和芽孢杆菌Bacillus spp..肠道菌群和水体细菌区系的优势菌是副溶血弧菌Vibrio parahaemolyticus和芽孢杆菌Bacillus spp..利用Shannon-Wiener指数进行多样性分析,发现虾肠道多样性指数（H）和均匀度（J）高于水体细菌区系,而丰度（D）较低.     

Artificial upwelling induced by ocean currents —Theory and experiment

A submerged apparatus, which consists of a buoy, several horizontal contraction and expansion tubes (Venturi-type tubes) and a long pipe, is expected to be used to pump the subsurface sea-water (200–300 meter depth) containing abundant nutrients to surface layer (50–100 m) by the dynamic of ocean currents. i.e. an artificial upwelling without energy cost. A preliminary experiment and analysis are undertaken and shows that the capacity of pumping the nutrient-rich sea-water is worth to build a pilot prototype model.     

水下智能潜器的神经网络运动控制

本文介绍一种基于神经网络的水下智能潜器的运动控制方法，该方法通过在线学习，融控制与滤波为一体。计算机仿真与水池实验验证表明，该方法的控制与滤波性能良好，对环境的学习与适应能力强。该方法事实上可用于一般动力系统的控制。     

A STUDY OF MICROTEKTITES IN THE PACIFIC OCEAN

In 1979, we found many small colorless transparent and light yellow-green glassy spherules in the surface layer and core sediment samples taken from the west of the Central Pacific Occan, which are considered to be microtektites. This finding is worthy of further determination of the range of strewn field and origin of tektites.     

Resistant performance of perforation of multi-layered targets using an estimation procedure with marine application

Recently, multi-layered targets have become commonplace in both military and civilian applications, such as marine hulls, armored vehicle bodies, outside structures of bulletproof cars, and aerospace vessels. This paper studies the resistant performance of perforation in multi-layered targets. An estimation procedure is established based on the concepts of the conservation of momentum, impulse-momentum law, and conservation of energy. Experimental results of the test of Almohandes et al. are adopted to check the residual velocity of multi-layered targets. The results of the verification are good in terms of agreement for impact velocities ranging from 700 to 800 m/s, when the ratio of the projectile length to the projectile diameter (i.e. L/D) is 4.2, and the average residual velocity error of single, double- or triple-layered targets range approximately from 4.42 to 8.40%. The ballistic performance is best for the double target when the ratio of the first layer thickness to the total thickness (i.e. t₁/(t₁+t₂)) is about 0.75, and the worse performance occurs when the ratio t₁/(t₁+t₂) is 0.5. An air gap slightly influences the resistant performance of perforation in multi-layered targets. These results may serve as a useful reference for designers.