Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

兖州市加快合村并点保障经济社会发展

近年来,兖州市坚持以科学发展观为指导,着眼于集约节约用地,以实施合村并点、挂钩试点为突破口,大力推进村庄整理,全市已经改造整理村庄38个,节约土地300hm^2,并计划结合村庄合并建设50多个中心社区,可节约土地0．2万余公顷。     

Causes of rockfalls in Al-Huwayshah area, Yemen

According to topography of Yemen, most areas and villages are located at obligated crest, toe of mountain and under cliffs. Therefore Al-Huwayshah consisting of Tawilah sandstone group is characterized by steep slope reach to 90° in some areas. This area is affected by strong tectonic movements and faults that occurred during the geological epochs. This effect enhances to find out fractures and joints as well as the rocks become brittle and ready to slide depending on the position of area. And there are some fractures and joints on the surface of the areas due to tectonic movement associated with opening the Red Sea and Gulf of Aden. The rainfalls, fractures, joints, earthquakes, gravity, vegetations, temperatures and human activities play a big role in the processes of rockfalls and landslides in this area. All those factors are considered as the basic causes and catalyzed factors for occurrence of rockfall in the studide area. In addition, the authors carried out laboratory test for many rock samples to get the physical properties of the rocks.     

乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构

利用透射电子显微镜观察乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构。结果表明,乌苏里拟鲿精子由头部、中段和尾部组成。头部呈球形或椭圆球形,核前端无顶体,后端有植入窝。中段包括中心粒复合体和袖套。植入窝从细胞核的后端陷入核中。中心粒复合体位于植入窝内,包括近端中心粒和远端中心粒。袖套与细胞核后端相连,袖套中含有丰富的线粒体。尾部主要结构是轴丝,为典型的"9+2"结构,轴丝外两侧为细胞质膜形成的对称性鳍样结构。尾部内无囊泡及其他细胞器。     

Promoting Mieropedology

The Journal of Mountain Science is a multi-disciplinary scientific journal that covers research in earth science, geography, environmental sciences and life sciences. This special issue recognizes the importance of micropedology, a field that is often overlooked. The 14 papers published here were selected from the 135 presented at the 13^h International Conference on Soil Micromorphology （ICSM） that took place on September 11-16, 2008, in Chengdu, China. These articles review the history and development of soil micromorphology, and highlight the current status of the field right up to the latest cutting edge research, thus providing readers excellent access to the entire micropedologv literature.     

黄海南部黄鲫(Setipinna taty)资源利用分析

以2005-2006年江苏省帆式张网渔获资料为基础,分析研究了黄海南部黄鲫资源的时空分布、群体组成结构以及产卵群体生物学特征.结果表明,黄海南部黄鲫主要利用渔期为春季3-5月、冬季1月或12月.春季高产渔区主要分布在大沙渔场的西南部和吕泗渔场的东部,冬季和秋季高产渔区相对靠近或较春季高产渔区偏东.黄鲫高产渔区的分布无明显季节变化特征.黄鲫渔获群体主要由体长70-110mm、体重0-15g的个体组成,产卵群体的平均体重范围为8.7-12.0g.与1981-2000年的研究结果相比,黄鲫个体小型化越趋显著,当前利用个体偏小.     

Baja California 边缘PC14 岩芯海水表层温度及生产力变化的百年尺度记录

加利福尼亚流是北太平洋环流的重要组成部分,它的变化对全球气候及碳循环有重要影响.对位于东北太平洋低纬区BajaCalifornia外海的PC14柱状样进行了有机地球化学分析,重建了过去14ka来这一地区百年尺度的海水表面温度及生产力变化,发现其存在明显的B/A(Bling-Aller)和YD(Younger Drays)事件,但B/A和YD事件温度变化幅度小于中纬度地区.与中纬度加利福尼亚流系早中全新世温度降低不同,PC14全新世温度无明显变化趋势.这些结果表明了东太平洋低纬地区对高纬地区的气候响应以及沿岸上升流和ENSO现象对加利福尼亚流系中纬和低纬地区的影响不同.从冰消期到全新世,PC14生产力呈现阶段上升趋势,指示了由E1 Nino气候向La Nifia气候的转化以及沿岸上升流加强的总体趋势.在全新世中后期(6.5-3.8ka)生产力的降低,则可能是受ENSO加强的影响.在B/A暖期,PC14生产力增加不多,但氧最小层强度增加,这表明生产力变化不是东太平洋边缘海的氧最小层强化的控制因素.     

泥鳅(Misgurnus anguillicaudatus)病原霍乱弧菌(Vibrio cholerae)的表型与分子鉴定

从江苏连云港某养殖场养殖死亡的泥鳅肝脏、血液及腹水中分离出大量优势生长的细菌,人工感染试验证明其对泥鳅具有很强的致病性。采用表观分类学及分子生物学方法,对分离菌进行了形态特征、理化特性、胞外酶活性及溶血活性等生物学性状检验;用PCR方法同时扩增其16SrRNA和gyrB基因,分析了16SrRNA和gyrB两种基因序列的同源性,并构建了系统发生树,通过基因序列分析,比较了两种基因在相似细菌的检测和鉴定能力;基于16SrRNA和gyrB基因的系统发育学分析表明分离菌(LD081008B-1)所扩增的16SrRNA和gyrB基因序列均与GenBank数据库中霍乱弧菌具有较高的相似性,且gyrB基因用于细菌种间鉴定更具优越性;16SrRNA基因序列长度为1446bp(GenBank登录号:GQ205447),gyrB基因序列长度为1207bp(GenBank登录号:GQ205452);根据分离菌的表型特征及分子特征,判定病原菌为弧菌属(VibrioPacini1854)的霍乱弧菌(Vibriocholerae)。胞外酶活性及溶血活性检测表明分离菌均具有蛋白酶、卵磷脂酶、淀粉酶、明胶酶及DNA酶活性,在含7%家兔脱纤血...     

长春市区地基土的动力性能及地震危害分析

在统计资料基础上,根据市区地基土的类型,对设防烈度在7度下土的动力性能作了探讨,在此基础上划分了场地类别。     

台风作用下淤泥质海岸动力地貌响应

基于200509号麦莎台风(近似百年一遇)作用前后杭州湾北岸龙泉-南竹港实测的海岸地形和水文资料,利用分形技术、冲刷深度和闭合水深等相关概念分析淤泥质海岸的剖面变化、岸线进退以及地形冲淤,进而探讨海岸响应台风作用的动力地貌行为.结果表明:1)台风作用前后的海岸动力地貌过程表现为水下类似沙坝的堆积体削平、凹处填洼,水下平台发育锯齿或较小的坝--槽,海岸剖面的分维数降低,剖面坡度处于平缓;2)麦莎台风对海床的整体冲淤幅度不大,这可能是由于研究区大潮讯的增水致使波浪对海床的塑造能力减弱以及码头的遮蔽作用造成;3)台风作用对海岸的冲刷深度约为0.8 m,闭合水深为9.5 m,近岸-1 m邻近区成为海岸响应台风作用最敏感的地带;泥沙主要在0 m到闭合水深区间的海床发生纵、横向运移.