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101.
102.
Dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A. 《中国海洋湖沼学报》2003,21(4):293-304
Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified
by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation.
In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction
after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively.
Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared
to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum
at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant
lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin
stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously,
ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in
holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production
by these cells may be one of the mechanisms of antibacterial protection of holothurians.
This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949). 相似文献
103.
地倾斜台站数字化、模拟观测数据频率特性研究 总被引:2,自引:2,他引:0
分析了四川、云南的部分地倾斜数字化观测与模拟观测资料的特性,从频率范围,周期10天以上的FFT谱特性,固体潮波振幅,相位特征值等方面,论述了地倾斜数字化资料较模拟记录资料的信息组成变化,并对资料的分析与应用进行了初步探讨。 相似文献
104.
1StrongtideandastronomicalconditionsPartial solar eclipse occurred 4 times in 1964, 1982 and 2000 respectively. Time interval is about 3 Saros periods (one Saros period is 18 years and 10.33~11.33 days). Total lunar eclipse occurred 2 times in 1964 and 2000 respectively and 3 times in 1982. However, there was no lunar eclipse in 1966, 1984 and 2002. It seems that they had similar astro-nomical conditions and the best was in 1982. The studies about the effect of tide on the global climate… 相似文献
105.
应用拉格朗日插值法拟合鱼类体长与体重之间的函数关系 ,使用计算机VB语言进行编写程序 ,与常用的线性回归法、Ricker法进行比较 ,得出体长与体重的关系式为Pn(x) =∑nk=0yklk(x)=∑nk=0(∏nj=0j≠kx-xjxk-xj)yk 。 相似文献
106.
The new polyenzyme method for making gravy from Harengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50℃ , alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, andflavorase concentration of 2.0% , for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3 % of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5% , total and amino nitrogen being respectively1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy from Harengula zunasi offal. In addition, in inorganic elements, the phosphorus content is the highest. 相似文献
107.
智能照明系统在现代建筑照明中的应用研究 总被引:1,自引:0,他引:1
陈丹 《成都信息工程学院学报》2007,22(6):735-738
在对传统照明系统和智能照明系统进行对比分析的基础上,剖析了智能照明系统的控制策略,并结合实际的工程应用,着重讨论了基于ABB I-Bus产品的智能照明控制系统的实现,结果表明该系统具有高效、便捷、节约能源等优点.同时,还对系统与建筑物楼宇自控系统问的系统集成方式进行了研究. 相似文献
108.
介绍了基于STC89C516RD+单片机的实验开发平台的硬件电路设计、ISP编程、软件开发实例及系统的抗干扰措施.为帮助学生学习单片机技术,同时也为有经验者开发和调试产品打下基础. 相似文献
109.
以小杂鱼或低值鱼为原料 ,采用常温溶剂萃取法进行脱脂 ,研究了不同溶剂、溶剂混合比、流速和萃取时间对鱼类脱脂的影响。结果表明 ,在常温下用等体积的异丙醇和乙酸乙酯混合溶剂 ,以流速 4~ 8mL/min连续萃取 4~ 6h ,脱脂鱼肉的脂肪平均含量w为 0 .19%,脱脂率高达96 .2 6 %。该方法的溶剂损耗量为加热溶剂萃取法的 1/3倍。 相似文献
110.
Zhang Qing Wang Li-fan Hu Jing-yao P. A. Mazzali Wang Zhen-ru 《Chinese Astronomy and Astrophysics》1995,19(4):426-431
We present the modeling of the ultraviolet and optical spectra obtained simultaneously on 1993 April 15 with the HST and at Lick Observatory. A Monte Carlo code is employed in the modeling and a comparison is made between models reported by different groups. With an atmosphere similar to the Sun in chemical composition, the observed spectral lines are well reproduced by a power law density structure of index around 20 except the strong H and HeI λ5876 lines which have peculiar absorption profiles. The photospheric velocity is found to be 9500 km/s and the blackbody temperature of the spectrum is 7990 K. For H and HeI λ5876, we suggest a two-component density structure which has a smoother layer located immediately outside the steeply decreasing inner envelope. The power law indices are most probably 20 and 3, respectively, with the transition point at about 13 000 km/s. In addition, this outer smooth layer serves to flatten the far UV spectrum as observed. 相似文献