A new record of Platycephalus sp.1 (sensu Nakabo, 2002) was documented based on morphological characters and DNA barcoding. We collected 174 specimens of the genus Platycephalus from Chinese coastal waters of Dongying, Qingdao, Zhoushan, and Beihai. Samples were identified as Platycephalus sp.1 morphologically. The coloration, meristic traits, and morphometric measurements are consistent with previously published records. In brief, it is an orange-brown flathead fish with dark brown spots scattered on head and body, lateral line scales 83 to 99 with one or two spine-bearing anteriormost pored scale, no yellow blotch on the caudal fin. Cytochrome oxidase I subunit (COI) gene fragments were sequenced for phylogenetic analysis. The mean evolutionary distance within the species Platycephalus sp.1 was 0.1%. Net evolutionary distances between Platycephalus sp.1 and other species of Platycephalus ranged from 10.8% to 19.7%, which is much greater than the threshold for species delimitation. The COI sequence analysis strongly supports the validity of Platyceohalus sp.1 at genetic level. 相似文献
Vibrio fluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria
with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering
bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus
at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was
detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial
adhesion. Extracts of V. fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations
enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V. fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus,
and the environment. 相似文献
With increasing demands for coal resources, coal has been gradually mined in deep coal seams. Due to high gas content, pressure and in situ stress, deep coal seams show great risks of coal and gas outburst. Protective coal seam mining, as a safe and effective method for gas control, has been widely used in major coal-producing countries in the world. However, at present, the relevant problems, such as gas seepage characteristics and optimization of gas drainage borehole layout in protective coal seam mining have been rarely studied. Firstly, by combining with formulas for measuring and testing permeability of coal and rock mass in different stress regimes and failure modes in the laboratory, this study investigated stress–seepage coupling laws by using built-in language Fish of numerical simulation software FLAC3D. In addition, this research analyzed distribution characteristics of permeability in a protected coal seam in the process of protective coal seam mining. Secondly, the protected coal seam was divided into a zone with initial permeability, a zone with decreasing permeability, and permeability increasing zones 1 and 2 according to the changes of permeability. In these zones, permeability rises the most in the permeability increasing zone 2. Moreover, by taking Shaqu Coal Mine, Shanxi Province, China as an example, layout of gas drainage boreholes in the protected coal seam was optimized based on the above permeability-based zoning. Finally, numerical simulation and field application showed that gas drainage volume and concentration rise significantly after optimizing borehole layout. Therefore, when gas is drained through boreholes crossing coal seams during the protective coal seam mining in other coal mines, optimization of borehole layout in Shaqu Coal Mine has certain reference values.
Contributions to Mineralogy and Petrology - Fractionation of felsic magma has been critical for the formation of high-silica rhyolites, chemical differentiation of the continental crust, and... 相似文献