Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

Uncertainty representation of ocean fronts based on fuzzy-rough set theory

Analysis of ocean fronts' uncertainties indicates that they result from indiseemibility of their spatial position and fuzzi-ness of their intensity. In view of this, a flow hierarchy for uncertainty representation of ocean fronts is proposed on the basis of fuzzy-rough set theory. Firstly, raster scanning and blurring are carried out on an ocean front, and the upper and lower approximate sets, the indiscernible relation in fuzzy-rough theories and related operators in fuzzy set theories are adopted to represent its uncer-tainties, then they are classified into three sets: with members one hundred pereent belonging to the ocean front, belonging to the ocean front's edge and definitely not belonging to the ocean front. Finally, the approximate precision and roughness degree are util-ized to evaluate the ocean front's degree of uncertainties and the precision of the representation. It has been proven that the method is not only capable of representing ocean fronts' uncertainties, but also provides a new theory and method for uncertainty representation of other oceanic phenomena.     

基于NDVI城镇土地利用变化检测探讨

通过归一化植被指数（NDVI）结合影像差值,对经过辐射校正后的1993年和2005年武汉地区TM影像进行了土地利用变化检测,与监督法分类进行比较,得出NDVI更宜于实现变化信息探测和提取。     

张家界旅游企业空间成长对城镇空间形态的影响

运用核密度估计法和标准差椭圆分析法,分析张家界旅游企业空间成长特征及演化过程;选取紧凑度指标,结合城镇空间均衡分布椭圆,分析张家界城镇空间形态演化过程;通过比较不同阶段旅游企业椭圆和城镇空间椭圆的重合面积,剖析旅游企业空间成长对城镇空间形态的影响程度。研究发现：① 张家界旅游企业空间成长过程和特征为：由出现期的“一核多极点”逐渐演变为生成期的“核心-边缘”,最后在发展期形成一个高集聚中心和三个次中心联动的“组团扩展型”格局;② 张家界建成区面积呈现增长态势,城镇空间不断扩展,建成区用地紧凑度逐渐下降;③ 不同阶段,张家界旅游企业空间成长对城镇空间形态的影响程度有一定的差异性,整体呈现出逐渐增强的趋势。研究结论对于重新认识旅游对城镇化建设的作用具有重要的现实意义。     

1961—1987年与1988—2014年辽宁省气候区划界线变化及可能气候成因

基于同一区划方法、指标体系,使用1961—2014年辽宁省52站气象观测资料,分析辽宁省气温、气候区划指标、范围及界线的变动特征。结果表明：辽宁省年均气温在1988年发生一次突变,突变后气温开始显著上升;≥10 ℃积温日数比较显著地响应气温突变,而干燥指数、7月平均气温变化不显著。在空间分布上区划指标值均存在不同程度的变化。① 全省≥10 ℃积温日数均出现增加,但在中西部地区显著增加;② 在盘锦-抚顺一线以北（南）,气候总体呈不显著变湿（干）趋势;③ 7月平均气温呈缓慢上升趋势。区划范围及界线位置出现更加显著地变化：① 暖温带范围主要向北向东扩展,中温带向东收缩;② 半湿润区范围主要向北向西扩展,半干旱区向西北方向收缩,湿润区范围基本不变;③ Tb范围显著向北向东扩展,Ta范围向北向东收缩。在此基础上分析了气候格局变化的可能气候成因,发现突变后≥10 ℃积温日数期间500 hPa高度场增加与4月和10月东亚冬季风减弱,4—10月东北冷涡持续天数增加和7月500 hPa高度场增加,可能分别是温度带,Tb区、Ta区和半湿润区、半干旱区变化的原因。     

The evolution of the spatial-temporal patterns of global energy security since the 1990s

Journal of Geographical Sciences - In this study, we developed an energy security evaluation model (ESEM) from three dimensions, energy supply-transport security, safety of energy utilization, and...     

Benthic foraminiferal fauna and sediment provenance of Eocene syn-rift sequences in Taiwan: implication for onset of Asian epi-continental marginal seas off China coast

Marine Geophysical Research - Early Cenozoic rift basins developed commonly on the Mesozoic basement along the SE Asia Continent. However, Eocene–Oligocene sequences were only exposed widely...     

Jason-2高度计电离层GIM误差分析及修正研究

基于Jason-2高度计2015年地球物理数据集(GDR)38个周期太平洋海域的全球电离层图(GIM)电离层校正值和双频校正值的数据,分不同季度和不同纬度区域比较二者的差异,结果表明:GIM值与双频校正值之间存在明显的差异,GIM校正值普遍高于双频校正值,说明GIM高估了电离层路径延迟,GIM校正值与双频校正值的差异与季节和纬度区间有关。用梯度下降法得到GIM值的修正方程,将修正方程应用于2016年Jason-2的全年数据,修正后的GIM值与双频校正值十分接近,在各年份中均具有良好的适用性。在单频高度计不能使用电离层双频校正算法的情况下,可以利用不同季度和不同纬度区域的修正方程对同等高度的高度计GIM值进行修正以达到双频校正值的精度水平。     

珊瑚环礁区侧扫声呐图像中的目标物识别方法

为研究西沙宣德环礁海底地貌特征及珊瑚分布情况,采用舷挂侧扫拖鱼的方式对环礁区域海底进行地貌扫测,从采集的侧扫声呐图像上可以识别出环礁区域珊瑚分布情况及珊瑚礁区地貌特征,利用侧扫声呐图像识别方法结合实物样品比对可以圈定出珊瑚分布范围、暗礁区域及环礁区域底质类型分布,采用基本的数学处理方法定量分析了水下珊瑚礁体的海底高度及疑似沉船的大小,分析了侧扫声呐图像显示的调查船转向效应。