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391.
Metallothioneins (MT) are proteins that play an important role in metabolism of essential metals and detoxification of trace metals from living organisms. Their synthesis is induced by metal pollution but can also be exacerbated by other factors such as reproduction processes. In this context, we monitored MT concentrations in a cockle Cerastoderma edule (marine bivalve) population and highlighted the effect of a castrating digenean parasite, Labratrema minimus. In spent cockles, MT levels were low (ca. 5 nmol sites g(-1), fresh weight) but slightly higher in parasitized individuals. During gametogenesis, MT synthesis increased in all cockles, but concentrations were lower in parasitized individuals (18 against 27 nmol sites g(-1), fw in unparasitized cockles) in relation with gonad damage by parasites. Therefore, it is suggested that parasite infection in cockles can modulate MT synthesis that could consequently interfere with the response of these protective proteins in case of metal contamination. 相似文献
392.
Simulation of fracturing processes in porous rocks can be divided into two main branches: (i) modeling the rock as a continuum enhanced with special features to account for fractures or (ii) modeling the rock by a discrete (or discontinuous) approach that describes the material directly as a collection of separate blocks or particles, e.g., as in the discrete element method (DEM). In the modified discrete element (MDEM) method, the effective forces between virtual particles are modified so that they reproduce the discretization of a first-order finite element method (FEM) for linear elasticity. This provides an expression of the virtual forces in terms of general Hook’s macro-parameters. Previously, MDEM has been formulated through an analogy with linear elements for FEM. We show the connection between MDEM and the virtual element method (VEM), which is a generalization of FEM to polyhedral grids. Unlike standard FEM, which computes strain-states in a reference space, MDEM and VEM compute stress-states directly in real space. This connection leads us to a new derivation of the MDEM method. Moreover, it enables a direct coupling between (M)DEM and domains modeled by a grid made of polyhedral cells. Thus, this approach makes it possible to combine fine-scale (M)DEM behavior near the fracturing region with linear elasticity on complex reservoir grids in the far-field region without regridding. To demonstrate the simulation of hydraulic fracturing, the coupled (M)DEM-VEM method is implemented using the Matlab Reservoir Simulation Toolbox (MRST) and linked to an industry-standard reservoir simulator. Similar approaches have been presented previously using standard FEM, but due to the similarities in the approaches of VEM and MDEM, our work provides a more uniform approach and extends these previous works to general polyhedral grids for the non-fracturing domain. 相似文献
393.
In this paper, we study the use of virtual element method (VEM) for geomechanics. Our emphasis is on applications to reservoir simulations. The physical processes behind the formation of the reservoirs, such as sedimentation, erosion, and faulting, lead to complex geometrical structures. A minimal representation, with respect to the physical parameters of the system, then naturally leads to general polyhedral grids. Numerical methods which can directly handle this representation will be highly favorable, in particular in the setting of advanced work-flows. The virtual element method is a promising candidate to solve the linear elasticity equations on such models. In this paper, we investigate some of the limits of the VEM method when used on reservoir models. First, we demonstrate that care must be taken to make the method robust for highly elongated cells, which is common in these applications, and show the importance of calculating forces in terms of traction on the boundary of the elements for elongated distorted cells. Second, we study the effect of triangulations on the surfaces of curved faces, which also naturally occur in subsurface models. We also demonstrate how a more stable stabilization term for reservoir application can be derived. 相似文献