长牡蛎碱性磷酸酶的分离纯化及部分性质研究

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.     

高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用

通过自行改进的高压培养罐及高压设备,对深海沉积物进行可培养微生物的筛选,获得6株具有较强耐受压力的细菌.16SrDNA的测序结果表明这些细菌分别属于6个不同的菌属.压力生长试验的结果表明这6株细菌在40MPa的条件下仍然具有较强的生长能力,属于兼性嗜压菌.对不同压力下生长的细菌做显微镜检,结果显示,除了一株芽孢杆菌在40MPa下的菌体形态发生了明显的变化外,其它5株细菌在压力条件下的菌体的分裂均没有受到明显的影响.     

温度、pH对缢蛏(Sinonovacula constricta)消化酶活力的影响

本文研究了温度、pH对缢蛏(Sinonovacula constricta(Lamarck))三种主要消化酶(蛋白酶、淀粉酶和纤维素酶)活力的影响。结果表明：当温度在5～75℃之间时，蛋白酶、纤维素酶和淀粉酶的最适温度分别为为55℃、45℃和65℃；在不同的pH范围内，蛋白酶的最适PH值为9．2，淀粉酶的最适PH值为6．3和7．7，纤维素酶的最适PH值为5．2。缢蛏的淀粉酶和蛋白酶活力较大，纤维素酶活力极微。     

大亚湾海域锥状斯氏藻赤潮及其与孢囊的关系

用沉积物捕捉器取样，研究了大亚湾海域２０００年６～９月孢囊（Ｃｙｓｔ）种群动态，同时 分析了浮游植物种群动态。研究期间（８～９月）该海域发生了一起大规模的锥状斯氏藻赤潮。 赤潮前一个月，锥状斯氏藻孢囊的平均日形成率为２．０５× １０５ｃｙｓｔｓ／（ｍ２·ｄ），丰度为５０．２２％， 该时期以有性生殖产生的静止期孢囊为主；在赤潮盛期的８，９月初，孢囊的平均日形成率达到 ２．０１ ×１０６ｃｙｓｔｓ／（ｍ２·ｄ），丰度为９６．９７％，此时期的孢囊多为营养细胞所形成的暂时性孢囊； 赤潮结束后，由于环境条件的恶化，暂时性孢囊的形成达到高峰，平均日形成率为１．２４×ｌ０７ ｃｙｓｔｓ／（ ｍ２·ｄ），丰度为９９.５３％。结果表明大亚湾丰富的孢囊是潜在的种源（Ｓｅｅｄ　ｂｅｄ），２０００年 的夏季、秋李持续高温（＞２５℃），台风减少及风力减弱等有利锥状斯氏藻的环境条件诱发孢 囊萌发，开始了该种类的赤潮。     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。     

高精度实验室盐度计测量结果的不确定度分析

为了使高精度实验室盐度计的检定、校准工作更加科学、准确、合理,在此对高精度实验室盐度计验收过程进行分析,然后根据数据处理规则和JJF1059-1999《测量不确定度评定与表示》,对测量结果的不确定度进行了详细的分析和评定。     

黄海海域陆相中生界油气远景

黄海海域油气勘探已40余载，尚未实现油气突破，其找油前景引起石油地质学家的极大关注。根据前人的认识和最新研究成果，结合邻区油气地化资料，探讨了黄海海域中生界地层生、储、盖特征，特别是白垩系，它分布广，厚度大，烃源岩达到较好生油岩标准，并且生、储、盖组合理想，中生界是该区下部油气勘探首选目标。     

Gradation affects basic mechanical characteristics of Chinese calcareous sand as airport subgrade of reefs

Abstract

Construction of the reefs in the South China Sea is a significant foundation to the secure stability and economic development of China. The construction of an airport runway is necessary for this realization. The calcareous sand is the main primary material in the runway construction. A certain type of calcareous sand near a certain reef of the South China Sea was studied in this paper. To investigate this specific calcareous sand, quartz sand was used as a reference for comparison. Microscopic 3-D imaging, compression and triaxial tests were conducted to test the micro, squeezing and shear properties. The effect mechanism of gradation on the calcareous sand’s compressibility and shear characteristics are discussed from a mesoscopic viewpoint using 3-D morphology. Calcareous sand particles are multiangular and flatter in comparison with quartz sand. The larger the particle sizes are, the more different the two sands’ morphologies are. The compressibility of calcareous sand is greater, and the effect of the coarse fraction (5–1?mm) content in the gradation plays the most significant role in this feature. When the coarse particles’ content is less than 25% and the mass ratio of the middle and fine particles (M) is constant, there is the worst coarse fraction content causing the calcareous sand to be most likely compressed. The worst coarse fraction content decreases with the increase in M, and an empirical formula is proposed. When the gradation, relative density and confining pressure are the same, the peak shear stress and strain of calcareous sand are all at a high level. The effect of confining pressure is manifested in calcareous sand. The shear strength and dilation of calcareous sand are also most affected by the medium coarse fraction (5–0.25?mm) content.     

Parabolic Wave Propagation Modelling in Orthogonal Coordinate Systems

This paper presents a numerical model study of the propagation of water waves using the parabolic approximation of the mild-slope equation in the orthogonal coordinate system. Two types of coordinate systems are studied: (a) a general form of orthogonal coordinate system and (b) the conformal system, a special form of orthogonal coordinate system. Two typical examples, namely, expanded breakwaters and a circular channel, are studied to validate the model. First, the examples are studied by use of the general orthogonal coordinates. Then the same examples are computed by use of the confonnal system. The computational results show that the confonnal coordinate system generally gives better predictions than the general orthogonal system. A numerical technique for generating the conformal grid is combined with the numerical model to improve the practicability of the model. The comparison between the result from the numerical grid system and that from the analytical grid system shows that reliable computational results can be obtained by use of the numerical confonnal grid system.     

基于神经网络的平底结构砰击压力预报

对利用神经网络预报平底结构入水砰击压力的方法进行了探讨。首先利用仿真软件计算各种情况下平底结构入水所产生的砰击压力，以此形成训练神经网络的数据集。其次利用数据集对三层反馈式网络进行了训练，讨论了不同隐含层节点数对该非线性系统的拟合能力，并且对梯度下降法、动量修正法和基于优化的LM算法的有效性和精度进行了比较，最后得出了适合平底结构入水砰击系统的网络结构。