二色裂江珧EST和SOD同工酶组织特异性研究

取二色裂江珧 (Pinna bicolor)的消化盲囊、肾脏、后闭壳肌肌肉、外套膜和鳃等 5种组织 ,用垂直板聚丙烯酰胺凝胶电泳对酯酶 (EST)和过氧化物歧化酶 (SOD)同工酶在上述组织中的表达和分布进行了比较研究 ,并对酶谱表型及位点表达进行了分析。结果表明 ,二色裂江珧组织内的 EST和 SOD存在不同程度的组织特异性 ,EST共检测出 15条酶带 ,SOD检测出 8条酶带 ,且两种同工酶在消化盲囊中活性最高。推测 SOD由 2个座位编码 ,可分为 s- SOD二聚体和 m- SOD四聚体     

A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01

Sulfotransferase (ST) is the first enzyme discovered in association with paralytic shellfish poisoning (PSP) toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity in crude enzyme extraction of a toxic dinoflagellate species, Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3’-phosphoadenosine 5’-phosphosulfate (PAPS) to N-21 in the carbamoyl group of gonyautoxin 2/3 (GTX2/3) to produce C1/C2, but is inactive toward STX to produce GTX5. The crude enzyme is optimally active at pH 6.0 and 15°C. The activity is enhanced by Co2 , Mg2 , Mn2 and Ca2 individually, but is inhibited by Cu2 . Moreover, the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition, the ST from different dinoflagellates is species-specific, which explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.     

Soil evolution features of debris flow waste-shoal land

The reclamation and utilization of debris flow waste-shoal land plays an important role in the mitigation and control of debris flow hazards, which thus contributes a lot to the exploitation of insufficient land resources in mountainous areas and the reduction of losses caused by debris flow. The aim of this paper is to discuss the features and mechanism of soil evolution of debris flow waste-shoal land so as to search for the available modes of its reclamation and utilization. The Jiangjiagou Ravine, a typical debris flow ravine, was selected to study soil evolution features of debris flow waste-shoal land based on the analysis of soil physieochemical properties and soil microstructure. It was found that the soil evolution rates of debris flow waste-shoal land varied with different modes of reclamation. For the land which had been reclaimed for less than lO years, soil evolved most rapidly in paddy fields, and more rapidly in dry farmland than in naturally restored waste-shoal land. For the land which had been used for more than lo years, the soil evolution rates of dry farmland, naturally restored waste-shoal land and paddy farmland decreased in the file. For the same utilization period of time, significant differences were recognized in soil evolution features under different modes of reclamation. Analysis data showed that soil clay content, soil thickness, the psephicity of skeleton particles and contents of microaggregates （〈0.02 mm） in paddy farmland were all highest. Soil nutrients and porosity of dry farmland were better than those of paddy farmland and naturally restored waste-shoal land, and those of paddy farmland were superior to those of naturally restored waste-shoal land. Paddy farmland characterized by rapid pedogenesis, stable evolution and high utilizability was the priority candidate for the reclamation and utilization of debris flow waste -shoal land.     

RESPONSE OF EQUATORIAL PACIFIC UPPER OCEAN CURRENT TO WESTERLY WIND BURSTS

The characteristics of the response of equatorial Pacific upper ocean current to westerly wind bursts(WWB)were analyzed in the frequency domain by using wind and ADCP data collected by the Shiyan3 during TOGA-COARE IOP,1992-1993.The preliminary results showed that the response consistedof an eastward surface jet at shallower than 60m depth,a westward counter current centering near100m and a shear layer between them,with the variations of all three being nonlinear and nearlysynchronous.The oceanic responses in the frequency domain were characterized by occurrences of a remotely forced mixed Rossby-gravity wave with period of 8-10 days in the surface jet andcountercurrent at shallower than 110 m depth,and two locally forced waves with periods of 24 daysand 4-5 days limited in shallower than 70m depth.These fluctuations of the responses depended much more on zonal wind than meridional wind.The results also revealed that the oceanic response toWWB resulted from momentum transport and energy propagation assoc     

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赣西北地区P波S波随频率衰减关系研究

利用2009-01—2013-05月发生在赣西北地区的233次M L≥1.0地震数字波形资料,采用尾波归一化方法计算了阳新台、九江台、修水台、高安台、宜春台的P波S波的衰减,结果显示,除修水台最佳流逝时间约为60 s外,其他四个台站的最佳流逝时间为30 s左右;九江-瑞昌震区内平均P波和S波的Q值分别为:Q P(f)=(15.97±1.87)f(1.66±0.06)和Q S(f)=(25.27±1.90)f(1.15±0.04)。     

A calibration method of Argo floats based on multiple regression analysis

1 INTRODUCTION Argo floats are instruments that move freely with the ocean current at fixed parking depths and cycle from a profiling depth to the sea surface at regular time intervals. While rising to the surface, these autonomous floats take profiles of…     

Deriving Changjiang coastal zone wind from C-band SAR and its application to salinity simulation

Wind plays an important role in hydrodynamic processes such as the expansion of Changjiang （Yangtze） River Diluted Water （CDW）, and shelf circulation in the Changjiang estuary. Thus, it is essential to include wind in the numerical simulation of these phenomena. Synthetic aperture radar （SAR） with high resolution and wide spatial coverage is valuable for measuring spatially inhomogeneous ocean surface wind fields. We have collected 87 ERS-2 SAR images with wind-induced streaks that cover the Cbangjiang coastal area, to verify and improve the validity of wind direction retrieval using the 2D fast Fourier transform method. We then used these wind directions as inputs to derive SAR wind speeds using the C-band model. To demonstrate the applicability of the algorithms, we validated the SAR-retrieved wind fields using QuikSCAT measurements and the atmospheric Weather Research Forecasting model. In general, we found good agreement between the datasets, indicating the reliability and applicability of SAR- retrieved algorithms under different atmospheric conditions. We investigated the main error sources of this process, and conducted sensitivity analyses to estimate the wind speed errors caused by the effect of speckle, uncertainties in wind direction, and inaccuracies in the normalized radar cross section. Finally, we used the SAR-retrieved wind fields to simulate the salinity distribution off the Changjiang estuary. The findings of this study will be valuable for wind resource assessment and the development of future numerical ocean models based on SAR images.     

苏皖北部地区晚元古代地层的马尔柯夫链分析

本文运用数学地质理论中的马尔柯夫链分析方法,对苏皖北部地区及鲁西南地区晚元古代地层进行了初步研究。文中选择6条比较典型的剖面,分别对它们进行了转移概率、极限概率、差值阵、置换分析和熵分析等方面的计算,并对所得结果做了相应的地质解释。分析结果,从定量方面揭示出了晚元古代时期苏皖海盆基底的“波动”史及其上覆地层的主要沉积韵律类型,以及盆地内不同部位、不同时代岩性变化的主要趋势及其阶段性;并认为,在晚元古代早期苏皖海盆中很可能同时存在有南、北两个沉积中心,而并非像以往所认为的那样,只有一个沉积中心。