NEAR-FAULT DISPLACEMENT AND DEFORMATION OBTAINED FROM ONE-KILOMETER-LONG FAULT-CROSSING BASELINE MEASUREMENTS-A PRELIMINARY EXPERIMENT AT 2 SITES ON THE EASTERN BOUNDARY OF THE SICHUAN-YUNNAN BLOCK

The current and conventional fault-crossing short baseline measurement has a relatively high precision, but its measurement arrays usually fail to or cannot completely span major active fault zones due to the short length of the baselines, which are only tens to 100 meters. GNSS measurement has relatively low resolution on near-fault deformation and hence is not suitable for monitoring those faults with low motion and deformation rates, due to sparse stations and relatively low accuracy of the GNSS observation. We recently built up two experimental sites on the eastern boundary of the active Sichuan-Yunnan block, one crossing the Daqing section of the Zemuhe Fault and the other crossing the Longshu section of the Zhaotong Fault, aiming to test the measurement of near-fault motion and deformation by using fault-crossing arrays of one-kilometer-long baselines. In this paper, from a three-year-long data set we firstly introduce the selection of the sites and the methods of the measurement. We then calculate and analyze the near-field displacement and strain of the two sites by using three hypothetical models, the rigid body, elastic and composed models, proposed by previous researchers. In the rigid body model, we assume that an observed fault is located between two rigid blocks and the observed variances in baseline lengths result from the relative motion of the blocks. In the elastic model, we assume that a fault deforms uniformly within the fault zone over which a baseline array spans, and in the array baselines in different directions may play roles as strainmeters whose observations allow us to calculate three components of near-fault horizontal strain. In the composed model, we assume that both displacement and strain are accumulated within the fault zone that a baseline array spans, and both contribute to the observed variances in baseline lengths. Our results show that, from the rigid body model, variations in horizontal fault-parallel displacement component of the Zemuhe Fault at the Daqing site fluctuate within 3mm without obvious tendencies. The displacement variation in the fault-normal component keeps dropping in 2015 and 2016 with a cumulative decrease of 6mm, reflecting transverse horizontal compression, and it turns to rise slightly(suggesting extension)in 2017. From the elastic model, the variation in horizontal fault-normal strain component of the fault at Daqing shows mainly compression, with an annual variation close to 10^-5, and variations in the other two strain components are at the order of 10^-6. For the Longshu Fault, the rigid-body displacement of the fault varies totally within a few millimeters, but shows a dextral strike-slip tendency that is consistent with the fault motion known from geological investigation, and the observed dextral-slip rate is about 0.7mm/a on average. The fault-parallel strain component of the Longshu Fault is compressional within 2×10^-6, and the fault-normal strain component is mainly extensional. Restricted by the assumption of rigid-body model, we have to ignore homolateral deformation on either side of an observed fault and attribute such deformation to the fault displacement, resulting in an upper limit estimate of the fault displacement. The elastic model emphasizes more the deformation on an observed fault zone and may give us information about localizations of near-fault strain. The results of the two sites from the composed model suggest that it needs caution when using this model due to that big uncertainty would be introduced in solving relevant equations. Level surveying has also been carried out at the meantime at the two sites. The leveling series of the Daqing site fluctuates within 4mm and shows no tendency, meaning little vertical component of fault motion has been observed at this site; while, from the rigid-body model, the fault-normal motion shows transverse-horizontal compression of up to 6mm, indicating that the motion of the Zemuhe Fault at Daqing is dominantly horizontal. The leveling series of the Longshu site shows a variation with amplitude comparable with that observed from the baseline series here, suggesting a minor component of thrust faulting; while the baseline series of the same site do not present tendencies of fault-normal displacement. Since the steep-dip faults at the two sites are dominantly strike-slip in geological time scale, we ignore probable vertical movement temporarily. In addition, lengths of homolateral baselines on either side of the faults change somewhat over time, and this makes us consider the existence of minor faults on either side of the main faults. These probable minor faults may not reach to the surface and have not been identified through geological mapping; they might result in the observed variances in lengths of homolateral baselines, fortunately such variations are small relative to those in fault-crossing baselines. In summary, the fault-crossing measurement using arrays with one-kilometer-long baselines provides us information about near-fault movement and strain, and has a slightly higher resolution relative to current GNSS observation at similar time and space scales, and therefore this geodetic technology will be used until GNSS networks with dense near-fault stations are available in the future.     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

Impacts of salinity parameterizations on temperature simulation over and in a hypersaline lake

In this paper,we introduced parameterizations of the salinity effects(on heat capacity,thermal conductivity,freezing point and saturated vapor pressure) in a lake scheme integrated in the Weather Research and Forecasting model coupled with the Community Land Model(WRF-CLM). This was done to improve temperature simulation over and in a saline lake and to test the contributions of salinity effects on various water properties via sensitivity experiments. The modified lake scheme consists of the lake module in the CLM model,which is the land component of the WRF-CLM model. The Great Salt Lake(GSL) in the USA was selected as the study area. The simulation was performed from September 3,2001 to September 30,2002. Our results show that the modif ied WRF-CLM model that includes the lake scheme considering salinity effects can reasonably simulate temperature over and in the GSL. This model had much greater accuracy than neglecting salinity effects,particularly in a very cold event when that effect alters the freezing point. The salinity effect on saturated vapor pressure can reduce latent heat flux over the lake and make it slightly warmer. The salinity effect on heat capacity can also make lake temperature prone to changes. However,the salinity effect on thermal conductivity was found insignificant in our simulations.     

医疗设施布局的GIS优化评价——以翔安区医疗设施为例

开展现有医疗设施布局的分析与评价,对解决公众医疗需求与医疗服务供给的矛盾,以及政府部门决策具有重要意义。本文在厦门市第一次全国地理国情普查试点的背景下,以翔安区医疗设施为研究对象,在地理国情普查数据结果的基础上,结合卫生等专业部门的统计数据,从医疗设施的现状、服务水平和空间布局优化3个角度,构建了医疗设施布局的评价指标体系。从医疗卫生角度分析医疗资源现状特征;基于GIS网络分析开展医疗设施服务水平的分析与评价;利用GIS"位置分配模型"对医疗设施空间布局进行优化。最后,本文对医疗设施布局提出改善建议和措施。研究结果表明:(1)翔安区医疗资源总量不足,分布不均衡;(2)城乡医疗设施差异明显,医疗服务水平不高;(3)翔安区需新增4家医院,5家卫生院才能满足居民就医需求。本研究可以为今后翔安区医疗设施布局决策提供借鉴和参考,为医疗设施布局评价提供一个可行的方法。     

盐度调控对花鲈(Lateolabrax maculatus)生长相关基因表达的影响

生长激素受体(GHR)作为GH/IGF轴的中心环节,在内分泌调控中发挥重要作用。本实验采用c DNA末端快速扩增法(RACE)技术克隆出花鲈GHR1和GHR2的c DNA全长序列。急性低盐度调控实验设为海水组、半海水组和淡水组。测定了急性低盐度调控24、48、96、144、192h后,花鲈肝脏中GHRs、IGF-1及垂体GH表达情况。结果表明,GHR1 c DNA全长序列2436bp,编码637个氨基酸;GHR2 c DNA全长序列2940bp,编码582个氨基酸。GHR1与GHR2由信号肽、胞外区、跨膜区、胞内区组成,且结构存在差异。脑、肾、鳃中GHR1表达明显高于GHR2;而在肌肉、垂体、肝脏、盲肠、胸腺、心脏中,GHR2表达明显高于GHR1。24h时,各组GHR1表达不变,GHR2、GH、IGF-1显著下降。之后,相对于海水组,淡水组和半海水组GHRs和IGF-1表达升高,而GH下降,GH与GHR负相关。据结果推测,花鲈GHR2可能为SL受体,GH/IGF轴参与低渗调控可能是通过增加GHRs,进而激活下游IGF-1表达而实现。     

便携式高频地波雷达台湾海峡浪高观测

As an important equipment for sea state remote sensing, high frequency surface wave radar(HFSWR) has received more and more attention. The conventional method for wave height inversion is based on the ratio of the integration of the second-order spectral continuum to that of the first-order region, where the strong external noise and the incorrect delineation of the first- and second-order Doppler spectral regions due to spectral aliasing are two major sources of errors in the wave height. To account for these factors, two more indices are introduced to the wave height estimation, i.e., the ratio of the maximum power of the second-order continuum to that of the Bragg spectral region(RSCB) and the ratio of the power of the second harmonic peak to that of the Bragg peak(RSHB). Both indices also have a strong correlation with the underlying wave height. On the basis of all these indices an empirical model is proposed to estimate the wave height. This method has been used in a three-months long experiment of the ocean state measuring and analyzing radar, type S(OSMAR-S), which is a portable HFSWR with compact cross-loop/monopole receive antennas developed by Wuhan University since 2006. During the experiment in the Taiwan Strait, the significant wave height varied from 0 to 5 m. The significant wave heights estimated by the OSMAR-S correlate well with the data provided by the Oceanweather Inc. for comparison, with a correlation coefficient of 0.74 and a root mean square error(RMSE) of 0.77 m. The proposed method has made an effective improvement to the wave height estimation and thus a further step toward operational use of the OSMAR-S in the wave height extraction.     

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鲤JAK2基因分子克隆及组织表达分析

采用同源克隆策略和RACE-PCR技术,克隆得到可能的鲤(Cyprinus carpio)两面神激酶2(JAK2)基因的c DNA全长序列,包括3 378 bp的开放阅读框,732 bp的5'-非编码区,529 bp的3'-非编码区,总长度达4 639bp。开放阅读框可编码1 125个氨基酸,推测分子质量和理论等电点分别为129.33 ku和6.99。同源性分析显示,克隆的鲤鱼基因与斑马鱼(Danio rerio)JAK2同源性最高,其氨基酸序列同一性和相似度分别达89%和95%。结构域预测表明,所编码氨基酸序列包含FERM、SH2及两个酪氨酸激酶结构域,这4个结构域也保守地存在于其他脊椎动物的JAK分子中。高级结构预测显示,鲤JAK2主要包括α-螺旋(α-helix)、β-折叠(β-sheet)和连接环(loop)等3类结构元件。脊椎动物JAK分子系统进化树显示,JAK1、JAK2、JAK3和TYK2等4类JAK分子分别聚类,鲤JAK2处于JAK2支系中,与所有其他的鱼类JAK2聚为一大支,并与两栖类和哺乳类组成的另一大支构成姊妹群,表明它们具有共同的祖先基因,为直系同源关系。实时荧光定量PCR检测结果表明,鲤JAK2在皮肤中表达量最高,其次是肠、血液和脑,而在肌肉、鳃、头肾、心、肝、脾中表达量较低;鲤JAK3在脾中表达量最高,在其他组织中表达量均很低,甚至未检出。     

滇东南老君山矿集区北部长田W(Sn)矿床成矿时代：来自白钨矿和萤石 Sm- Nd同位素定年的制约

滇东南是我国重要的钨锡多金属成矿区,老君山矿集区是其重要组成部分,围绕燕山期老君山复式花岗岩体,分布有都龙、南秧田、新寨大型-超大型Sn-W多金属矿床及多个中小型矿床(点)。该区地质演化复杂,Sn、W多金属可能存在多期成矿作用,包括燕山期、印支期和加里东期。由于目前该区发现的Sn-W矿化多分布于老君山岩体南、东侧,而岩体北部Sn-W成矿作用的研究相对较薄弱,特别是成矿年代学方面,多数矿床依然缺少精确同位素年代学数据,制约了该区Sn-W多金属成矿作用的认识和地质勘探的深入。长田是老君山岩体北缘代表性W (Sn)多金属矿床,区内加里东期和燕山期花岗岩均有分布,由于缺少年代学数据和地质地球化学研究,W(Sn)成矿作用的认识尚缺少实际地质地球化学依据,难以建立合理的成矿模式,制约了区内地质勘探的深入和突破。基于此,本文通过对长田W (Sn)矿床中白钨矿及共生萤石的Sm-Nd同位素定年,分别获得97 Ma和79 Ma两个年代学数据,显示该矿床钨矿化主成矿期应为燕山晚期,与老君山花岗岩活动时限一致,表明长田钨成矿作用与燕山期老君山花岗岩的侵入活动关系密切,而与加里东期岩浆活动关系不大。该成果丰富了...     

对陡坡陡崖引发地质灾害的潜在影响范围进行建模分析

陡坡陡崖作为一种特殊的地形地貌,是造成滑坡、泥石流等各种地质灾害的重要因素之一。由陡坡陡崖引发的地质灾害会破坏人造地物,对人类的生命财产安全造成严重危害。本文利用DEM数据提取了浙江省温州市的陡坡陡崖,对温州市陡坡陡崖的影响范围进行系统研究,构建了温州市陡坡陡崖的潜在滑坡方向模型,并确定其对人造地物（如道路、房屋）的影响。本文主要研究内容有以下两个：①提取陡坡陡崖并对其潜在滑坡方向进行建模;②根据影响范围提取受影响的人造地物并建立基础数据库。结果表明,温州市的陡坡陡崖数量多,分布范围广且集中,其引发的地质灾害会对人造地物造成严重的潜在影响。本文构建的温州市基础数据库可以描述陡坡陡崖潜在影响的房屋和道路的位置,确定灾害重点防治区域,为温州市的灾害防治工作提供服务。