Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

ASM1中污水水质参数与化学计量系数的估测

简要介绍了活性污泥1号模型中14个污水水质参数与5个化学计量系数的估测方法和典型取值。     

饵料对中间球海胆生长发育的影响初探

实验结果表明 :单独投喂牟氏角毛藻或与三角褐指藻、绿色巴夫藻混投 ,中间球海胆幼虫成活率最高 ,其生长速度也最快 ,中间球海胆稚幼海胆培育的最好饵料是前期投喂的石莼等薄嫩的海藻 ,后期投喂海带其生长速度最快     

钦州湾内湾贝类养殖海区水环境特征及营养状况初探

根据 1 998年 1 0月和 1 999年 5月的调查资料 ,阐述了钦州湾内湾水环境特征及 N/P的变化 ,以 DIN、DIP、COD和 Chl- a为富营养化指标 ,用营养状态质量指数 (NQI )分析探讨了该湾的营养状态 ,并从富营养角度讨论了该湾的污染程度     

Vertical Sound Velocity Transition in the Coral Reef Core and Its Significance of Indicating Facies

On the basis of the sound velocity measurements of the coral reef core from Nanyong No.1 well of Yongshu Reef in the Nansha Islands,the paper studies the relations between the vertical sound velocity transition features in the coral reef core and the corresponding stratigraphic depositional facies change as well as stratigraphic gap of erosion,analyses the cause of the sound velocity transition,expounds the concrete process of the sea level change resulting in the stratigraphic gap of erosion and facies change in the coral reef and explains the relations between the vertical sound velocity transition in the coral reef core and the corresponding stratigraphic paleoclimate and the sea level change.This study is of important practical value and theoretical significance to the island and reef engineering construction and the acoustic logging for oil exploration in the reef limestone area as well as the paleoceanographic study of the marginal sea in the westerm Pacific Ocean.     

白令海夏季浮游细菌和原生动物生物量及分布特征

1999年7月21日至8月1日在我国首次北极科学考察期间,考察了白令海中部的浮游细菌和原生动物,分析了其丰度、分布、生物量及其生态作用,结果显示,浮游细菌表层生物量为1.5～20.2μg/dm3,平均为浮游植物生物量的30%,100m以上水柱柱总生物量(720～3123mg/m2)平均为浮游植物柱总生物量的67%,因而是白令海夏季与浮游植物处同等量级的优势类群;原生动物表层生物量为1.2～27.4μg/dm3,100m以上水柱柱总生物量为189～1698mg/m2,平均为浮游植物柱总生物量的21%,其中粒径小于5,5～20μm和大于20μm的原生动物分别占其柱总生物量的13%,47%和40%;作为主要类群的异养腰鞭毛虫占原生动物柱总生物量的39%.浮游细菌和原生动物生物量的总体分布趋势从西部向东北和东部递减、从表层向深层衰减,20～25m水层温跃层和表层海流的存在对这一分布特性可能有较大的影响.原生动物受潜在的大、中型浮游动物捕食压力的制约,维持了一个相对较低的生物量水平,在一定程度上限制了微食物环(microbial food loop)在该海域夏季生态系统营养中的作用.     

一类全局最优化问题的动态隧道方法

研究了连续变量函数的全局最优化问题 ,给出了动态隧道方法。该动态隧道方法由局部搜索和动态隧道 2个阶段构成。在局部搜索阶段用了动态系统方法。对全局最优化问题的实例进行了数值实验 ,数值结果表明了该方法的稳健性和有效性。     

Seasonal dynamics of elemental sulfur in two coastal sediments

A spectrophotometric method for elemental sulfur (S⁰) analysis without interference from other reduced sulfur compounds was adapted for the use in reducing sediments. The S⁰ distribution in two coastal sediments was studied regularly from summer to winter and compared to factors regulating the S⁰ accumulation, such as redox potentials, the rate of bacterial sulfide production and the general sulfur chemistry. Dense coatings of sulfur bacteria developed on the sediment surface of a sulfuretum which had an S⁰ concentration of up to 41 μmol S cm^?3. The 2·5-mm thick bacterial coating contained 40% of all S⁰ in the sediment. A more typical marine sediment with a few cm thick oxidized surface layer had an S⁰ maximum of 1–3 μmol S cm^?3 at 2–4 cm depth. The S⁰ maximum in both sediments increased from summer to winter as the sediments gradually became more oxidized. The deeper layers maintained a low S⁰ concentration. Most of the S⁰ in the upper few mm of a laboratory sulfuretum was present inside sulfur bacteria and actively migrated up and down with the bacteria depending upon the changing light and oxygen conditions.     

对虾封闭式工厂化养殖水环境要素的变化特征及其相互关系

根据2002年8月7～8日对虾工厂化养成中后期A、B代表池的实测数据，分析讨论了该时期养殖水体中环境要素的变化特征及其相互关系．结果表明：研究水体水温、盐度和溶解氧含量适宜．日变化幅度不大．但pH值略为偏低，而COD值明显偏高．与该时期投饵量大，残饵及排泄物明显增加有关．但对A池来说，生物作用的影响占主导地位，而对B池而言．则以化学过程影响为主．     

杰氏风暴潮预报方法在珠海及粤西海域的应用

本文通过杰氏风暴潮预报方法对历史上给珠海和粤西海域造成较大影响的台风增水事件进行后报试验,并通过统计分析方法对原有的预报公式进行订正,总结出适合珠海及粤西海域的风暴潮预报公式.利用5a的时间对珠海及粤西海域的台风增水进行试报,结果表明预报准确率分别达95.0%和87.6%,尤其对一些增水较大、影响较严重的台风增水预报相对更加准确,预报准确率均达90.0%以上.这说明订正后的公式对珠海及粤西海域的风暴潮预报能得到较好的预报效果.