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101.
Isaac Wirgin Blake Konkle Mark Pedersen Cheryl Grunwald Jim Williams Simon C. Courtenay 《Estuaries and Coasts》1996,19(4):913-922
Quantification of levels of cytochrome P4501A (CYP1A) gene expression in sentinel species of fishes has been proposed as a management tool to evaluate contamination of aquatic systems. Based on preliminary studies, we hypothesized that differences in CYP1A mRNA inducibility among individuals, populations, or species might lead to spurious conclusions when using this approach in environmental monitoring programs. To address this possibility, we quantitated and compared CYP1A mRNA induction levels in four species of common Atlantic Coast estuarine fish: smooth flounder, hogchoker, striped bass, and Atlantic tomcod, which were treated with model chemicals (beta naphthoflavone (β-NF), or benzo[a]pyrene at 10 ppm) known to induce CYP1A mRNA, or were exposed to contaminated environments. Species-specific CYP1A DNA probes were generated from PCR (polymerase chain reaction) amplification of genomic DNA using conserved oligonucleotide primers, and, along with cloned rainbow trout and Atlantic tomcod CYP1A cDNA probes were used to quantify CYP1A mRNA levels in northern blot analyses. Successful PCR amplification of CYP1A hybridizable DNA fragments was observed for all four species. Results from northern blot analyses showed large differences in CYP1A mRNA induction among species; only Atlantic tomcod exhibited significant induction of CYP1A mRNA for both chemically treated (97-fold) and environmentally exposed fish (34-fold). Significant, although lower, levels of induction were observed in β-NF treated (14-fold) smooth flounder, but not in environmentally exposed smooth flounder. Only low levels (not significant) of CYP1A gene induction were detected in hogchokers and striped bass. We conclude that CYP1A mRNA inducibility differed significantly among fish taxa perhaps due to differences in regulation of gene expression, suggesting that careful selection of sentinel species should be exercised prior to the use of CYP1A mRNA induction in environmental monitoring programs. However, the significance of differences in CYP1A mRNA inducibility in relation to higher level biological endpoints has yet to be determined. 相似文献
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L. A. J. Williams 《Bulletin of Volcanology》1970,34(2):439-465
The paper reviews the stratigraphy, style of activity and some aspects of the petrology of Tertiary to Recent sodic alkaline volcanic rocks in Kenya, eastern Uganda and northern Tanzania. Repeated extrusions of basaltic and nephelinitic volcanics occurred from Miocene times onwards, confirming indications from chemical data that magmas of these compositions were parental. At some central volcanoes, a basalt-trachyte-phonolite series evidently arose by fractional crystallization of basaltic magma, whereas various courses of crystallization from a nephelinitic parent led to the production of phonolites, tephrites and basanites as well as olivine-and melilite-bearing nephelinites and melanephelinites. Phonolitic and trachytic volcanics which dominate an area of repeated upwarping (the Kenya dome) probably originated by processes of partial melting rather than by differentiation of basaltic magma. The basalt-trachyte association which characterizes many central volcanoes north and south of the dome can perhaps best be explained by postulating independent sources for the basic and salic volcanics. 相似文献
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Distance from auroral zone is a fundamental parameter in studies of disturbances produced in the thermosphere and ionosphere through the action of the solar wind. Calculations showing the great variation of the distances of the auroral “zones” from the magnetic equator and geographic equator are presented in diagrams. An auroral zone proximity index is proposed for use in correlative studies of upper atmosphere and of ionospheric disturbances. 相似文献