大姚6级双震前的地震前兆特征

在连续形变观测资料中除了存在于整个时间域的短周期潮汐信息(固体潮)外，还有出现在某个时间段的长周期非潮汐信息．如何从时间或空间上认识和提取这些信息．成为认识地震过程和进行地震预报关键和迫切的问题之一。利用小波分析的时一频特性和奇异性检测特性，对大姚6级双震前的连续形变观测资料进行了处理，提取潮汐资料中的非潮汐信息。发现在地震前震中附近的形变台站都接收到了相同频段的异常信号．周期为几天到十几天，出现在各个台站的时间也大致相同。这些在震前具有一定时、空分布的信息有可能是地震前兆。     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

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Combined effects of light intensity and NH^＋4-enrichment on growth,pigmentation, and photosynthetic performance of Ulva prolifera （Chlorophyta）

The aim of this study was to investigate the effects of light intensity and enhanced nitrogen supply on the growth and photosynthesis of the green-tide macroalga, Ulva prolifera. Thalli of U. prolifera were grown in natural or NH 4 +-enriched seawater under two different light intensities for 7 days, and then the growth rate, pigmentation, and photosynthetic performance of the thalli were evaluated. The results show that the relative growth rate(RGR) was markedly higher under the high light level than under the low light level. Enrichment with NH 4 + enhanced the RGR under high light intensity, but did not affect RGR under low light intensity. In low light conditions, NH 4 +-enrichment resulted in a marked decrease in the maximal photosynthetic rate( P m) and the maximum carbon fixation rate( V max), but it did not affect the half saturation constant for carbon( K 0.5) or the ratio of V max to K 0.5, which reflects the carbon acquisition efficiency. In high light conditions, P m, K 0.5, and the dark respiration rate( R d) increased under NH 4 + enrichment, but V max and the V max / K 0.5 ratio decreased. Regardless of the light intensity, NH 4 +-enrichment did not affect the apparent photosynthetic efficiency( α), which refl ects the ability of the alga to use light energy at low light levels. Under both low and high light intensities, the chlorophyll a(Chl a), chlorophyll b(Chl b), and carotenoids(Car) contents in thalli were higher in NH 4 +-enriched than in natural seawater, except that there was a decrease in the Chl b content of thalli in NH 4 +-enriched seawater under low light intensity. Therefore, NH 4 + enrichment improved the growth and photosynthetic performance of U. prolifera under high light intensity, but not under low light intensity. We discuss the possible mechanisms underlying these physiological responses.     

NUMERICAL SIMULATION OF INTERANNUAL AND INTERDECADAL VARIABILITY OF SURFACE WIND OVER THE TROPICAL PACIFIC

A global atmospheric general circulation model (L9R15 AGCMs) forced by COADS SST was integrated from 1945 to 1993. Interannual and interdecadal variability of the simulated surface wind over the tropical Pacific was analyzed and shown to agree vey well with observation. Simulation of surface wind over the central-western equatorial Pacific was more successful than that over the eastern Pacific. Zonal propagating feature of interannual variability of the tropical Pacific wind anomalies and its decadal difference were also simulated successfully. The close agreement between simulation and observation on the existence of obvious interdecadal variability of tropical Pacific surface wind attested to the high simulation capability of AGCM.     

VERTICAL PROFII,ES OF CHL-A AND PRIMARY PRODUCTIVITY IN THE MIDDLE CONTINENTAL SHELF AREA AND EDDY AREA OF THE EAST CHINA SEA

Vertical profiles of chl-a and primary productivity in the middle continental shelf area and eddy area of the East China Sea were studied using data from a cruise in the East China Sea in February to March, 1997 and a cruise in July, 1998. The results showed that chl-a vertical distribution closely related to in situ hydrological and nutrient conditions. Chla-a concentration ranged from 0.22 to 0.35 mg/m³ and 0.93–1.09 mg/m³ in the eddy area and in the middle continental shelf area, respectively. In both areas, chl-a concentrations in deep layers were slightly higher than those in shallow layers, but was of the same order of magnitude. In summer, when a thermocline existed in the water column, highest chl-a concentrations appeared at the base of the thermocline layers in both areas. In the eddy area, chl-a concentration maximized at 31.743 mg/m³, and averaged 1.143 mg/m³ below 30 m depth. In the middle continental shelf area, the highest chl-a concentration was 2.120 mg/m³, the average was 1.168 mg/m³. The primary productivity reached 1418.76 mgC/(m²·d) in summer and 1360.69 mgC/(m²·d) in winter. In the eddy area, the primary productivity was 787.50 mgC/(m²·d) in summer and 159.04 mgC/(m²·d) in winter. Vertical carbon sinking rate from the deep layer to the bottom in both areas is also discussed in this paper. Contribution NO. 4183 from the Institute of Oceanology, Chinese Academy of Sciences. Project No. 49636210 supported by NSFC.     

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226Ra and 228Ra in the seawater of the western Yellow Sea

1Introduction Therearefourradiumisotopesinnature,ofwhich224Ra,226Raand228Rahavebeenwidelyinvestigated inmarineresearch,butthespecificactivityof223Rais toolowtobemeasured,therefore,itsapplicationsarelimited(Moore,1969;Broeckeretal.,1976;KuandLin,1976;Nozak…     

基于大数据的城市地质环境智能监管思路与方法

随着城市化进程加速,城市范围迅猛扩大,人口急剧膨胀,高层建筑林立,地下空间开发规模化,面临着水资源贫化、地质环境恶化和地质灾害频发等巨大挑战.为了有效地应对挑战,迫切需要在城市多要素地质环境调查并建立完善的信息系统的基础上,开展面向“智慧城市”的精细、全息三维地质建模,即建立新型的城市“玻璃国土”,实现城市地质环境时空透视,然后利用传感器、物联网和云技术,建立城市地质资源、地质环境和地质灾害动态监测数据链,最后基于地质科学大数据的同化、融合和挖掘技术,进行智能预警和管控.