Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy

Here, we describe a technique that allows the genetic linage analysis of 16S rRNA genes in bacteria observed under a microscope. The technique includes the isolation of microbial cells using a laser microdissection microscope, lysis of the cells, and amplification of the 16S rRNA genes in the isolated cells without interference by bacterial DNA contamination from the experimental environment or reagents. Using this technique, we successfully determined 15 16S rRNA gene sequences in cells isolated from an Antarctic iceberg. These sequences showed 94%–100% identity to their closest strains, which included bacteria that occur in aqueous, marine, and soil environments.     

Street-scale storm surge load impact assessment using fine-resolution numerical modelling: a case study from Nemuro,Japan

Natural Hazards - Due to gradual sea level rise and changes in the climate system, coastal vulnerability to storm surge hazards is expected to increase in some areas. Studies regarding the effect...     

High-frequency ocean radar derived characteristics of sea surface currents in the Ariake Sea,Japan

Composite analysis was conducted using high-frequency radar data obtained during 2006–2015 in order to gain a better understanding of the current field in the Ariake Sea. The seasonally averaged surface current in the Ariake Sea was directed southward in all seasons, except around river mouths during summer. Heavy rainfall enhanced the outflow along the eastern coast of the Shimabara Peninsula from Isahaya Bay to the southern area 2–5 days after heavy rainfall. Spring–neap differences were clearly seen in the southward current along the Shimabara Peninsula. Interannual variation in the M₂ tidal current amplitude was synchronized with the lunar nodal cycle.     

GEK measurements in a shallow water region

Evidence for the applicability of GEK (Geomagnetic Electrokinetography) measurements to shallow water regions is provided from observations in the shelf region of the East China Sea. The reason for the effectiveness of GEK measurements in this case is investigated theoretically, and it is shown to be attributable to the existence of a thick conductive sedimentary layer. In addition, it is shown that low conductive basement rock can be regarded as a good conductor for GEK measurements if the current width is broad enough and if the ratio of current width to water depth is larger than the resistivity ratio of basement rock to sea water. This implies that barotropic tidal currents can be measured with GEK in any ocean on the earth if they have significant magnitudes.     

Stage-V copepodites of Calanus sinicus and Calanus jashnovi (Copepoda: Calanoida) in mesopelagic zone of Sagami Bay as identified with genetic markers, with special reference to their vertical distribution

We applied genetic makers to identify Calanus species occurring in Sagami Bay, Japan, in order to investigate their vertical distribution in the upper 1000 m. First, interspecific genetic distances of three gene loci, mitochondrial small ribosomal RNA (srRNA), nuclear internal transcribed spacers 1 (ITS1) and 2 (ITS2), were estimated from morphologically distinguishable adult females of Calanus sinicus, Calanus jashnovi and Calanus pacificus that were collected from Sagami Bay, the Kuroshio Extension and the Oyashio region, respectively. The highest levels of interspecific genetic distance were observed in srRNA, followed by ITS1 and ITS2. The intraspecific genetic distances within C. sinicus were much lower than the interspecific genetic distances, indicating that DNA sequences in these loci are consistent with the morphological differences. This information was used as a criterion for species identification based on DNA sequence variation, and allowed us to identify the fifth copepodites (CVs) or younger stages of these species. Next, the vertical distribution of Calanus species was investigated in Sagami Bay in May 2006, on the basis of a stratified sampling in the upper 1000 m. By applying the genetic markers, 23 individuals comprising all copepodite stages were allocated into either C. sinicus or C. jashnovi, and the small- and large-sized CVs were identified as C. sinicus and C. jashnovi, respectively. The total abundance of C. sinicus was highest at 0-50 m and decreased with depth. On the contrary, CV individuals of C. sinicus were abundant not only in 0-50 m but also below 200 m with minimum occurrences in 150-200 m depth. C. jashnovi was much less abundant than C. sinicus and comprised of only CIV and CV which occurred in the upper 100 m and deeper than 50 m depths, respectively. The abundance of C. sinicus in the 1000-m water column of Sagami Bay was at a level comparable to that in shelf waters, suggesting the importance of off-shelf individuals in the biological production and organic transport in the respective areas.     

Magnetotail response to sudden changes in the interplanetary magnetic field

The effect of southward or northward changes in the interplanetary magnetic field is examined statistically in the nightside magnetosphere over the range of 6.6 to 80R _E from the Earth. After southward changes, the deformation of the magnetosphere toward a greater antisunward extension of field lines occurs at 6.6R _E with 10 min delay and spreads down the tail to 80R _E in 30 min. Around the onset of the field-line collapse that occurs 1–2 hr later, the southwarddirected field is observed briefly in the distant tail. The effect of northward changes could not be recognized in the lobe region of the tail.     

The main sequence stars and the photon-neutrino coupling theory of weak interactions

The tetrad field equations of general relativity discussed in previous articles are applied to Robertson-Walker cosmological models. A generalized Friedmann equation is derived and some of its consequences are discussed.     

Tsunami vulnerability evaluation in the Mentawai islands based on the field survey of the 2010 tsunami

On October 25, 2010, a large earthquake occurred off the coast of the Mentawai islands in Indonesia, generating a tsunami that caused damage to the coastal area of North Pagai, South Pagai, and Sipora islands. Field surveys were conducted soon after the event by several international survey teams, including the authors’. These surveys clarified the tsunami height distribution, the damage that took place, and residents’ awareness of tsunamis in the affected islands. Heights of over 5 m were recorded on the coastal area of the Indian Ocean side of North and South Pagai islands and the south part of Sipora island. In some villages, it was difficult to evacuate immediately after the earthquake because of the lack of routes to higher ground or the presence of rivers. Residents in some villages had taken part in tsunami drills or education; however, not all villages shared awareness of tsunami threats. In the present paper, based on the results of these field surveys, the vulnerability of these islands with regards to future tsunami threats was analyzed. Three important aspects of this tsunami disaster, namely the geographic disadvantage of the islands, the resilience of buildings and other infrastructure, and people’s awareness of tsunamis, are discussed in detail, and corresponding tsunami mitigation strategies are explained.