Relevance of Pentane- and Hexane-Utilizing Bacterial Indicators for Finding Hydrocarbon Microseepage: A Study from Jamnagar Sub-basin, Saurashtra, Gujarat, India

Mesozoic sediments are source rocks for nearly half the world’s hydrocarbon reserves. Hence, there is great interest in the oil industry to know the trap and sub-trappean sediment thickness and their extent in the trap covered regions of Jamnagar study area. The microbial prospecting method is applied in the Jamnagar sub-basin, Gujarat for evaluating the prospects for hydrocarbon exploration by investigating the anomalous abundance of n-pentane- and n-hexane-oxidizing bacteria of this area. A total of 150 near-surface soil samples were collected in Jamnagar sub-basin, Gujarat for the evaluation of hydrocarbon resource potential of the basin. In this study, bacterial counts for n-pentane-utilizing bacteria range between 1.09 × 10² and 9.89 × 10⁵ cfu/g and n-hexane-utilizing bacteria range between 1.09 × 10² and 9.29 × 10⁵ cfu/g. The adsorbed hydrocarbon gases consisting of ethane plus hydrocarbons (ΣC₂₊) of 1–977 ppb and n-pentane (nC₅) of 1–23 ppb. The integrated geomicrobial and adsorbed soil gas studies showed the anomalous hydrocarbon zones nearby Khandera, Haripur, and Laloi areas which could probably aid to assess the true potential of the basin. Integrated geophysical studies have shown that Jamnagar sub-basin of Saurashtra has significant sediment thickness below the Deccan Traps and can be considered for future hydrocarbon exploration.     

Northeast monsoon rainfall variability over south peninsular India and its teleconnections

Rainfall over south peninsular India during the northeast (NE) monsoon season (Oct–Dec) shows significant interannual variation. In the present study, we relate the northeast monsoon rainfall (NEMR) over south peninsular India with the major oscillations like El Ni?o Southern Oscillation (ENSO), Indian Ocean Dipole (IOD), and Equatorial Indian Ocean Oscillation (EQUINOO) in the Indian and Pacific Oceans. For establishing the teleconnections, sea surface temperature, outgoing long wave radiation, and circulation data have been used. The present study reveals that the positive phase of ENSO, IOD, and EQUINOO favor the NEMR to be normal or above normal over southern peninsular India. The study reveals that the variability of NEMR over south peninsula can be well explained by its relationship with positive phase of ENSO, IOD, and EQUINOO.     

Exact Bianchi type-II, VIII and IX perfect fluid cosmological models in Saez-Ballester theory of gravitation

Exact Bianchi type-II, VIII and IX cosmological models are obtained in a scalar tensor theory proposed by Saez and Ballester (Phys. Lett. A 113:467, 1986) with perfect fluid as a source. Some physical and geometrical properties of the models are studied. It is observed that the models are free from initial singularities and they are expanding with time.     

Chemical,isotopic and amino acid composition of Mukundpura CM2.0(CM1) chondrite: Evidence of parent body aqueous alteration

The carbonaceous chondrites are intriguing and unique in the sense that they are the only rocks that provide pristine records of the early solar nebular processes. We report here results of a detailed mineralogical, chemical, amino acid and isotopic studies of a recently observed fall at Mukundpura, near Jaipur in Rajasthan, India. Abundance of olivines in this meteorite is low and of serpentine minerals is high. FeO/SiO_2 = 1.05 in its Poorly Characterized Phases(PCP) is similar to that observed in other CM2.0 chondrites. The water content of ～9.8 wt.% is similar to that found in many other CM chondrites.Microscopic examination of matrix shows that its terrestrial weathering grade is WO but aqueous parent body alteration is high, as reflected in low abundance of identifiable chondrules and abundant remnants of chondrules(～7%). Thus, most of the chondrules formed initially have been significantly altered or dissolved by aqueous alterations on their parent bodies. The measured bulk carbon(2.3%) and nitrogen content and their isotopic(δ¹³C =-5.5‰, δ¹⁵N = 23.6%0) composition is consistent with CM2.0 classification probably bordering CM1. Several amino acids such as Alanine, Serine, Proline, Valine, Threonine,Leucine, Isoleucine, Asparagine and Histamine are present. Tyrosine and Tryptophan may occur in trace amounts which could not be precisely determined. All these data show that Mukundpura chondrite lies at the boundary of CM2.0 and CM1 type carbonaceous chondrites making it one of the most primitive chondrites.     

Characterization of magnetite particles in shocked quartz by means of electron- and magnetic force microscopy: Vredefort, South Africa

Submicroscopic opaque particles from highly shocked granite-gneisses close to the core of the Vredefort impact structure have been investigated by means of micro-analytical techniques with high spatial resolution such as electron diffraction, orientation contrast imagery and magnetic force microscopy. The opaque particles have been identified as nano- to micro-sized magnetite that occur in several distinct modes. In one sample magnetite occurs along relict planar deformation features (PDFs) in quartz, generally accepted as typical shock lamellae. The magnetite particles along shock lamellae in quartz grains virtually all show uniform crystallographic orientations. In most instances, the groups of magnetite within different quartz grains are systematically misorientated such that they share a subparallel <101> direction. The magnetite groups of all measured quartz grains thus appear to have a crystallographic preferred orientation in space. In a second sample, orientations of magnetite particles have been measured in microfractures (non-diagnostic of shock) of quartz, albite and in the alteration halos, (e.g. biotite grains breaking down to chlorite). The crystallographic orientations of magnetite particles are diverse, with only a minor portion having a preferred orientation. Scanning electron microscopy shows that magnetite along the relict PDFs is invariably associated with other microcrystalline phases such as quartz, K-feldspar and biotite. Petrographic observations suggest that these microcrystalline phases crystallized from locally formed micro-melts that intruded zones of weakness such as microfractures and PDFs shortly after the shock event. The extremely narrow widths of the PDFs suggest that heat may have dissipated rapidly resulting in melts crystallizing relatively close to where they were generated. Magnetic force microscopy confirms the presence of magnetic particles along PDFs. The smallest particles, <5 μm with high aspect ratios 15:1 usually exhibit intense, uniform magnetic signals characteristic of single-domain magnetite. Consistent offsets between attractive and repulsive magnetic signals of individual single-domain particles suggest consistent directions of magnetization for a large proportion of particles. Received: 16 November 1998 / Accepted: 17 May 1999     

Studies on some properties of coronal mass ejections based on angular width

The paper investigates the effects of thermal conductivity and non-uniform magnetic field on the gravitational instability of a non-uniformly rotating infinitely extending axisymmetric cylinder in a homogeneous heat conducting medium. The non-uniform rotation and magnetic field are supposed to act along θ and z directions of the cylinder. It is found that the gravitational instability of this general problem is determined by the same criterion as obtained by Dhiman and Dadwal (Astrophys. Space Sci. 325(2):195–200, 2010) for the self-gravitating isothermal medium in the presence of non-uniform rotation and magnetic field with the only difference that adiabatic sound velocity is now replaced by the isothermal sound velocity. It is found that the thermal conductivity has stabilizing effect on the onset of gravitational instability. Further, the stabilizing/destabilizing effect of the non-uniform magnetic field on the gravitational instability of heat conducting medium has been discussed and is illustrated by considering some special forms of the basic magnetic fields.     

Bioremediation of Phenanthrene by Mycoplana sp. MVMB2 Isolated from Contaminated Soil

The effect of nutrient and surfactant addition on the biodegradation of phenanthrene was studied in a batch scale soil–slurry system using isolated Mycoplana sp. MVMB2strain. The study was conducted using an artificially phenanthrene spiked and as well as contaminated soil from petrochemical industrial site. Maximum phenanthrene degradation and subsequent high microbial growth were observed at optimum pH (pH 6) and C/N/P ratio (100:20:3). To investigate maximum substrate degradation potential of Mycoplana sp. MVMB2, very high concentrations of phenanthrene (50–200 mg/kg soil) were used. The organism was capable of degrading >60% for a concentration below 20 mg/kg soil and >40% for concentrations up to 200 mg/kg within 8 days. Further the influence of five different surfactants namely Span 80, Tween 20, Triton X‐100, cetyl trimethyl ammonium bromide, and sodium dodecyl sulfate were tested at their critical micelle concentration (CMC) levels for phenanthrene degradation in the soil. The addition of surfactant enhanced the biodegradation and a maximum of 84.49% was obtained for Triton X‐100. Complete phenanthrene degradation by Mycoplana sp. MVMB2 was observed at 3 CMC concentration of Triton X‐100. The optimized parameters obtained were used for the degradation of phenanthrene present in the contaminated soil and 98.6% biodegradation was obtained. Thus, the results obtained in the study suggested that biodegradation of phenanthrene by Mycoplana sp. MVMB2 appeared to be feasible to remediate phenanthrene rich contaminated sites.     

Evaluation of fuzzy-based classifiers for cotton crop identification

In this study, an evaluation of fuzzy-based classifiers for specific crop identification using multi-spectral temporal data spanning over one growing season has been carried out. The temporal data sets have been georeferenced with 0.3 pixel rms error. Temporal information of cotton crop has been incorporated through the following five indices: simple ratio (SR), normalized difference vegetation index (NDVI), transformed normalized difference vegetation index (TNDVI), soil-adjusted vegetation index (SAVI) and triangular vegetation index (TVI), to study the effect of indices on classified output. For this purpose, a comparative study between two fuzzy-based soft classification approaches, possibilistic c-means (PCM) and noise classifier (NC), was undertaken. In this study, advanced wide field sensor (AWiFS) data for soft classification and linear imaging self scanner sensor (LISS III) data for soft testing purpose from Resourcesat-1 (IRS-P6) satellite were used. It has been observed that NC fuzzy classifier using TNDVI temporal index – dataset 2, which comprises four temporal images performs better than PCM classifier giving highest fuzzy overall accuracy of 96.03%.     

Distribution of heavy minerals in Nizampatnam-Lankavanidibba coastal sands,Andhra Pradesh,East Coast of India

Heavy mineral analysis was carried out for the beach and fore dune sediments along 60 transects of Nizampatnam-Lankavanidibba coastal area. The heavy mineral assemblage in this area with decreasing abundance of opaques (Ilmenite + magnetite, 47.67%), pyriboles (20.35%), garnets (3.66%), epidote (3.23%) and with less than 3.0% concentration of sillimanite, zircon, staurolite, kyanite, apatite, spinel, monazite, biotite, topaz, leucoxene and chlorite. The heavy mineral concentrations are high in the finer fractions i.e., +120 and +230 (ASTM) than the coarse fraction (+60) of sand. In the seven sectors, heavy mineral assemblage is same but their concentrations are different. The sectors nearer to the river mouth contain high concentration of high specific gravity heavy minerals (ilmenite and magnetite) than sectors away from the river mouth. The redistribution of heavy minerals is controlled by creek dynamics, longshore currents, size and specific gravity of the heavy minerals.     

Geochemical exploration for hydrocarbons in the soils of southeast of Krishna-Godavari Basin, Andhra Pradesh

Surface adsorbed gas surveys and geo-microbiological surveys are known techniques of petroleum exploration and aim towards risk reduction in exploration by way of identifying the areas warm with hydrocarbons and to establish intense exploration priorities amongst the identified warm areas. The present investigation aims to explore correlation between the adsorbed gas distribution pattern with the distribution of the counts of methane, ethane, propane and butane microbial oxidizers in the sub soil samples to establish the role of the latter in identifying the upward migration of hydrocarbons especially in the known petroliferous Krishna-Godavari Basin, India. A total of 135 soil samples were collected near oil and gas fields of Tatipaka, Pasarlapudi areas of Krishna Godavari Basin, Andhra Pradesh. The soil samples were collected from a depth of 2?C2.5 m. The samples collected, were analyzed for indicator hydrocarbon oxidizing bacteria, adsorbed light gaseous hydrocarbons and carbon isotopes (??¹³C_methane). The microbial prospecting studies showed the presence of high bacterial population for methane (3.94 × 10⁵ cfu/gm), ethane (3.85 × 10⁵ cfu/gm), propane (4.85 × 10⁵ cfu/gm) and butane oxidizing bacteria (3.63 × 10⁵ cfu/gm) in soil samples indicating microseepage of hydrocarbons. The light gaseous hydrocarbon analysis showed 83 ppb, 92 ppb, 134 ppb, 187 ppb and 316 ppb of C₁, C₂, C₃, nC₄ and nC₅, respectively, and the carbon isotopic composition of ??¹³C₁ of the samples ranged between ? 36.6 ?? to ?22.7?? (Pee Dee Belemnite) values, which presents convincing evidence that the adsorbed soil gases collected from these sediments are of thermogenic origin. Geo-microbial prospecting method and adsorbed soil gas and carbon isotope studies have shown good correlation with existing oil/ gas fields of K.G basin. Microbial surveys indicating microseepage of hydrocarbons can, therefore, independently precede other geochemical and geophysical surveys to delineate areas warm with hydrocarbons and mapped microbiological anomalies may provide focus for locales of hydrocarbon accumulation in the K.G basin.