海洋沉积物孔隙水中阴阳离子含量的离子色谱法分析方法

分别采用Metrosep A Supp4-250型阴离子柱和Metrosep C 2-150型阳离子柱,以1.8 mmol/L Na2CO3 1.7 mmol/L NaHCO3和4 mmol/L酒石酸 0.75 mmol/L吡啶二羧酸为阴阳离子淋洗液,用离子色谱仪来测定海洋沉积物孔隙水中阴离子Cl-、Br-和SO42-浓度和阳离子Na 、NH4 、K 、Ca2 、Mg2 的浓度。特别是针对高Cl-和高Na 背景下Br-和NH4 的测定做了专门分析,得到了较理想的结果,建立了一套离子色谱法测定海洋沉积物孔隙水中常见的阴阳离子含量的实验方法。     

大连海域近海面湍流结构及谱特征

利用黄海北部大连海域2009年10月—11月的近海面大气湍流观测资料,对湍流强度、方差相似关系、风速与温度分量谱及湍流通量协谱等进行了分析。结果表明:该海域在海风环境下湍流强度随着风速的增大逐渐减小,且强度均小于0.5;湍流方差相似关系基本上满足1/3定律,近中性环境下无量纲化三维风速方差主要受海滨地形的影响;各速度分量谱在惯性副区符合理论上的-2/3次幂规律,不稳定条件下由于湿度因素的影响,温度谱谱线在高频部分收缩较差,略有上翘;动量谱在惯性副区更接近-2/3幂率,感热谱在惯性区基本符合-4/3的分布,但是谱线分布与稳定度没有明显的规律性。     

下刚果—刚果扇盆地断裂特征及其对油气成藏的影响

下刚果—刚果扇盆地油气资源丰富、油气成藏条件优越。但由于受到盐岩的活动及区域构造应力场的作用,形成了复杂的断裂系统。此文系统分析了下刚果—刚果扇盆地的断裂特征及其对油气成藏的影响,研究认为该区平面上主要发育5个断裂带,纵向上主要发育上下两套断裂系统。断裂的形成和演化可分为三个期次,相对应可将断层分为三个级别的断层。断层的形成机制主要有盐活动及盐构造、重力滑脱作用以及古地貌格局。烃源岩的排烃时间与第三期断层活动时间相匹配,非常有利于油气沿断层进行垂向和斜侧向运移。分析认为研究区主要发育沿断阶带—碳酸盐岩运聚成藏模式、沿断层垂向运聚成藏模式以及沿盐下砂体—盐窗和Focus点运聚成藏模式共三种成藏模式。断层封堵较好,油源断层高度决定了油气运移高度和油气田规模,直接控制着油气的分布层系及规模。研究成果可指导研究区或类似地区的油气勘探。     

Dissolved inorganic carbon (DIC) and its carbon isotopic composition in sediment pore waters from the Shenhu area,northern South China Sea

The Shenhu area is one of the most favorable places for the occurrence of gas hydrates in the northern continental slope of the South China Sea. Pore water samples were collected in two piston cores (SH-A and SH-B) from this area, and the concentrations of sulfate and dissolved inorganic carbon (DIC) and its carbon isotopic composition were measured. The data revealed large DIC variations and very negative δ ¹³C-DIC values. Two reaction zones, 0–3 mbsf and below 3 mbsf, are identified in the sediment system. At site SH-A, the upper zone (0–3 mbsf) shows relatively constant sulfate and DIC concentrations and δ ¹³C-DIC values, possibly due to bioturbation and fluid advection. The lower zone (below 3 mbsf) displays good linear gradients for sulfate and DIC concentrations, and δ ¹³C-DIC values. At site SH-B, both zones show linear gradients, but the decreasing gradients for δ ¹³C-DIC and SO₄ ²⁻ in the lower zone below 3 mbsf are greater than those from the upper zone, 0–3 mbsf. The calculated sulfate-methane interface (SMI) depths of the two cores are 10.0 m and 11.1 m, respectively. The depth profiles of both DIC and δ ¹³C-DIC showed similar characteristics as those in other gas hydrate locations in the world oceans, such as the Blake Ridge. Overall, our results indicate an anaerobic methane oxidation (AMO) process in the sediments with large methane flux from depth in the studied area, which might be linked to the formation of gas hydrates in this area.     

秋季南黄海网采浮游生物的生物量谱

对2006年9月南黄海浮游生物网(孔径为70,160,505μm)采集样品内的浮游生物个体大小的粒径分布进行研究,确定各粒级大小的功能群组成,建立2006年秋季调查水域网采浮游生物的生物量谱,比较分析三个特征水域(黄海近岸、黄海中部及黄海和东海交汇区)的浮游生物生物量谱特征参数的异质性。结果表明:三种网采浮游生物粒级范围主要包括100 pg/个~70 ng/个的浮游植物和70 ng/个~62 mg/个的浮游动物。Sheldon型生物量谱为近似连续的波动曲线,标准型生物量谱为线型。总测区的标准生物量谱斜率和截距为-0.74和18.64,各个特征水域,黄海中部为-0.67和15.60、黄海近岸为-0.64和14.34、黄海、东海交汇区为-0.73和18.03。浮游动物种类多样性对标准生物量谱的特征参数具有较显著的影响。     

江苏海岸中部近岸冬季潮汐和潮流特征

南黄海西侧的江苏海岸近岸区域,素以地形复杂、潮流强劲、悬沙输运剧烈著称,但是较长期的同步潮位和潮流观测数据仍然缺乏,尤其是在近岸(20 km)浅水(20 m)区域。2014年1月在大丰港附近开展了连续潮位和潮流观测,获得的数据揭示了一系列特征。此地潮汐潮流为正规半日潮,浅水分潮显著。平均潮差为3.05 m,最显著的两个分潮为M2和S2分潮,振幅分别为1.45 m和0.52 m。潮流最显著的半日分潮M2分潮和最显著的浅水分潮M4分潮在沿岸方向上振幅分别为0.84m/s和0.12m/s,在跨岸方向上振幅分别为0.24 m/s和0.01 m/s,沿岸方向占绝对优势。潮波的沿岸传播介于前进波和驻波之间,驻波的特征稍强。M2分潮潮流椭圆最大流(长轴)方向为南偏东7.4°。存在冬季沿岸向北的余流,垂向平均值的大小为2.2 cm/s。以上潮汐潮流特征为该区域海洋物质输运研究提供了基础资料。     

扬子浅滩东南海域海底潮流沙脊、沙波特征

利用seabat8101多波束系统对扬子浅滩东南海底地形进行了高精度探测。发现海底呈明显近乎平行排列的条带状起伏,脊谷相间,沙脊大部分呈NW-SE向排列,发育在45~50 m水深范围之内,沙脊横剖面不对称,大部分沙脊西南侧坡度大、东北坡缓。沙脊规模略有差异,沙脊宽度约0.6~9.8 km,沙脊之间间距可达8.9~22.4 km,高度约1.8~13.3 m,研究区内最长可达53 km。部分脊槽过渡区域发育成片链状海底沙波,沙波大致呈NE-SW走向,波高约0.3~1 m,波长1 km左右。研究区中西部有海底礁石孤立地突兀于相对平坦的海底之上,暂定名为扬礁,最浅水深35.9 m,位于30°59'7.4'~31°N,124°36'48.7'~124°37'40'E。扬子浅滩东南海域沙脊是介于活动沙脊和衰亡沙脊之间的准活动沙脊。该研究将为我国海洋开发和海洋经济发展、海洋行政管理以及海洋安全保障提供服务。     

利用限制性最大似然方法和贝叶斯方法估计大菱鲆生长性状的遗传参数。

Bayesian and restricted maximum likelihood(REML) approaches were used to estimate the genetic parameters in a cultured turbot Scophthalmus maximus stock. The data set consisted of harvest body weight from 2 462progenies(17 months old) from 28 families that were produced through artificial insemination using 39 parent fish. An animal model was applied to partition each weight value into a fixed effect, an additive genetic effect, and a residual effect. The average body weight of each family, which was measured at 110 days post-hatching, was considered as a covariate. For Bayesian analysis, heritability and breeding values were estimated using both the posterior mean and mode from the joint posterior conditional distribution. The results revealed that for additive genetic variance, the posterior mean estimate( δ_a~2=9 320) was highest but with the smallest residual variance,REML estimates( δ_a~2=8 088) came second and the posterior mode estimate( δ_a~2=7 849) was lowest. The corresponding three heritability estimates followed the same trend as additive genetic variance and they were all high. The Pearson correlations between each pair of the three estimates of breeding values were all high,particularly that between the posterior mean and REML estimates(0.996 9). These results reveal that the differences between Bayesian and REML methods in terms of estimation of heritability and breeding values were small. This study provides another feasible method of genetic parameter estimation in selective breeding programs of turbot.     

中国沿海多鳞四指马鲅(Eleutheronema rhadinum)与四指马鲅(E. tridactylum)形态与遗传位点差异分析

多鳞四指马鲅(Eleutheronema rhadinum)与四指马鲅(Eleutheronema tridactylum)是四指马鲅属(Eleutheronema)中的名贵经济鱼类,具有良好的养殖开发前景。本文对中国沿海多鳞四指马鲅与四指马鲅群体的26个形态特征值进行了多变量比较分析,提取了7个主成分,累计贡献率达到91.112%,前三个主成分贡献率分别为28.759%、21.467%、15.469%。多鳞四指马鲅与四指马鲅形态差异主要体现在头背部、尾柄部、背腹轴及头尾轴的特征。利用4个形态学参数建立了2群体判别公式,综合判别准确率高达100%,可作为多鳞四指马鲅与四指马鲅物种资源的初步判定方法。另外,利用简化基因组测序开发出多鳞四指马鲅与四指马鲅SNP标记69207个、In Del标记12884个,丰富了四指马鲅属鱼类遗传结构研究数据。本研究为进一步开展四指马鲅数属物种资源评估与保护、选育、功能基因研究提供基础资料。     

pH值对单环刺螠呼吸排泄的影响

为研究pH值对单环刺螠(Urechis unicinctus)呼吸排泄的影响,采用静水密封法,研究了不同pH值(5、6、7、8、9)对体质量5.65±1.06g单环刺螠耗氧率、排氨率、能量代谢率、O/N值的影响.结果表明,pH值对单环刺螠耗氧率、排氨率、能量代谢率、O/N值均有显著影响(p0.05).单环刺螠耗氧率随pH值的升高而增大,pH值为8时达到最大值,之后随pH值的升高而减小.单环刺螠耗氧率与pH值之间的拟合方程为:OR=-2.977X2+55.188X-176.380,R2=0.937.单环刺螠排氨率的变化与耗氧率相似,在pH值为7时排氨率达到最大值;pH值为9时排氨率最小,与pH值为8的组差异不显著(p0.05).排氨率与pH值之间的拟合方程为:NR=-0.350X2+4.442X-6.822,R2=0.724.单环刺螠能量代谢率、O/N值均随pH值的升高呈先增大后减小的趋势,拟合方程分别为:M=-0.040X2+0.424X-0.044,R2=0.937;A=0.053X2+1.879X-7.470,R2=0.901.pH值为8的环境中,单环刺螠体内蛋白质代谢水平最低,有利于蛋白质的积累.研究表明,单环刺螠在一定pH值范围内可以通过调整生理代谢水平适应水体pH值,其适宜生活在弱碱性水中,生存最适pH值为8.