胶州湾沉积物中石油降解菌的降解能力及多样性研究

2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。     

Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea,China

Understanding the feeding selectivity on phytoplankton by shellfish is currently a big challenge.In order to investigate the feeding behavior of bay scallop(Argopecten irradians)on phytoplankton,we compared its compositions of phytopigments in digestive glands with those in the surrounding seawater,and conducted five consecutive investigations between July and November 2016 in a bay scallop culture area along coast of Qinghuangdao City,northwest of the Bohai Sea,China.Phytopigments in four-size fractionated phytoplankton of seawater(micro-(20-200 μm);nano(L)-[10-20 μm];nano(S)-[2.7-10 μm],and pico-[2.7 μm])and digestive glands of A.irradians were examined to investigate the selective feeding of A.irradians.Results show that fucoxanthin and peridinin constituted the major part of taxonomically diagnostic carotenoids(TDCs)in the micro-and nano(L)-phytoplankton in seawater.Compared with total phytoplankton biomass of seawater(TPB,sum of the four sizes),a substantial decrease of fucoxanthin proportion to total DCs in digestive glands was observed while that of peridinin,19'-butanoyloxyfucoxanthin,alloxanthin and 19'-hexanoyloxy-fucoxanthin showed an obvious increase when those pigments were mainly confined to micro-sized phytoplankton(20-200 μm).However,zeaxanthin and prasinoxanthin were mainly confined to nano(s)-and pico-phytoplankton,of which the proportions in digestive glands were usually lower in TPB.The contribution of lutein to total DCs in digestive glands(with an average of 7.23%)increased compared with TPB of seawater(with an average of 0.63%)during all five sampling times.     

Long-term monitoring of the individual self-feeding behavior of rainbow trout Oncorhynchus mykiss

Self-feeding device is extensively used in aquaculture farms, but for salmonids the individual feeding behavior has seldom been continuously observed. In this article, the individual self-feeding behavior of 10 rainbow trout was continuously monitored with a PIT tag record for 50 days with three replicates. The fish fell into three categories according to their feeding behavior, i.e. high triggering fish (trigger behavior more than 25% of the group, HT), low triggering fish (1%–25%, LT) and zero triggering fish (less than 1%). The results showed that in a group of 10 individual 1–2 HT fish accounted for most of the self-feeding behavior (78.19%–89.14%), which was far more than they could consume. The trigger frequency of the fish was significantly correlated with the initial body weight (P <0.01), however, no significant difference in growth rate among the HT, LT, and ZT fish was observed (P >0.05). Cosinor analysis showed that the two HT fish in the same group had similar acrophase. Though some of the HT fish could be active for 50 d, there were also HT fish decreased triggering behavior around 40 d and the high trigger status was then replaced by other fish, which was first discovered in salimonds. Interestingly, the growth of the group was not affected by the alternation triggering fish. These results provide evidence that in the self-feeding system the HT fish didn’t gain much advantage by their frequent self-feeding behavior, and high trigger status of the HT fish is not only an individual character but also driven by the demand of the group. In the self-feeding system, the critical individual should be closely monitored.

     

广州城市流浪乞讨者的空间管治与日常生活实践

以广州流浪乞讨者为研究对象,通过深度访谈等质性研究方法,解读针对流浪乞讨者的空间管治政策与手段,分析流浪乞讨者的日常生活实践与生境协商。研究发现：在地方城市政策对流动性与公共秩序限制的双重压力下,在流浪乞讨者的日常生活实践中,不仅有针对管治行动者而刻意为之的特殊营建,亦有基于程式化生活流程对自我身份认同的重塑与真情实感的展演。地方政府基于空间目标的救助与真实存在的日常生活实践存在着一种张力。研究关注了城市边缘群体日常生活对地理意义的主动塑造过程,对全面理解并规范管理中国城市流浪乞讨现象具有借鉴意义。     

A high-precision heuristic model to detect home and work locations from smart card data

Smart card-automated fare collection systems now routinely record large volumes of data comprising the origins and destinations of travelers. Processing and analyzing these data open new opportunities in urban modeling and travel behavior research. This study seeks to develop an accurate framework for the study of urban mobility from smart card data by developing a heuristic primary location model to identify the home and work locations. The model uses journey counts as an indicator of usage regularity, visit-frequency to identify activity locations for regular commuters, and stay-time for the classification of work and home locations and activities. London is taken as a case study, and the model results were validated against survey data from the London Travel Demand Survey and volunteer survey. Results demonstrate that the proposed model is able to detect meaningful home and work places with high precision. This study offers a new and cost-effective approach to travel behavior and demand research.     

深反射地震成像揭示的班公湖 怒江缝合带中段Moho断阶及其大地构造意义

揭示班公湖- 怒江(班怒)缝合带Moho(莫霍面)结构对于认识中特提斯洋壳俯冲和南羌塘坳陷成因具有重要地球动力学意义。基于横跨班怒缝合带的深反射地震数据(88°30′E),本文采用了中长波长静校正、噪声压制、优化叠加和叠前深度偏移(PSDM)等地震处理技术,获得了深度域地震反射偏移剖面、层速度场和高分辨率Moho结构。由深度域剖面显示,班怒缝合带Moho位于地表以下65～80 km,呈不连续北向抬升趋势,指示在拉萨地块与南羌塘地块之间存在岩石圈上地幔断阶,最大阶步可达15 km。综合分析缝合带两侧的Moho形态认为,这些断阶受南侧拉萨地体的岩石圈上地幔以19. 5°北倾俯冲与北侧南羌塘地块的上地壳抬升驱动,可能与深部存在局部熔融相关。班怒缝合带下的Moho结构表明,随着晚侏罗世—早白垩世中特提斯洋闭合,南羌塘地体由边缘海沉积向前陆盆地转换,形成南羌塘坳陷。     

基墨里造山与松潘-甘孜锂矿链成因的探讨

晚三叠世—早侏罗世,基墨里大陆和泛华夏陆块与欧亚大陆的碰撞,形成了4000 km 长的基墨里造山带。位于三大陆之间、现今青藏高原北部的松潘 甘孜地体的造山属性的确定,对于探索松潘 甘孜锂矿链的成因有重要意义。松潘 甘孜地体西部和北部的早侏罗世陆相火山岩、底砾岩和煤系地层(~201 Ma)不整合在晚三叠世褶皱地层及花岗岩体之上,为晚三叠世—早侏罗世的基墨里造山时限提供了直接证据。松潘 甘孜地体中的甲基卡、可尔因、扎乌龙和白龙山 大红柳滩等稀有金属矿集区具有共同特征：赋存在由核部高分异S型花岗岩和幔部中晚三叠世浊积岩组成的片麻岩穹隆构造中、经历了巴罗 巴肯变质作用、含锂伟晶岩脉侵位在花岗岩体上部的伸展空间。通过对松潘 甘孜地体区域地质调查和对矿集区的变质、变形、岩浆和成矿作用及同位素年代学研究,提出该地体经历晚三叠世—早中侏罗世的基墨里造山过程：① 230~220 Ma：地壳缩短和加厚阶段,以盖层大规模强烈褶皱、逆冲带,盖层与基底之间向南的滑脱变形为特征,伴随深熔和巴罗式变质作用;② 220~190 Ma：地壳减压折返阶段,大量花岗岩侵位在中晚三叠世浊积岩中、形成片麻岩穹隆并伴随巴肯式变质作用。由于花岗质岩浆的高度分异及岩浆不混溶作用,导致侵位在片麻岩穹隆顶部伸展空间的伟晶岩稀有金属富集成矿。此外,伟晶岩型锂矿科学钻探(JSD)揭示了甲基卡矿集区多层次穹状花岗岩席控制含锂伟晶岩脉的成矿机制,推测大规模中下地壳基底深熔驱动岩浆上升,岩浆体沿上地壳中的构造面推叠形成岩席。     

近50年石家庄地-气温差变化特征

利用石家庄17个国家气象站1972—2021年逐日地面气温、0 cm地温资料,分析了石家庄地-气温差的变化特征,结果表明：(1)石家庄地-气温差从1月开始逐渐增加,5月达到最大值5.0℃,然后开始减小,12月达到最小值-0.8℃;地-气温差在11月到次年1月为负值;春、夏、秋季均为正值,夏季最大,春季大于秋季,冬季以负值为主;(2)石家庄多年平均地-气温差在1.6～2.6℃之间,平均为2.1℃;整体上东部大于西部。(3)近50年石家庄年平均地-气温差呈显著的减小趋势,变化速率为-0.14℃/10a;夏、秋、冬三季的减小趋势均非常显著,夏季的减小趋势最强;石家庄市区和近郊站点年和四季地-气温差的减少趋势更显著。本文结论对科学认识石家庄城市生态环境的变化具有参考意义。     

Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...     

青岛琅琊湾滨海湿地的保护和利用

随着沿海经济的飞速发展,沿海土地后备资源出现日益紧缺的局面,滨海湿地资源的保护刻不容缓。目前正在琅琊湾西海岸进行的青岛港董家口港区等重工业建设,对滨海湿地破坏严重,亟需对琅琊湾滨海湿地做出科学规划和合理利用,建议设立湿地-工业缓冲带,建设人工湿地和开展立体养殖。