利用光变曲线估计猎鹰九号火箭末级的旋转状态研究

光度观测是地基观测空间目标的主要手段之一,利用光度信息能够估计空间目标的相关特征信息.为了更好地了解空间目标的旋转状态,选取具有代表性的猎鹰九号火箭末级作为研究对象,由其光变信息研究旋转状态.首先利用云南天文台1.2 m光学望远镜获取猎鹰九号火箭末级的光度数据,再对目标星等进行斜距归一化,得到目标光变信息并分析目标星等...     

HPABM: A Hierarchical Parallel Simulation Framework for Spatially-explicit Agent-based Models

A Hierarchical Parallel simulation framework for spatially-explicit Agent-Based Models (HPABM) is developed to enable computationally intensive agent-based models for the investigation of large-scale geospatial problems. HPABM allows for the utilization of high-performance and parallel computing resources to address computational challenges in agent-based models. Within HPABM, an agent-based model is decomposed into a set of sub-models that function as computational units for parallel computing. Each sub-model is comprised of a sub-set of agents and their spatially-explicit environments. Sub-models are aggregated into a group of super-models that represent computing tasks. HPABM based on the design of super- and sub-models leads to the loose coupling of agent-based models and underlying parallel computing architectures. The utility of HPABM in enabling the development of parallel agent-based models was examined in a case study. Results of computational experiments indicate that HPABM is scalable for developing large-scale agent-based models and, thus, demonstrates efficient support for enhancing the capability of agent-based modeling for large-scale geospatial simulation.     

对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

Differential gene expression in the body wall of the sea cucumber(Apostichopus japonicus) under strong lighting and dark conditions

Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light("light"), normal light("control") and fully dark("dark") environment. In the comparisons of "control" vs. "dark", "control" vs. "light" and "dark" vs."light", 1 161, 113 and 1 705 differentially expressed genes(DEGs) were identified following the criteria of|log_2 ratio|≥1 and FDR≤0.001, respectively. Gene ontology analysis showed that "cellular process" and "binding"enriched the most DEGs in the category of "biological process" and "molecular function", while "cell" and "cell part" enriched the most DEGs in the category of "cellular component". And the DEGs were mapped to 214, 41 and229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light.     

Co-dwelling,Mix-dwelling and Dis-dwelling: The Diversity Among Three Human and Livestock Dwelling Forms in Rural China

Humans and livestock have always dwelling together, especially in rural areas. Based on a multi-method approach of ethnographic fieldwork, grounded theory and one-way multivariate analysis of variance, this paper explores human and livestock dwelling. The findings show that human and livestock dwelling form a special type of human dwelling place, with forms defined as co-, mix- and dis-dwelling. Then, from co-, mix- to dis-dwelling constructed a story line to the evolution of human and livestock dwelling forms, which further explains the entire dwelling situation of humans and livestock, including the dimensions of human dweller, livestock dweller, the rural environment and special events. Among co-, mix- and dis-dwelling significant differences existed between human and livestock individuals, the nature of rural environment, vernacularity and rural tourism development. Between co- and dis-dwelling, significant differences existed in seventeen aspects; between mix- and dis-dwelling, significant differences existed in eleven aspects; meanwhile, no significant differences existed between co- and mix-dwelling. Accordingly, this suggested that human and livestock dwelling forms can be divided into mix- and dis-dwelling. This study on humans and livestock dwelling relationship offer a new perspective on rural and animal geography. This paper is an exploratory foray into rural dwelling geography as part of the Anthrozoology study and broadens the scope of extant scholarship on human dwelling morphology, as well as analyses the spatial relationships between human and livestock dwelling.     

基于阈值光电子-光离子符合技术的分子离子光谱和解离动力学研究

对电子和离子同时采用速度聚焦电场收集的阈值光电子-光离子符合成像谱仪能够有效提高电子的收集效率和能量分辨率.利用该符合成像谱仪,开展了Xe/Ar/Ne 惰性混合气体及NO 分子的阈值光电子谱、阈值光电子-光离子符合质谱和质量选择的符合光谱等实验研究,精确测量了NO 分子的电离势,并且获得了NO⁺离子振动态分辨的X¹Σ⁺,c³Π和B¹Π态光谱.还进一步研究了NO^{+ 相似文献}   

经济预测模型在土地规划中的应用与分析

主要介绍土地规划时需用到的几种预测模型 ,具体阐述了几种模型的原理 ,并以抚州地区的总人口历史数据为例 ,进行实际的预测与分析 ,从而总结各种模型的优缺点 ,选择最合适的土地规划预测模型。     

自适应非线性有限元的多重网格法求解及应用

描述了非线性自适应有限元的多重网格法求解过程。 通过算例说明了方法的有效性以及自适应多重网格有限元用于开挖施工过程模拟分析的优越性。