Cytotoxic and genotoxic evaluation and chemical characterization of sewage treated using activated sludge and a floating emergent-macrophyte filter in a municipal wastewater treatment plant: a case study in Southern Brazil

This study was conducted to evaluate the chemical parameters and the cytotoxic and genotoxic potential of raw domestic sewage and effluents from treatment with activated sludge and a floating emergent-macrophyte filter from a domestic wastewater treatment plant in the city of Novo Hamburgo, Rio Grande do Sul, Brazil. The physicochemical analysis revealed that both treatment systems achieved the legal emission pattern for biochemical oxygen demand, chemical oxygen demand, and suspended solids, but ammoniacal nitrogen and E. coli values were above the limits in the macrophyte treatment effluent. Phosphorous values were above the maximum permitted for both treatments. The results obtained from the Allium cepa test and the micronuclei test in fish did not demonstrate any significant differences in both cytotoxicity (mitotic index) and genotoxicity (chromosome aberration and micronucleus) endpoints between the negative control group and the exposed groups. However, the comet assay in fish revealed a DNA damage increase in animals exposed to the 30 % concentration of the macrophyte effluent and two concentrations of the activated sludge treatment effluent (10 and 75 %), which suggests that these two treatment systems may increase wastewater genotoxicity.     

Pilocarpus microphyllus seedling growth threatened by climate change: an ecophysiological approach

Theoretical and Applied Climatology - The climate change endangers many species of the Amazon Forest. An example, in the endangered medicinal species Pilocarpus microphyllus (popularly known as...     

Parameter Optimization of Activated Carbon Production from Agave sisalana and Punica granatum Peel: Adsorbents for C.I. Reactive Orange 4 Removal from Aqueous Solution

In the present study, activated carbons were prepared from sisal fiber (Agave sisalana sp.) and pomegranate peel (Punica granatum sp.) using phosphoric acid as the activating agent. Both sisal fiber activated carbon (SFAC) and pomegranate peel activated carbon (PPAC) were characterized using methylene blue number, iodine number, BET surface area, SEM, and FTIR. The BET surface area of the SFAC and PPAC were 885 and 686 m²/g, respectively. The adsorption studies using C.I. Reactive Orange 4 dye on the SFAC and PPAC were carried out. The effects of time, initial adsorbate concentration, pH, and temperature on the adsorption were studied. The isotherm studies were carried and it was found that the Langmuir and Freundlich isotherms fit well for the adsorption of RO 4 on SFAC, while adsorption of RO 4 on PPAC is better represented by the Langmuir and Temkin isotherms. Adsorption kinetics of adsorption was determined using pseudo first order, pseudo second order, Elovich and intraparticle diffusion models and it was found that the adsorption process follows pseudo second order model. Thermodynamics parameters such as changes in free energy (ΔG), enthalpy (ΔH), and entropy (ΔS) were determined by using van't Hoff equation. The positive ΔH value indicates that RO 4 dye adsorption on SFAC and PPAC is endothermic in nature.     

Estimation of the Rock Deformation Modulus and RMR Based on Data Mining Techniques

In this work Data Mining tools are used to develop new and innovative models for the estimation of the rock deformation modulus and the Rock Mass Rating (RMR). A database published by Chun et al. (Int J Rock Mech Min Sci 46:649–658, 2008) was used to develop these models. The parameters of the database were the depth, the weightings of the RMR system related to the uniaxial compressive strength, the rock quality designation, the joint spacing, the joint condition, the groundwater condition and the discontinuity orientation adjustment, the RMR and the deformation modulus. As a modelling tool the R program environment was used to apply these advanced techniques. Several algorithms were tested and analysed using different sets of input parameters. It was possible to develop new models to predict the rock deformation modulus and the RMR with improved accuracy and, additionally, allowed to have an insight of the importance of the different input parameters.     

Geochemical study of submicron particulate matter(PM1)in a metropolitan area

Air pollution has become a major problem in urban areas due to increasing industrialization and urbanization.In this study ambient concentrations of PM1 and metal concentrations as well as source contributions were identified and quantified by using Positive Matrix Factorization(PMF)in receptor modeling in the Metropolitan Area of Porto Alegre,Brazil.The PM1 samples were collected on PTFE filters from December 2012 to December 2014 in two sampling sites.Major ion and trace element concentrations were assessed.The average concentrations were 12.8 and 15.2μg/m³ for Canoas and Sapucaia do Sul sites,respectively.Major ion contributions of PM1 were secondary pollutants such as sulfate and nitrate.Trace elements,especially Cu,Pb,Zn,Cd,and Ni also made important contributions which are directly associated with anthropogenic contributions.Our results show significantly higher levels in winter than in summer.Most of the PM1 and the analyzed PM species and elements originated from anthropogenic sources,especially road traffic,combustion processes and industrial activities,which are grouped in 7 major contributing sources.A back-trajectory analysis showed that the long-range transport of pollutants was not relevant in relation to the contribution to PM1 and metal concentrations.This work highlights the importance of urban planning to reduce human health exposure to traffic and industrial emissions,combined with awareness-raising actions for citizens concerning the impact of indoor sources.     

On Monin–Obukhov Scaling in and Above the Atmospheric Surface Layer: The Complexities of Elevated Scintillometer Measurements

In scintillometry Monin–Obukhov similarity theory (MOST) is used to calculate the surface sensible heat flux from the structure parameter of temperature (C_T²){(C_{T^2})} . In order to prevent saturation a scintillometer can be installed at an elevated level. However, in that case the observation level might be located outside the atmospheric surface layer (ASL) and thus the validity of MOST questioned. Therefore, we examine two concepts to determine the turbulent surface sensible heat flux from the structure parameter at elevated levels with data obtained at 60-m height on the Cabauw tower (the Netherlands). In the first concept (MOSTs) C_T²{C_{T^2}} is still scaled with the surface flux, whereas in the second (MOSTl) C_T²{C_{T^2}} is scaled with the local sensible heat flux. The C_T²{C_{T^2}} obtained from both concepts is compared with direct observations of C_T²{C_{T^2}} using a sonic anemometer/thermometer. In the afternoon (when the measurement height is located within the ASL) both concepts give results that are comparable to the directly observed values of C_T²{C_{T^2}} . In the morning (data outside the ASL), our data do not unequivocally support either of the two concepts. First, the peak in C_T²{C_{T^2}} that occurs when the measurement height is located in the entrainment zone disqualifies the use of MOST. Second, during the morning transition, local scaling shows the correct pattern (zero flux and a minimum in C_T²{C_{T^2}}) but underestimates C_T²{C_{T^2}} by a factor of ten. Third, from the best linear fit a we found that the slope of MOSTl gave better results, whereas the offset is closer to zero for MOSTs. Further, the correlation between the direct observations and MOST-scaled results is low and similar for the two concepts. In the end, we conclude that MOST is not applicable for the morning hours when the observation level is above the ASL.     

A simplified approach for flood vulnerability assessment of historic sites

In the last decades, floods have increased in frequency all over the world due to diverse phenomena such as climate change, extended urbanization, land use, etc. Their social, cultural, economic and environmental impacts have also grown significantly, highlighting the need for the development of further studies and improved methods to manage and mitigate flood risk, mainly in urban areas. Historic sites need particular attention in this field, not only because the high and irreplaceable cultural value of these areas, but also taking into account that the constructive typologies that they host are particularly vulnerable to natural hazards. In consequence of that, the analysis of the phenomena, the evaluation of their consequences and the adoption of adequate mitigation and preparedness measures are presently a fundamental societal challenge. Having this in mind, the present paper aims at proposing an innovative methodology focused on the assessment of flood vulnerability in historic sites through the evaluation of a set of exposure and sensitivity indicators. From the analysis of these indicators, it is possible to obtain a Flood Vulnerability Index capable of measuring the spread of flood vulnerability over an extended area. The historic centre of Guimarães, in Portugal, declared by UNESCO as a World Heritage Site in 2001, is used here as a pilot case study to apply and discusses the preliminary version of the approach. Although some improvements are still needed, this approach can be already used to provides preliminary vulnerability scenarios and to point the way to the definition of more efficient and customized strategies for managing and mitigating flood risk in historic sites. Moreover, with further improvements and calibrations resorting to larger and more diverse data, it will be possible to reduce some of the uncertainties currently involved in the assessment process and to make its application wider and more robust.

     

A method predicting the earthquake-induced landslide risk by back analyses of past landslides and its application in the region of the Wenchuan 12/5/2008 earthquake

The work considers critical slopes located at regions with high danger and proposes a method which approximately predicts the risk of earthquake-induced excessive movement of these slopes, in the case where relevant soil strength data along their slip surface do not exist. The method utilizes (1) simplified constitutive equations predicting soil response along slip surfaces and (2) a multi-block sliding system model, both recently proposed. It involves the following steps: (a) collect relevant topographic information and laboratory test results of past landslides at the region of interest, (b) (i) analyze the relevant laboratory tests to estimate the soil constitutive parameters and (ii) estimate the mobilized residual soil strength by back analysis of relevant past landslides, (c) select constitutive model parameters for future applications at the region based on step (b) and validate them based on analysis of the triggering of the past landslides and (d) apply the selected constitutive model parameters in critical slopes in the region under consideration to predict not only whether triggering occurs, but also the post-triggering slide deformation. The paper, after describing in detail the proposed method, applies it at the Wenchuan region, where the 12th May 2008 earthquake triggered many landslides. During the application, four ring shear test results and twelve landslides of the 12th May 2008 earthquake were considered. The application was successful and the following were observed: (i) in the back analyses, the multi-block model predicted reasonably well the final configuration of all slides, (ii) apart from two significantly larger back-estimated values of the residual soil strength, small scatter existed in the other back-estimated values and (iii) the selected model parameters were duly validated under step (c). Furthermore, as the back-estimated friction angle of most landslides was less than 18°, and the materials along the slip surface have a Liquid Limit value less than 25 %, it is inferred that some, or all of the slip surface during these slides, was sheared in an undrained manner.