三种不同乳酸菌对凡纳滨对虾生长、抗病力及肠道菌群结构的影响

本研究比较了饲料中添加粪肠球菌(Enterococcus faecalis LYB1)、嗜酸乳杆菌(Lactobacillus acidophilus W2)和凝结芽孢杆菌(Bacillus coagulans NJ17)对凡纳滨对虾(Litopenaeus vannamei)幼虾的生长、抗病力及肠道菌群结构的影响。以基础饲料中分别添加1.0×1010 cfu/kg浓度的3种不同乳酸菌为实验组,以基础饲料为对照组,以投喂方式养殖对虾42 d。养殖结束后,进行14 d的副溶血弧菌(Vibrio parahaemolyticus)攻毒实验。研究表明:投喂3种乳酸菌的实验组对虾的体重、特定生长率、饲料效率和表观消化率均显著高于对照组(P<0.05),其中以添加凝结芽孢杆菌组效果最佳。攻毒实验显示,添加粪肠球菌和嗜酸乳杆菌均可显著降低对虾的累计死亡率(P<0.05)。添加乳酸菌的各实验组对虾的肠道细菌群落结构与对照组相比均发生了明显变化,其中以添加粪肠球菌组差异最大。在门水平上,变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)为对虾肠道优势菌群。在纲分类水平上,α-变形菌纲(Alphaproteobacteria)、丹毒丝菌纲(Erysipelotrichia)和黄杆菌纲(Flavobacteriia)为对虾肠道优势菌群。研究结果显示,凝结芽孢杆菌NJ17对凡纳滨对虾促长效果最佳,而嗜酸乳杆菌W2对对虾抗病力提高效果最佳,3种乳酸菌均明显改善了对虾肠道微生物群落的组成和丰度。     

基于网络药理学和分子对接探讨左金丸治疗肝癌的作用机制

目的：运用网络药理学和分子对接方法探讨左金丸治疗肝癌的作用机制。方法：从TCMSP和BATMAN-TCM数据库获取左金丸的化合物及其相应靶点,检索GeneCards、OMIM和TTD 3个数据库获得肝癌的相关靶基因,取两者靶基因交集得到左金丸治疗肝癌的预测靶基因;运用Cytoscape 3.7.1软件构建左金丸-化合物-靶点-肝癌和PPI网络图;运用R软件对预测靶基因进行GO和KEGG富集分析;最后运用分子对接技术对关键化合物和靶点进行验证。结果：共获取左金丸35个化合物及173个相应靶点,左金丸与肝癌的共同靶点有103个。PPI结果表明AKT1、MAPK1、TP53、JUN和RELA可能为左金丸治疗肝癌的关键靶点。富集分析示左金丸可能通过乙型肝炎、卡波西肉瘤相关疱疹病毒感染、人巨细胞病毒感染、丙型肝炎、MAPK信号通路、肝癌等信号通路抗肝癌。分子对接结果示槲皮素和黄连素均能与AKT1和MAPK1稳定结合。结论：本研究初步揭示了左金丸通过多成分、多靶点、多通路治疗肝癌的作用机制,为后续左金丸治疗肝癌提供理论参考。     

印度洋海温异常对西北太平洋台风的影响及机制研究

基于近40 a NCEP/NCAR再分析月平均高度场、风场、涡度场、垂直速度场以及NOAA重构的海面温度(sea surface temperature,SST)资料和美国联合台风预警中心(Joint Typhoon Warning Center,JTWC)热带气旋最佳路径资料,利用合成分析方法,研究了前期春季及同期夏季印度洋海面温度同夏季西北太平洋台风活动的关系。结果表明:1)前期春季印度洋海温异常(sea surface temperature anomaly,SSTA)尤其是关键区位于赤道偏北印度洋和西南印度洋地区对西北太平洋台风活动具有显著的影响,春季印度洋海温异常偏暖年,后期夏季,110°～180°E的经向垂直环流表现为异常下沉气流,对应风场的低层低频风辐散、高层辐合的形势,这种环流形势使得低层水汽无法向上输送,对流层中层水汽异常偏少,纬向风垂直切变偏大,从而夏季西北太平洋台风频数偏少、强度偏弱,而异常偏冷年份则正好相反。2)春季印度洋异常暖年,西北太平洋副热带高压加强、西伸;而春季印度洋异常冷年,后期夏季西北太平洋副热带高压减弱、东退,这可能是引起夏季西北太平洋台风变化的另一原因。     

Clogging of saturated porous media by silt‐sized suspended solids under varying physical conditions during managed aquifer recharge

Managed aquifer recharge is an effective method for utilizing excess flood flows, but clogging of porous media is a limiting factor in the implementation of this water storage technique. In recent years, much research on the physical clogging of porous media during artificial recharge has been conducted. However, the understanding of clogging due to silt‐sized suspended solids (SS) is still inadequate, especially under varying physical conditions. Here, we subjected sand columns to controlled rates of flow and SS suspensions to investigate the influence of media size, SS size, SS concentration, and flow velocity on the clogging of porous media by silt‐sized SS. The results show that the diameter ratio of SS particles to sand grains is the dominant factor influencing the position of physical clogging. As pore velocity increased, the mobility of silt‐sized SS was enhanced and retention in the porous media decreased noticeably. The spatial retention profiles in the porous media were found to vary greatly at different flow velocities. The SS concentration of the infiltrating suspension also dramatically influenced the mobility and deposition of silt‐sized SS particles, such that high concentrations accelerated the clogging process. As the different physical factors changed, the breakthrough curves and retention profiles of silt‐sized SS particles changed obviously and the mechanisms of retention differed. On the whole, clogging position is mainly determined by particle size ratio, but clogging rate is dominated by a variety of factors including particle size ratio, SS concentration, and flow velocity.     

Regional extreme‐dry‐spell frequency analysis using the L‐moments method in the middle reaches of the Yellow River Basin,China

In this research, the regional extreme‐dry‐spell frequency in the middle reaches of the Yellow River Basin (YRB) is studied by the L‐moments method. The research area has been divided into three subregions (regions 1, 2 and 3), which have been identified as homogenous regions. The results of a goodness‐of‐fit test indicate that a generalized normal distribution is the optimal regional model for regions 1 and 2 whereas a generalized Pareto distribution is the optimal regional model for region 3. The return period analysis figures out that the maximum length‐of‐dry‐spell (MxDS) values increase from south to north in the southern part and increase from northeast to southwest in the northern part of the middle reaches of the YRB under different return periods. The increments of quantiles of dry spell under different return levels indicate that drought risk in region 1 is higher than that in regions 2 and 3. The analysis of the occurrence day of MxDS shows that MxDS mostly occurred during winter of 1998 and spring of 1999 in most stations during the considered period. By comparing summer MxDS events, it can be found that mean MxDS values have slightly increased in regions 1 and 2 during the last five decades. The maximum mean MxDS values appeared in the 2000s for regions 1 and 2 and in the 1990s for region 3. The atmospheric circulation shows that the positive anomaly centre in the west of North China, negative anomaly centre in the east of North China and the strong western Pacific subtropical high led to the decrease of precipitation in North China during the summer of 1997. Copyright © 2013 John Wiley & Sons, Ltd.     

The vertical influence of temperature and precipitation on snow cover variability in the Central Tianshan Mountains,Northwest China

Seasonal snow cover in mountainous regions will affect local climate and hydrology. In this study, we assessed the role of altitude in determining the relative importance of temperature and precipitation in snow cover variability in the Central Tianshan Mountains. The results show that: (a) in the study area, temperature has a greater influence on snow cover than precipitation during most of the time period studied and in most altitudes. (b) In the high elevation area, there is a threshold altitude of 3,900 ± 400 m, below which temperature is negatively correlated whereas precipitation is positively correlated to snow cover, and above which the situation is the opposite. Besides, this threshold altitude decreases from snow accumulated period to snow stable period and then increases from snowmelt period to snow‐free period. (c) Below 2,000 m, there is another threshold altitude of 1,400 ± 100 m during the snow stable period, below (above) which precipitation (temperature) is the main driver of snow cover.     

"居住不稳定性"对中国大城市流动人口健康的影响研究

随着城镇化加速和人口“流动性”增强,中国大城市流动人口的健康问题备受关注。流动人口的居住具有典型不稳定特征,分析其对流动人口身心健康的影响具有重要意义。为此,本文采用北京、深圳、上海等9个大城市的实地调研数据,运用层级回归和分组回归,探讨影响流动人口身心健康的因素,关注“居住不稳定性”因素的影响。研究表明：城市内部多次迁居和城市间频繁流动对流动人口健康具有显著负面影响;住房因素中,自有住房和选择居住在本地人居多的邻里对其健康具有显著正面影响。另外,“性别”因素具有调节效应;居住不稳定性对主观幸福感和自评生理健康的影响程度因“性别”不同而有所差异,男性健康对时空因素更为敏感和脆弱,女性健康不佳多归因于住房和邻里。     

Quantification of human and climate contributions to multi-dimensional hydrological alterations: A case study in the Upper Minjiang River,China

Dual factors of climate and human on the hydrological process are reflected not only in changes in the spatiotemporal distribution of water resource amounts but also in the various characteristics of river flow regimes. Isolating and quantifying their contributions to these hydrological alterations helps us to comprehensively understand the response mechanism and patterns of hydrological process to the two kinds of factors. Here we develop a general framework using hydrological model and 33 indicators to describe hydrological process and quantify the impact from climate and human. And we select the Upper Minjiang River(UMR) as a case to explore its feasibility. The results indicate that our approach successfully recognizes the characteristics of river flow regimes in different scenarios and quantitatively separates the climate and human contributions to multi-dimensional hydrological alterations. Among these indicators, 26 of 33 indicators decrease over the past half-century(1961–2012) in the UMR, with change rates ranging from 1.3% to 33.2%, and the human impacts are the dominant factor affecting hydrological processes, with an average relative contribution rate of 58.6%. Climate change causes an increase in most indicators, with an average relative contribution rate of 41.4%. Specifically, changes in precipitation and reservoir operation may play a considerable role in inducing these alterations. The findings in this study help us better understand the response mechanism of hydrological process under changing environment and is conducive to climate change adaptation, water resource planning and ecological construction.     

喀斯特地区地表温度空间降尺度方法初探

喀斯特地区地形起伏大,常规的降尺度方法及所选择的因子对其不适用。该文根据喀斯特地区的特点,选取反射率、遥感指数及高程因子为尺度因子,通过随机森林模型建立MODIS第31、32波段辐射亮度数据和尺度因子之间的非线性关系,构建适合喀斯特地区的随机森林(Karst Random Forest,KRF)模型,成功将空间分辨率为1 km的热红外辐射亮度降至100 m,最后利用劈窗算法反演得到空间分辨率为100 m的地表温度。将KRF方法与仅考虑常规因子的多因子随机森林回归模型(MTVRF)和热锐化算法(TsHARP)对比,结果表明:1)在不同高差的喀斯特地区,KRF方法可较大程度提高地表温度降尺度精度,均方根误差(RMSE)在遵义市西北部和贵阳市以南地区分别为2.46 K和1.45 K,较MTVRF模型分别降低了0.1419 K和0.2928 K,较TsHARP算法分别降低了0.6204 K和0.6953 K,且在地形起伏度较低的喀斯特山区城市(贵阳市以南)表现更好;2)在喀斯特地区不同地类上,KRF方法效果也较好,其中植被区域最优,RMSE为1.41 K,破碎的裸土区域RMSE为1.84 K。研究显示,考虑特殊尺度因子的KRF方法可提高喀斯特地区地表温度的降尺度精度,为该地区以地表温度为基础的研究提供更精细可靠的地表温度产品。     

基于多源数据的新疆人居环境质量评价

针对区域人居环境质量缺乏从自然和人文因子组合综合评价分析的现状,利用遥感影像、站点监测、交通网络等多源数据,获取与人居环境质量密切相关的自然和人文因素共17个影响因子。应用熵权法、GIS空间分析及现代大数据等方法,定量研究新疆人居环境质量综合评价指数的空间分异及其与经济、人口分布的协调性关系。结果表明：① 新疆自然环境本底受水资源及植被覆盖情况影响较大,自然环境本底评价指数高值区域的空间分布特征与绿洲分散分布的特点具有极大的相似性,县域尺度的高质量区聚集分布在天山北坡的伊犁河谷等地,低质量区和一般质量区大面积集中连片地分布在新疆南部和东部。② 人文环境受交通通达程度及基础支撑完善度影响较大,地级市、县级市优于其他县级地区,人文环境质量基本呈现以各地级市、县级市为中心向外辐射递减的空间分异规律。③ 人居环境质量综合评价指数空间分异显著,呈现出以乌鲁木齐、昌吉市等点为中心的局部圈层式递减的空间分异规律。木垒?皮山分界线东南部与西北部人居环境质量的平均水平相差悬殊。④ 整体人居环境质量水平较低,人口分布与人居环境质量综合评价指数空间分布的匹配度不足,一般质量区和低质量区的县域土地面积占比高达84.90%,分布了47.74%的人口,低质量区仍有较大的提升潜力。⑤ GDP水平与人居环境质量保持较高的匹配性,环境经济协调型县市数量较少,环境经济滞后型县市数量最多,经济发展与人居环境相互掣肘,制约着新疆的可持续性发展。