Effect of electron donors on the fractionation of sulfur isotopes by a marine Desulfovibrio sp.

Sulfur isotope effects produced by microbial dissimilatory sulfate reduction are used to reconstruct the coupled cycling of carbon and sulfur through geologic time, to constrain the evolution of sulfur-based metabolisms, and to track the oxygenation of Earth’s surface. In this study, we investigate how the coupling of carbon and sulfur metabolisms in batch and continuous cultures of a recently isolated marine sulfate reducing bacterium DMSS-1, a Desulfovibrio sp., influences the fractionation of sulfur isotopes.DMSS-1 grown in batch culture on seven different electron donors (ethanol, glycerol, fructose, glucose, lactate, malate and pyruvate) fractionates ³⁴S/³²S ratio from 6‰ to 44‰, demonstrating that the fractionations by an actively growing culture of a single incomplete oxidizing sulfate reducing microbe can span almost the entire range of previously reported values in defined cultures. The magnitude of isotope effect correlates well with cell specific sulfate reduction rates (from 0.7 to 26.1 fmol/cell/day). DMSS-1 grown on lactate in continuous culture produces a larger isotope effect (21-37‰) than the lactate-grown batch culture (6‰), indicating that the isotope effect also depends on the supply rate of the electron donor and microbial growth rate. The largest isotope effect in continuous culture is accompanied by measurable changes in cell length and cellular yield that suggest starvation. The use of multiple sulfur isotopes in the model of metabolic fluxes of sulfur shows that the loss of sulfate from the cell and the intracellular reoxidation of reduced sulfur species contribute to the increase in isotope effects in a correlated manner. Isotope fractionations produced during sulfate reduction in the pure culture of DMSS-1 expand the previously reported range of triple sulfur isotope effects (³²S, ³³S, and ³⁴S) by marine sulfate reducing bacteria, implying that microbial sulfur disproportionation may have a smaller ³³S isotopic fingerprint than previously thought.     

Vegetation-groundwater dynamics at a former uranium mill site following invasion of a biocontrol agent: A time series analysis of Landsat normalized difference vegetation index data

Because groundwater recharge in dry regions is generally low, arid and semiarid environments have been considered well-suited for long-term isolation of hazardous materials (e.g., radioactive waste). In these dry regions, water lost (transpired) by plants and evaporated from the soil surface, collectively termed evapotranspiration (ET), is usually the primary discharge component in the water balance. Therefore, vegetation can potentially affect groundwater flow and contaminant transport at waste disposal sites. We studied vegetation health and ET dynamics at a Uranium Mill Tailings Radiation Control Act (UMTRCA) disposal site in Shiprock, New Mexico, where a floodplain alluvial aquifer was contaminated by mill effluent. Vegetation on the floodplain was predominantly deep-rooted, non-native tamarisk shrubs (Tamarix sp.). After the introduction of the tamarisk beetle (Diorhabda sp.) as a biocontrol agent, the health of the invasive tamarisk on the Shiprock floodplain declined. We used Landsat normalized difference vegetation index (NDVI) data to measure greenness and a remote sensing algorithm to estimate landscape-scale ET along the floodplain of the UMTRCA site in Shiprock prior to (2000–2009) and after (2010–2018) beetle establishment. Using groundwater level data collected from 2011 to 2014, we also assessed the role of ET in explaining seasonal variations in depth to water of the floodplain. Growing season scaled NDVI decreased 30% (p < .001), while ET decreased 26% from the pre- to post-beetle period and seasonal ET estimates were significantly correlated with groundwater levels from 2011 to 2014 (r² = .71; p = .009). Tamarisk greenness (a proxy for health) was significantly affected by Diorhabda but has partially recovered since 2012. Despite this, increased ET demand in the summer/fall period might reduce contaminant transport to the San Juan River during this period.     

A multi-wavelength study of parent volatile abundances in Comet C/2006 M4 (SWAN)

Volatile organic emissions were detected post-perihelion in the long-period Comet C/2006 M4 (SWAN) in October and November 2006. Our study combines target-of-opportunity infrared observations using the Cryogenic Echelle Spectrometer (CSHELL) at the NASA-IRTF 3-m telescope, and millimeter wavelength observations using the Arizona Radio Observatory (ARO) 12-m telescope. Five parent volatiles were measured with CSHELL (H₂O, CO, CH₃OH, CH₄, and C₂H₆), and two additional species (HCN and CS) were measured with the ARO 12-m. These revealed highly depleted CO and somewhat enriched CH₃OH compared with abundances observed in the dominant group of long-period (Oort cloud) comets in our sample and similar to those observed recently in Comet 8P/Tuttle. This may indicate highly efficient H-atom addition to CO at very low temperature (∼10-20 K) on the surfaces of interstellar (pre-cometary) grains. Comet C/2006 M4 had nearly “normal” C₂H₆ and CH₄, suggesting a processing history similar to that experienced by the dominant group. When compared with estimated water production at the time of the millimeter observations, HCN was slightly depleted compared with the normal abundance in comets based on IR observations but was consistent with the majority of values from the millimeter. The ratio CS/HCN in C/2006 M4 was within the range measured in ten comets at millimeter wavelengths. The higher apparent H-atom conversion efficiency compared with most comets may indicate that the icy grains incorporated into C/2006 M4 were exposed to higher H-atom densities, or alternatively to similar densities but for a longer period of time.     

Magnetic susceptibility as a proxy for coal ash pollution within riverbed sediments in a watershed with complex geology (southeastern USA)

A study of near surface sediments from the Dan River (southeastern USA) was conducted to assess the use of magnetic properties as proxies of coal ash after a recent spill. The watershed geology is diverse and potentially contributes magnetic minerals to riverbed sediment from diabase dikes in the Dan River Triassic Basin and from granitic gneiss outside the basin. Coal ash is heterogeneous, including aluminosilicate spheres, amorphous particles and carbonaceous rods and lacy particles. The magnetic fraction of ash from the failed storage pond is up to 17 wt% and is mostly composed of black spheres with maghemite and magnetite. Ash was detected in riverbed sediment from quiet water settings such as inside of meander bends, the confluence of tributary streams and near islands between the spill site and 20 miles downstream in the Schoolfield Reservoir, Danville, VA. The strong magnetic signal is detected above background in riverbed samples and is strongly positively correlated with total ash; elevated low field magnetic susceptibility (χ _LF) is evident in samples with ≥ 12% ash content. Anhysteretic remanent magnetization and hysteresis parameters delineate native sediment, ash-bearing sediment, and diabase dikes. Between 20 and 70 miles downstream of the spill site, ash concentrations were either buried or too low due to dilution with native sediment to be detected with χ _LF in riverbed samples.     

翼龙类翼膜的折叠，褶皱以及物质特性

Preservation of wing membranes in pterosaurs is only reported from a few localities in the world, namely from the Karatau Hills, Kazakhstan (Upper Jurassic), from the Solnhofen Lithographic Limestone, Germany (Upper Jurassic), the Daohugou Beds of China (Upper Jurassic or Lower Cretaceous) and the Crato and Santana Formations, Chapada do Araraipe, NE Brazil (Lower Cretaceous).     

Gravitational spreading causes en-echelon diking along a rift zone of Madeira Archipelago: an experimental approach and implications for magma transport

Many volcanic rift zones show dikes that are oriented oblique rather than parallel to the morphological ridge axis. We have evidence that gravitational spreading of volcanoes may adjust the orientation of ascending dikes within the crust and segment them into en-echelon arrays. This is exemplified by the Desertas Islands which are the surface expression of a 60 km long submarine ridge in southeastern Madeira Archipelago. The azimuth of the main dike swarm (average = 145°) deviates significantly from that of the morphological ridge (163°) defining an en-echelon type arrangement. We propose that this deviation results from the gravitational stress field of the overlapping volcanic edifices, reinforced by volcano spreading on weak substratum. We tested our thesis experimentally by mounting analogue sand piles onto a sand and viscous PDMS substratum. Gravitational spreading of this setup produced en-echelon fractures that clearly mimic the dike orientations observed, with a deviation of 10°–32° between the model’s ridge axis and that of the main fracture swarm. Using simple numerical models of segmented dike intrusion we found systematic changes of displacement vectors with depth and also with distance to the rift zone resulting in a complex displacement field. We propose that at depth beneath the Desertas Islands, magmas ascended along the ridge to produce the overall present-day morphology. Above the oceanic basement, gravitational stress and volcano spreading adjusted the principal stress axes’ orientations causing counterclockwise dike rotation of up to 40°. This effect limits the possible extent of lateral dike propagation at shallow levels and may have strong control on rift evolution and flank stability. The results highlight the importance of gravitational stress as a major, if not dominant factor in the evolution of volcanic rift zones.Editorial responsibility: M Carroll     

Soil respiration under three different land use types in a tropical mountain region of China

Soil respiration (SR) Wis one of the largest contributors of terrestrial CO_2 to the atmosphere.Environmental as well as physicochemical parameters influence SR and thus, different land use practices impact the emissions of soil CO_2. In this study, we measured SR, bi-monthly, over a one-year period in a terrace tea plantation, a forest tea plantation and a secondary forest, in a subtropical mountain area in Xishuangbanna, China. Along with the measurement of SR rates, soil characteristics for each of the land use systems were investigated. Soil respiration rates in the different land use systems did not differ significantly during the dry season, ranging from2.7±0.2 μmol m~(-2) s~(-1) to 2.8±0.2 μmol m~(-2) s~(-1). During the wet season, however, SR rates were significantly larger in the terrace tea plantation(5.4±0.5 μmol m~(-2)s~(-1)) and secondary forest(4.9±0.4 μmol m~(-2)s~(-1)) than in the forest tea plantation(3.7±0.2 μmol m~(-2) s~(-1)).This resulted in significantly larger annual soil CO_2 emissions from the terrace tea and secondary forest,than from the forest tea plantation. It is likely that these differences in the SR rates are due to the 0.5times lower soil organic carbon concentrations in thetop mineral soil in the forest tea plantation, compared to the terrace tea plantation and secondary forest.Furthermore, we suggest that the lower sensitivity to temperature variation in the forest tea soil is a result of the lower soil organic carbon concentrations. The higher SR rates in the terrace tea plantation were partly due to weeding events, which caused CO_2 emission peaks that contributed almost 10% to the annual CO_2 flux. Our findings suggest that moving away from heavily managed tea plantations towards low-input forest tea can reduce the soil CO_2 emissions from these systems. However, our study is a casestudy and further investigations and upscaling are necessary to show if these findings hold true at a landscape level.     

Fluid–Rock Interactions in Geothermal Reservoirs,Germany: Thermal Autoclave Experiments Using Sandstones and Natural Hydrothermal Brines

As renewable energy, geothermal can contribute substantially to the energy transition. To generate electricity or to harvest heat, high-saline fluids are tapped by wells of a few kilometres and extracted from hydrothermal reservoirs. After the heat exchanger units have been passed by, these fluids are reinjected into the reservoir. Due to the pressure and temperature differences between the subsurface and the surface, as well as the cooling of the fluids in the power plant, unwanted chemical reactions can occur within the reservoir, in the borehole, and within the power plant itself. This can reduce the permeability of the reservoir as well as the output of the geothermal power plant. This study aims to simulate real subsurface reactions using batch and leaching experiments with sandstone or sandstone powder as solid phase, and deionised water or natural brine as liquid phase. It is demonstrated that fluid composition changes after only a few days. In particular, calcite, aragonite, clay minerals, and zinc phases precipitate from the natural brine. In contrast, in particular minerals containing potassium, arsenic, barium, and silica are dissolved. Due to the experimental set-up, these mineral reactions mainly took place on the surface of the samples, which is why no substantial changes in petrophysical properties could be observed. However, it is assumed that the observed reactions on the reservoir scale have a relevant influence on parameters such as permeability.