Impacts of elevated CO2 on particulate and dissolved organic matter production: microcosm experiments using iron-deficient plankton communities in open subarctic waters

Response of phytoplankton to increasing CO₂ in seawater in terms of physiology and ecology is key to predicting changes in marine ecosystems. However, responses of natural plankton communities especially in the open ocean to higher CO₂ levels have not been fully examined. We conducted CO₂ manipulation experiments in the Bering Sea and the central subarctic Pacific, known as high nutrient and low chlorophyll regions, in summer 2007 to investigate the response of organic matter production in iron-deficient plankton communities to CO₂ increases. During the 14-day incubations of surface waters with natural plankton assemblages in microcosms under multiple pCO₂ levels, the dynamics of particulate organic carbon (POC) and nitrogen (PN), and dissolved organic carbon (DOC) and phosphorus (DOP) were examined with the plankton community compositions. In the Bering site, net production of POC, PN, and DOP relative to net chlorophyll-a production decreased with increasing pCO₂. While net produced POC:PN did not show any CO₂-related variations, net produced DOC:DOP increased with increasing pCO₂. On the other hand, no apparent trends for these parameters were observed in the Pacific site. The contrasting results observed were probably due to the different plankton community compositions between the two sites, with plankton biomass dominated by large-sized diatoms in the Bering Sea versus ultra-eukaryotes in the Pacific Ocean. We conclude that the quantity and quality of the production of particulate and dissolved organic matter may be altered under future elevated CO₂ environments in some iron-deficient ecosystems, while the impacts may be negligible in some systems.     

Aseismic fault movement before the 1995 Kobe earthquake detected by a GPS survey: implication for preseismic stress localization?

By inversion analysis of the baseline changes and horizontal displacements observed with GPS (Global Positioning System) during 1990–1994, a high-angle reverse fault was detected in the Shikoku-Kinki region, southwest Japan. The active blind fault is characterized by reverse dip-slip (0.7±0.2  m yr⁻¹ within a layer 17–26  km deep) with a length of 208±5  km, a (down-dip) width of 9±2  km, a dip-angle of 51°±2° and a strike direction of 40°±2° (NE). Evidence from the geological investigation of subfaults close to the southwestern portion of the fault, two historical earthquakes ( M _L=7.0, 1789 and 6.4, 1955) near the centre of the fault, and an additional inversion analysis of the baseline changes recorded by the nationwide permanent GPS array from 18 January to 31 December 1995 partially demonstrates the existence of the fault, and suggests that it might be a reactivation of a pre-existing fault in this region. The fact that hardly any earthquakes ( M _L>2.0) occurred at depth on the inferred fault plane suggests that the fault activity was largely aseismic. Based on the parameters of the blind fault estimated in this study, we evaluated stress changes in this region. It is found that shear stress concentrated and increased by up to 2.1 bar yr⁻¹ at a depth of about 20  km around the epicentral area of the 1995 January 17  Kobe earthquake ( M _L=7.2, Japan), and that the earthquake hypocentre received a Coulomb failure stress of about 5.6 bar yr⁻¹ during 1990–1994. The results suggest that the 1995  Kobe earthquake could have been induced or triggered by aseismic fault movement.     

Crustal strain observation for nine years with a laser strainmeter in Kobe, Japan

We carried out precise crustal strain observation using a laser strainmeter system at the Rokko-Takao station in Kobe, Japan from 1989 to 1997. The long-term strain record is characterized by remarkable annual changes of the order of 2–3×10⁻⁶ and linear strain accumulation of −4.4×10⁻⁷/year (in contraction). The annual strain changes are inversely proportional to temperature changes that precede the strain changes by about 1 month. The apparent annual strain changes were mainly caused by refractive-index changes in the light path due to the ambient temperature changes. After eliminating the annual temperature effect, linear strain accumulation is corrected to be −6.3−6.7×10⁻⁷/year. Residual strains show the oscillating behavior, in which the oscillating cycle seems to become shorter and shorter as time goes by. During the period, a destructive earthquake of M=7.2 occurred near the Kobe City on 17 January 1995. We investigated the oscillating behavior in secular variations of ground-strains by introducing the deterministic approach of earthquake prediction to search for the “critical point” of the occurrence of an earthquake in the extended power law equation. However, we could not obtain a unique solution to determine eight unknown parameters including the “critical point”. This may be mainly due to lack of data for 4 months from August to November in 1994 by the failure of the laser source before the occurrence of earthquake on 17 January 1995. After removing environmental effects and tidal components, we carefully re-examined strain changes in 7 days and 1 day before the occurrence of the earthquake, but we could not detect anomalous strain changes exceeding 1×10⁻⁸ before the earthquake.     

Mesozooplankton responses to iron-fertilization in the western subarctic Pacific (SEEDS2001)

A mesoscale iron-fertilization experiment was carried out in the western subarctic Pacific during summer 2001. The iron-patch was traced for 14 days after the fertilization, and the abundance and behavior of mesozooplankton were compared with those outside of the patch. The phytoplankton biomass in the patch rapidly increased to over 15 times the initial level by the later half of the observation period, and was composed of large-sized (>10 mm), centric diatoms. Dominant zooplankton species in the upper 200-m depth were large copepods: Neocalanus plumchrus, Neocalanus cristatus, Eucalanus bungii and Metridia pacifica. Mesozoplankton biomass as well as species composition did not change significantly in the patch over the observation period. Furthermore, no changes of vertical distribution or diel vertical migration were observed for any species or stages of mesozooplankton throughout the observation period. However, the abundance of the first copepodite stages of N. plumchrus and E. bungii increased several fold in the patch after the diatom bloom formation compared to the densities outside the patch. The increases of both species are considered to be due to lowered mortality during the egg and nauplius stages. Spawning of N. plumchrus takes place at depth using lipid storage, while spawning of E. bungii takes place in the surface layer supported by grazing. These facts suggest that the relative importance of nauplii in the diets of the large copepods was decreased in the patch by the diatom bloom. Gut-pigment contents of dominant copepods in the patch increased 4–18 times, and the maximum values were observed during the bloom peak. However, the grazing impact on phytoplankton was low throughout the experiment, especially during the bloom period (<6% of the primary production).     

Dissolved iron and zinc in Sagami Bay and the Izu-Ogasawara Trench

Using a clean seawater sampling system for trace metals onboard the R. V. Shinsei-Maru, newly launched in 2013, we investigated the vertical distributions of dissolved iron and zinc in Sagami Bay and the Izu-Ogasawara Trench. We applied appropriate clean sampling and filtering processes for trace metals, so that uncontaminated seawater samples were successfully collected. The distribution of zinc in the trench area was similar to that of silicate and the same as that previously reported in the subtropical North Pacific. There were spatial variations in the iron (Fe) distribution in the trench areas. We used previously reported information about biogeochemical cycling in the trench area, and found that Fe has a residence time of 29 years in the water column. The short residence time of Fe (29 years) corresponds to the vertical variations of dissolved Fe in the water column.     

Iron nutritional status of the phytoplankton assemblage in the Okhotsk Sea during summer

To clarify the iron (Fe) nutritional status of the phytoplankton assemblage in the Okhotsk Sea, we conducted incubation experiments in summer 2006. Replicate surface seawater samples with the natural plankton community were incubated with three treatments: Fe enrichment; addition of the strong Fe chelator siderophore desferriferrioxime B (DFB) which strips Fe from the biologically accessible pool; and as a control, no addition. To prevent macronutrient limitation, we added surplus nutrients to all treatments. At all 4 stations in Sakhalin Bay near the mouth of the Amur River and around the east of Sakhalin Island, net specific growth rate showed no significant difference between the control and +Fe treatment, and was repressed in +DFB treatment both in large- and small-sized phytoplankton. These findings indicate that these waters contain sufficient bioavailable Fe and that the Amur River plume which is transported by the east Sakhalin current is a major source of the Fe. In the Bussol’ Strait, net specific growth rate in the control was significantly higher than +DFB treatment, suggesting a supply of bioavailable Fe through intense vertical mixing at this site. Iron enrichment treatment stimulated the net specific growth rate of large-sized phytoplankton, indicating that Fe still limits the growth for the large-sized phytoplankton assemblage, but not for small-sized phytoplankton, in this area. An index of Fe availability was defined to quantify the degree of ambient Fe availability in each station, and it revealed the spatial variability of ambient Fe availabilities among the sites.     

A 100 m laser strainmeter system installed in a 1 km deep tunnel at Kamioka, Gifu, Japan

We have installed a laser strainmeter system in a deep tunnel about 1,000 m below the ground surface at Kamioka, Gifu, Japan. The system consists of three types of independent interferometers: (1) an EW linear strainmeter of the Michelson type with unequal arms, (2) an NS-EW differential strainmeter of the Michelson type with equal arms and (3) a NS absolute strainmeter of the Fabry–Perot type. These are configured in L-shaped vacuum pipes, each of which has a length of 100 m. (1) and (2) are highly sensitive (order of 10⁻¹³ strain) and have wide dynamical range (10⁻¹³–10⁻⁶). Observations with strainmeters (1) and (2) started on June 11, 2003. (3) is a new device for absolute-length measurements of the order of 10⁻⁹ of a long-baseline (100 m) Fabry–Perot cavity by the use of phase-modulated light. This third strainmeter will be ready for operation before the end of 2004. The laser source of strainmeters (1) and (2) is a frequency-doubled YAG laser with a wavelength of 532 nm. The laser frequency is locked onto an iodine absorption line and a stability of 2 × 10⁻¹³ is attained. The light paths of the laser strainmeter system are enclosed in SUS304 stainless steel pipes. The inside pressure is kept to be 10⁻⁴ Pa. Consequently, quantitative measurement of crustal strains of the order of 10⁻¹³ can be attained by employing the laser strainmeter system of (1) and (2) at Kamioka. This resolving power corresponds to that of a superconducting gravimeter. Using the laser strainmeter system, we expect to determine parameters related to fluid core resonance, core modes and core undertone as well as other geodynamic signals such as slow strain changes caused by silent earthquakes or slow earthquakes.